Hepatitis B virus polymerase
Evidence for activation of the hepatitis B virus polymerase by binding of its RNA template.
... The hepatitis B viruses replicate by reverse transcription of an RNA pregenome by using a virally encoded ...the polymerase to an RNA stem-loop ( « ) of the pregenome; « is both the RNA encapsidation ...
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Human Hepatitis B Virus Polymerase Interacts with the Molecular Chaperonin Hsp60
... that hepatitis B virus polymerase (HBV Pol) interacts with host factors such as the Hsp90 complex, which is a critical step in viral genome ...
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The Duck Hepatitis B Virus Polymerase Is Activated by Its RNA Packaging Signal, ɛ
... the hepatitis B viruses is both the primary element of the RNA packaging signal and the origin of reverse ...the polymerase is proposed to induce a physical alteration to the polymerase that ...
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Effects of insertional and point mutations on the functions of the duck hepatitis B virus polymerase.
... To assist in the assignment of these functions to domains of the duck hepatitis B virus polymerase protein, we have constructed a series of substitution mutations and a large insertion m[r] ...
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Protein-Primed Terminal Transferase Activity of Hepatitis B Virus Polymerase
... the polymerase active site per se in- duces this conformational change, independent of any template, that leads to a gain of PFA sensitivity (likely corresponding to a “generic” elongation mode of DNA ...
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Properties of Monoclonal Antibodies Directed against Hepatitis B Virus Polymerase Protein
... transfected by HBV constructs (of more than one genome length) that allow for viral DNA synthesis were analyzed with MAb 2C8, 8D5, or 9F9, no signals corresponding to HBV Pol could be detected, while viral core and ...
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Mapping of Functional Subdomains in the Terminal Protein Domain of Hepatitis B Virus Polymerase
... ABSTRACT Hepatitis B virus (HBV) encodes a multifunction reverse transcriptase or polymerase (P), which is composed of several domains. The terminal protein (TP) do- main is unique to HBV and ...
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Effects of Genomic Length on Translocation of Hepatitis B Virus Polymerase-Linked Oligomer
... DNA polymerase (P), and X protein ...The polymerase-linked oligomer priming site is located at the bulge region of ...endogenous polymerase reaction with cold deoxynucleoside triphosphates (12) were ...
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Duck hepatitis B virus polymerase acts as a suppressor of core protein translation.
... Constructs. Plasmid pD2Eco11 was constructed from an infectious DHBV-16 virion genome isolated from the serum of a DHBV-infected duckling. It contains a complete genome of DHBV DNA fused to a pUC19 vector at the EcoRI ...
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Inhibition of Hepatitis B Virus Polymerase by Entecavir
... Entecavir (ETV; Baraclude) is a novel deoxyguanosine analog with activity against hepatitis B virus (HBV). ETV differs from the other nucleoside/tide reverse transcriptase inhibitors approved for HBV ...
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Large-Scale Production and Structural and Biophysical Characterizations of the Human Hepatitis B Virus Polymerase
... FIG 2 Reappraisal of the boundaries of hPOL structural domains. (A) Annotation of secondary structure predictions based on HCA (gray symbols above the schematic presentation of classical hPOL domain boundaries) and using ...
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The Impact of the Hepatitis B Virus Polymerase rtA181T Mutation on Replication and Drug Resistance Is Potentially Affected by Overlapping Changes in Surface Gene
... drug-resistant hepatitis B virus (HBV) is a major problem for antiviral treatment in chronic hepatitis B infec- ...HBV polymerase gene from serum samples during viral ...
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Hepatitis B Virus Polymerase Localizes to the Mitochondria, and Its Terminal Protein Domain Contains the Mitochondrial Targeting Signal
... of hepatitis B virus (HBV) replication, we visualized core (Cp), polymerase (Pol), and pre- genomic RNA (pgRNA) in infected ...duck hepatitis B virus (DHBV) also localized ...
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Characterization of Hepatitis B Viral Drug Resistance in Indian Subcontinent Patients with Chronic Liver Disease.
... DNA virus of the family ...DNA polymerase, reverse transcriptase (rt) and other accessory ...and hepatitis B surface antigen (HBsAg) and is completely located within the ORF ...for ...
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Hepatitis B virus : a longitudinal study : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science at Massey University, Palmerston North, New Zealand
... sequences. First the double stranded DNA template is separated using a high temperature of 94-96°C. This is followed by a rapid drop to 45-60°C for 30-60 seconds to allow the oliogonudeotide primers to anneal to the ...
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Naturally occurring missense mutation in the polymerase gene terminating hepatitis B virus replication.
... Transfection of HuH-7 cells with these clones, followed by Southern blot analyses of purified core particles, demonstrated that the A-to-C mutation of clone adw R9 in position 2798 rende[r] ...
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Transcomplementation of nucleotide priming and reverse transcription between independently expressed TP and RT domains of the hepatitis B virus reverse transcriptase.
... the polymerase Met at nucleotide (nt) 2309 ...a virus encoding the first 334 amino acids of pol with nine additional non-pol amino acids and still containing all downstream HBV RNA sequences, including DR1 ...
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DNA Polymerase in the Core of the Human Hepatitis B Virus Candidate
... Thus, the NP-40 treatment, the DNA polymerase activity [3H]DNA product could be precipitated directly was found at the same position in a sucrose from the NP-40-treated enzyme reaction m[r] ...
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Hepatitis D virus and hepatitis B virus infection in Amerindian communities of the Amazonas state, Colombia
... HDV: Hepatitis D virus; HBV: Hepatitis B virus; SIVIGILA: Surveillance system of public health in Colombia; HBsAg: Hepatitis B surface Antigen; anti-HBc: Antibodies anti ...
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Direct method for detecting small quantities of hepatitis B virus DNA in serum and plasma using the polymerase chain reaction
... microliters serum may be directly detected by PCR without nucleic acid extraction. The sensitivity is dependent on the surface area of the adsorptive surface and is increased by having antibodies on the adsorptive ...
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