gradient gel electrophoresis profile
Structural Shifts of Mucosa Associated Lactobacilli and Clostridium leptum Subgroup in Patients with Ulcerative Colitis
... Denaturing gradient gel electrophoresis analysis was employed to profile the composition of the dominant bacteria (16S rRNA gene V3 region) and three important groups: lactobacilli, the ...
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Polymerase Chain Reaction Denaturing Gradient Gel Electrophoresis (PCR DGGE) Profile of Bacterial Community from Agricultural Soils in Bauchi, North East Nigeria
... PCR amplification of 16S r RNA genes was carried out using the forward pri- mer/63F and reverse primer/1387R. The amplification reaction was performed with ESCO-MAXI swift thermocycler PCR machine. PCR reaction on DNA ...
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Effect of legume cereal mixtures on the diversity of bacterial communities in the rhizosphere
... Denaturing gradient gel electrophoresis (DGGE) of 16S rDNA fragments was used to profile the structure of the bacterial community in the ...
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Denaturing Gradient Gel Electrophoresis of PCR Amplified gki Genes: a New Technique for Tracking Streptococci
... Therefore, electrophoresis of amplified fragments provides band patterns specific for different bacterial ...temperature profile: 95°C for 5 min; 5 cycles at 25°C for 1 min and 72°C for 1 min; and 40 cycles ...
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Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles
... be interesting to follow these subjects, monitor their profile, and record which clades lead to the inception of periodontal disease. However, this analysis technique is limited by the fact that it does not take ...
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Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR
... DGGE gel separated V3 16S rRNA genes from different bacteria ...DGGE profile, the COPD group shared a band at the same position, whereas there was nearly no band at the corresponding place in the healthy ...
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Rapid Discrimination of Listeria monocytogenes Strains by Microtemperature Gradient Gel Electrophoresis
... Here, -TGGE analysis was applied to the discrimination and general identification of L. monocytogenes strains. The PCR-amplified region was selected by comparison of the ge- nomes of L. monocytogenes serovar 4b strains ...
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Search for Localized Dysbiosis in Crohn's Disease Ulcerations by Temporal Temperature Gradient Gel Electrophoresis of 16S rRNA
... a profile of the dominant bacterial species present in a sample but does not identify the species individ- ually (16, 24, ...species profile of a sample in relation to different ...
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Metagenomic rDNA Profiles of the Oral Microbiome in Healthy Subjects Are Distinctive Compared to That in Smokers and Disease Subjects Despite High Variations between Subjects
... tured gradient gel electrophoresis (PCR-DGGE) of the 16S rDNA gene in me- tagenomic samples, this study evaluated the stability of the oral microbiome and contrasted the PCR-DGGE profiles of subjects ...
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Characterization of endemic Shigella boydii strains isolated in Iran by serotyping, antimicrobial resistance, plasmid profile, ribotyping and pulsed field gel electrophoresis
... mid profile analysis was able to differentiate seven pat- terns among 10 isolates: P6 only was shared by four strains, while the remaining plasmid profiles were ...plasmid profile anal- ysis showed an ...
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An investigation into the potential impacts of ocean acidification and ocean fertilisation on the genetic diversity of marine bacterial assemblages
... Whole community profiles can be obtained using only the terminal restriction fragments which are fluorescently labelled. Terminal RFLP (TRFLP), as it is known, gives an accurate community profile based on the ...
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Diversity of the gut microbiota and eczema in early life
... denaturing gradient was formed by using two 6% polyacrylamide stock solu- tions containing a 20–55% urea/formamide gradient that increases in the direction of ...used. Electrophoresis was performed ...
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Two dimensional Polyacrylamide Gel Electrophoresis pdf
... polyacrylamide gel matrix restric- tion; (iv) ampholyte } protein interactions may alter the observed pI of the ...pH gradient elec- ...pH gradient is created by copolymerization of acrylamide / N,N ...
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Evidence of melanoma in wild marine fish populations
... verified by agarose gel electrophoresis [1.6% (w/v) agarose] with ethidium bromide staining and visualized using a UV transillumi- nator. DGGE was performed using the D-Code universal mutation detection ...
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Persistence of orally administered Megasphaera elsdenii and Ruminococcus bromii in the rumen of beef cattle fed a high grain (barley) diet
... A subsequent study (Klieve et al. 2007 ) using denaturing gradient gel electrophoresis (DGGE) to identify the dominant bacteria in ruminal contents of these cattle found that the commonl[r] ...
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Evaluation of Denaturing Gradient Gel Electrophoresis (DGGE) for the Determination of Marine Phytoplankton Genetic Diversity
... The gel contained 8% (w/v) polyacrylamide ...linear gradient of 0% - 70% denaturant was established from left to ...The gel was polymerised with ...the gel. The gel was electrophoresed ...
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Temperature Gradient Gel Electrophoresis as a Valuable Accessory Tool for Assessment of Dysbiosis in Crohn’s Disease
... family. However, the technique has also its limitations, the most evident of which seem to be occasional lack of data reproducibility due to gel manufacture and silver staining variations. In any case, taking in ...
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Capillary Gel Electrophoresis pdf
... Electrophoretic separation is based on differences in the charge density (the mass-to-charge ratio) of the analytes. Small, highly charged molecules move faster than large, low charged ones, while uncharged mol- ecules ...
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Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease
... in-gel electrophoresis; 2D PAGE: Two dimensional polyacrylamide gel electrophoresis; ANOVA: Analysis of variance analysis; CHAPS: 3-((3-Cholamidopropyl) dimethylammonio)-1- propanesulfonate; ...
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The impact of growth hormone on proteomic profiles: a review of mouse and adult human studies
... difference gel electrophoresis; 2DE: 2‑dimensional gel electrophoresis; 2DE + MS/MS: 2‑dimensional gel electro‑ phoresis plus tandem mass spectrometry; A2M: alpha‑2‑macroglobulin; AAT: ...
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