glycosylation mutant
Defects in the N-Linked Oligosaccharide Biosynthetic Pathway in a Trypanosoma brucei Glycosylation Mutant
... procyclin glycosylation site is significantly less than that of procyclin in WT cells, which is virtually 100% ...4-1 mutant cells, on the other hand, despite expressing altered N-glycans with the same ...
9
A Glycosylation Mutant of Trypanosoma brucei Links Social Motility Defects In Vitro to Impaired Colonization of Tsetse Flies In Vivo
... We next investigated whether a lack of Rft1 affected the ability of the knockout to establish midgut infections. To date, a knock- out of GPI8, the transamidase that transfers the preformed GPI anchor to protein ...
5
Zika Virus Encoding Nonglycosylated Envelope Protein Is Attenuated and Defective in Neuroinvasion
... The glycosylation mutant ZIKVs are defective in ...the mutant ZIKVs are either defective in neurotropism or compromised for neuroinvasion when inoculated by the parenteral route ...the mutant ...
16
Essential Role of Dengue Virus Envelope Protein N Glycosylation at Asparagine-67 during Viral Propagation
... a glycosylation site at position 67 into WNV E protein has been shown to enhance DC-SIGN utilization ...DV glycosylation mutants lacking Asn-153 also showed a reduced infectivity in Raji-DCs ...this ...
13
Identification of the Optimal DC-SIGN Binding Site on Human Immunodeficiency Virus Type 1 gp120
... N-linked glycosylation (N-glycan) sites in gp120 that contribute to optimal DC-SIGN ...multiple mutant forms of the 2G12 epitope revealed one triple glycosylation mutant form, termed 134mut ...
12
Coordinate Deletion of N-Glycans from the Heptad Repeats of the Fusion F Protein of Newcastle Disease Virus Yields a Hyperfusogenic Virus with Increased Replication, Virulence, and Immunogenicity
... N-linked glycosylation of the NDV F protein in viral biological activities and viral pathogenesis using a previously described reverse genetics system for the meso- genic strain Beaudette C (BC) ...N-linked ...
11
Intracranial Hemorrhage as the Initial Manifestation of a Congenital Disorder of Glycosylation
... of glycosylation are a genetically and clinically heterogeneous group of multisystem disorders characterized by the abnormal glycosylation of a number of ...abnormal glycosylation of various ...
10
The transgenic expression of LARGE exacerbates the muscle phenotype of dystroglycanopathy mice
... Dystroglycan (DG) forms the central component of the dystrophin associated protein complex and has been attributed with a primary role in the deposition, organisation and turnover of basement membranes (1-7). It is ...
59
Bridging the Gap between Glycosylation and Vesicle Traffic
... of glycosylation, the characteristic reduction in sialylation and galactosylation often seen in COG-CDG patients would emerge as a result while minimal change to the oligomannose processing would occur in contrast ...
13
N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing
... the glycosylation patterns of the major influenza virus antigen, hemagglutinin ...the glycosylation patterns of seven reference antigens containing HA used in influenza vaccine potency ...peptide ...
22
Unconventional N Linked Glycosylation Promotes Trimeric Autotransporter Function in Kingella kingae and Aggregatibacter aphrophilus
... HMW1 is an adhesive protein that mediates interaction be- tween bacterial cells and human epithelial cells and is encoded by a two-partner secretion system gene cluster that also encodes HMW1B (an outer membrane ...
9
Glycan Microheterogeneity at the PGT135 Antibody Recognition Site on HIV-1 gp120 Reveals a Molecular Mechanism for Neutralization Resistance
... important parameter for the efficacy of glycan-reactive bnAbs. This is an important consideration for vaccine design, since any elicited bnAb response will likely require a degree of plasticity in glycan recognition in ...
8
Single amino acid substitutions in the hemagglutinin can alter the host range and receptor binding properties of H1 strains of influenza A virus.
... Properties of WSN HA variantsa Glycosylation sequon of Virus Origin Biological properties residu stranresidue: Parent virus isolated from CEFgrown WSN stock Cf -1 Mutant virus selected f[r] ...
7
Mutational Analysis of the N-Linked Glycans onAutographa californica Nucleopolyhedrovirus gp64
... M13 mutant was checked by sequencing single-stranded DNA via the chain termination method (40); then the mutated gp64 fragments were excised and used to replace the corresponding wild-type fragment in ...single ...
11
Hepatitis B Virus Large Envelope Protein Interacts with γ2-Adaptin, a Clathrin Adaptor-Related Protein
... L mutant pro- tein that had been shown to be unable to dispose its pre-S domain to the ...Ile-9::L mutant hence appeared in non-, single-, double-, and triple-glycosylated forms, as appar- ent from ...
9
The N-glycan of the SCR 2 region is essential for membrane cofactor protein (CD46) to function as a measles virus receptor.
... with glycosylation inhibitors have indicated that for virus binding it is not important whether the N-glycans are present as complex or mannose-rich oligosaccharides ...
5
Covalent Modifications of the Ebola Virus Glycoprotein
... (MORPH). Mutant DNAs were used to transform Esch- erichia coli mismatch repair mutants (BMH 71-18 or MORPH mutS cells), and miniprep DNA was isolated (5 Prime 3 3 Prime) and used in the second-round transformation ...
10
25 Hydroxycholesterol Inhibition of Lassa Virus Infection through Aberrant GP1 Glycosylation
... ABSTRACT Lassa virus (LASV) infection is a major public health concern due to high fatality rates and limited effective treat- ment. The interferon-stimulated gene cholesterol 25-hydroxylase (CH25H) encodes an enzyme ...
9
Low amounts of bisecting glycans characterize cerebrospinal fluid-borne IgG
... 1985), glycosylation patterns of IgG in serum have been extensively researched in physiological and pathological ...N- glycosylation patterns change during acute inflammation and aging but also during ...
18
Hallmarks of glycosylation in cancer
... [151]. Glycosylation of IgG is known to play a role in tumour immune surveillance and is being investigated as a diagnostic marker in several cancer types ...altered glycosylation using anticancer vaccines ...
12