Hepatocyte nuclear factor-1, -3, -4
A NOVEL MUTATION IN THE HEPATOCYTE NUCLEAR FACTOR-1 Α GENE AMONG MODY EGYPTIAN PATIENTS
... mutations 4/22 (I27L, E105G, E90V and S487N), and heterozygous mutations 9/22 (P325D, P334S, V342G, T346P, P347S, S359R, S361N, L362Q and ...domain 1/22 ...exons 1 in three patients (2, 3 and ...
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Regulation of Hepatocyte Nuclear Factor 1 Activity by Wild-Type and Mutant Hepatitis B Virus X Proteins
... Hepatitis B virus (HBV) is a liver-tropic virus. It can cause acute and chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. HBV is a DNA virus with a 3.2-kb, partially double- stranded genome. This genome ...
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A review on hepatocyte nuclear factor-1beta and tumor
... peptidase 4 (DPP4), and osteopontin (SPP1) are most likely direct target genes, as they contain functional HNF1 binding sites in their promoter ...beta 1) is a member of noncollagenous con- stituent of ...
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Mutations in the coding regions of the hepatocyte nuclear factor 4 alpha in Iranian families with maturity onset diabetes of the young
... Hepatocyte nuclear factor 4α (HNF4α) is a nuclear receptor involved in glucose homeostasis and is required for normal β cell ...and 3 of 21 individuals in ...members, 3 subjects ...
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Regulation of transcription from the hepatitis B virus large surface antigen promoter by hepatocyte nuclear factor 3.
... ( 1 HNF3 b ) of the HNF3 b ex- pression vector ...transcription factor- binding sites cloned into pHBVTATALUC did not show tran- scriptional transactivation by exogenously expressed HNF3 b ...
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A missense mutation in hepatocyte nuclear factor 4 alpha, resulting in a reduced transactivation activity, in human late onset non insulin dependent diabetes mellitus
... Since the V393I substitution does not result in the gain or loss of a site for a restriction endonuclease, we developed a simplified protocol that would allow us to use a PCR-RFLP as- say to test for its presence (Fig. ...
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Role of hepatocyte nuclear factor 4 alpha in cell proliferation and gemcitabine resistance in pancreatic adenocarcinoma
... We first used IHC to examine the HNF4α levels in 30 paired-patient samples of PDAC and adjacent normal tissues (Fig. 1a, b). Then, we further validated the IHC results by examining HNF4α expression in tissue micro- ...
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Study of the expression levels of Hepatocyte nuclear factor 4 alpha and 3 beta in patients with different outcome of HBV infection
... The immunohistochemical results of integrated tissue slice slides were read independently by two pathologists who had no prior knowledge of the experimental procedures. The percentage of positive cells and the positive ...
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Hepatocyte Nuclear Factor 3β Inhibits Hepatitis B Virus Replication In Vivo
... The hepatitis B virus (HBV) is a 42-nm enveloped particle possessing an internal 28-nm nucleocapsid (5, 14). The 3.2-kb partially double-stranded circular viral genome and the HBV polymerase are present within the ...
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Epigenomic signatures in liver and blood of Wilson disease patients include hypermethylation of liver-specific enhancers
... promoters hypermethylated in specific liver cell types were not associated with differential methylation in WD liver. A second limitation was the lack of liver controls from individuals of a healthy weight. This was ...
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Mesonephric adenocarcinoma of the cervix: a case report with a three-year follow-up, lung metastases, and next-generation sequencing analysis
... MNAC has a broad differential diagnosis. On the benign spectrum, it should be differentiated from MH and MR (See Table 2). Ki-67 immunostaining may be helpful, since it has been reported to show positivity in only ...
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Induction of an EMT like transformation and MET in vitro
... Confocal immunofluorescent analysis: Cells were fixed with paraformaldehyde for 30 minutes, washed three times with PBS then permeabilized with 0.1% Triton X- 100 for 10 minutes at room temperature, washed three times ...
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Defective insulin secretion in hepatocyte nuclear factor 1alpha deficient mice
... ( 1 / 1 ) mice exhibited a core of b cells with uni- form, strong insulin immunostaining surrounded by a mantle of non- b cells ...( 1 / 2 ) mice were similar to those from ( 1 / 1 ) ...
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Cholestatic jaundice in infancy: struggling with many old and new phenotypes
... (hepatocyte nuclear factor-1-beta) gene muta- ...transcription factor, also plays a pivotal role in organogenesis during embryonic ...
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Nuclear Klf4 accumulation is associated with cetuximab drug resistance and predicts poor prognosis of nasopharyngeal carcinoma
... on the cell membrane (Fig. 2A-c), while the nEGFR pro- tein was expressed on the cell membrane as well as in the nucleus. This study mainly observed the EGFR protein that was expressed in the nucleus (Fig. 2A-d). The ...
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Apoptosis signal-regulating kinase 1 mediates the inhibitory effect of hepatocyte nuclear factor-4α on hepatocellular carcinoma
... characteristic hepatocyte markers in hepatoma cells ...these hepatocyte marker genes (Figure 7C), seemed to mimic the function of ...of hepatocyte marker genes by ectopic HNF4α was also blocked by ...
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Liraglutide downregulates hepatic LDL receptor and PCSK9 expression in HepG2 cells and db/db mice through a HNF-1a dependent mechanism
... Twenty-four male db/db mice (BKS.Cg-Dock7m +/+ Leprdb/J, Strain Number: J000642) aged 5 weeks and sixteen non-diabetic littermates (wild-type, WT) were purchased from the Model Animal Research Center of Nanjing ...
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Unilateral hypoplastic kidney - a novel highly penetrant feature of familial juvenile hyperuricaemic nephropathy
... Upon initial examination the patient was noted to ap- pear well. His blood pressure of 122/55 was within normal centile range for his height and age. An ultrasound re- vealed a small right kidney measuring 8.2 cm which ...
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Intestinal apolipoprotein AI gene transcription is regulated by multiple distinct DNA elements and is synergistically activated by the orphan nuclear receptor, hepatocyte nuclear factor 4
... We have used apolipoprotein genes to investigate the signal transduction mechanisms involved in the control of intestinal specific gene expression. The human apoAI, apoCIII, and apoAIV genes are tandemly organized within ...
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Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening
... culture, hepatocyte-like cells differentiated from iHepLPCs (iHepLPCs-Hep) at passage 30 showed a gradual increase in the expression of drug metabolism enzyme CYP3A4, albumin, α-antitrypsin (AAT), urea cycle key ...
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