Herbo Mineral Formulation

Rheumatoid arthritis is an auto immune disorder associated with chronic inflammatory, destructive, and deforming symmetrical Polyarthritis associated with symmetrical involvement of arthritic efficacy of herbo-mineral formulation pooneeru arthritic activity of Pooneeru diravagam (in doses of 0.2 ml/kg and complete adjuvant in to the sub plantar were used as standard drug and This study includes examination of the paws thickness measurement, paw volume, paw withdrawal latency and Histopathological analysis. immunization with adjuvant. The drug injection (0 day), 30 minutes before was evaluated by the measurement of joint diameters of left hind paw were

The selected animals were divided in to three groups and each group consisted of six animals. Group-I: Served as normal control and received distilled water as vehicle; Group-II: Served as disease control and exposed to 0.06 mL, 50 % acetic acid for 60s; Group-III: Served as test drug (Herbo-mineral formula- tion) treated group and received Herbo-mineral formulation (100 mg/kg/day, p.o.) + exposed to acetic acid.

GC is a herbo mineral formulation. It consists of Gandhagam (Sulphur), and the other ingredients are coconut oil, cucumber, egg white. Honey was used as an vehicle to promote their activity. Gandhagam has been reported to have astringent action [1] . Gandhagam can increase skin [2] health and it act as essential dietary mineral used in skin health and overall wellness of the body. Gandhagam deficiency in our body leads to progression of inflammatory skin diseases. Psoriasis has been treated with Oral Sulphur along with zinc2. In

Ancient siddha literatures notified the diseases as 4448. Among them, the diseases pertaining to gunmam are 8. Eri gunmam is one of the 8 types of gunmam mentioned in yugi vaithiya cinthamani 800.[2] Gunmam is a clinical entity which depresses both body and mind since it is called as gunmam. The signs and symptoms of Eri Gunmam in siddha literature may be correlated with peptic ulcer disease in modern disease of classification.So it is considered to evaluate a classical siddha herbo mineral formulation “Arputha mathirai” mentioned in kosayee anuboga vaithiya brahma ragasiyam, part-II, [1] for the treatment of Eri Gunmam (Peptic ulcer). Peptic ulceration is one of the common disease affecting millions of people. It is now considered to be one of the modern age epidemics affecting nearly 10% of world population [3]. There is a rich

All the extracts used in Herbo-mineral formulation shows their organoleptic characters. Moisture content of all the ingredients was less than 5%, so that it can prevent microbial growth and sticking problem in final processing of formulation. The pH of all ingredients was near about to neutral. The extracts used in Glucova Active Tablet were water soluble. Concentration of total ash and acid insoluble ash in each ingredient was less than the limit. The quality control parameters of the finished product were also under the limit. Also the data of HPTLC fingerprinting of all extracts indicates that all extracts were derived from genuine plants or parts of plant. Concentration of heavy metals in the extracts of Herbo- mineral formulation is also in limit. Thus in Conclusion, data suggested that tablets and all its extracts were consistent with various quality and purity parameters such as organoleptic characters, physico-chemical parameter, HPTLC fingerprinting, heavy metals and microbial analysis. So from above data, it can be said that Herbo-mineral formulation has all the necessary ingredients that are active against Diabetes.

Siddha system of medicine is a renowned holistic system of traditional medicine emphasizing curative and preventative measures. The medicine used in Siddha are of plant origin, metals, minerals, and animal products. Most of the Siddha medicine has improvise the longevity of life through their anti–oxidant properties. The antioxidant activity of medicinal formulations and their roles in the prevention and treatment of various human chronic and degenerative diseases have attracted more and more attention. The drug “Rathi nagara rasa mezhugu” (RNM) is a herbo mineral formulation mentioned in the Siddha literature “Anuboga vaithya navaneetham part V” indicated for Penile cancer, Cervix cancer, Cervical adenitis, Leprosy, Syphilis, Rheumatoid Arthritis. Siddha system emphasis mainly on healthy long life by preventing ageing and degenerative disease with medicines having antioxidant activity. Even though this drug has been used based on traditional knowledge no scientific work has been done to evaluate the antioxidant properties of RNM based on various in vitro assays. The study results confirmed that the drug RNM has promising therapeutic antioxidant activity when compared with the standard drug. This research work can help for medical practitioners to use this herbo-mineral compound for the treatment of cancer.

Standardization of Siddda drugs is the need of the hour. ‘Mayilliragathy chooranam’ is a herbo -mineral siddha preparation, being used as Hiccups and vomiting in the text Theyraiyar padal thiratu-1001. Aim and Objective: To do physicochemical and phytochemical analysis for the drug ‘Mayilliragathy chooranam’. Materials and Methods: The drug is prepared as per the method mentioned in the classic siddha literature. The powder of this formulation was subjected to physicochemical study such as total ash value, acid insoluble ash, water soluble ash, water soluble extractive, Alcohol soluble extractive, moisture content. The behavior of the prepared powder with different reagent and fluorescence analysis was also carried out Preliminary phytochemical screening of the extracts showed the presence of alkaloid, carbohydrate, glycoside, diterpenes, flavonoids, gum& mucilage The physicochemical study reveals that drug contains the following composition: total ash 7.36%, acid insoluble ash 1.00%, water soluble ash 2.72% water soluble extractive 16.16%, Alcohol soluble extractive 31.59%, moisture content 8.73%,.The achieved results of physico-chemical, and phyto-chemical profiling will be useful as tools for authentication and standardization profile of the herbo-mineral formulation.

Aim: To evaluate acute oral toxicity of Anacid capsule (Herbo-Mineral formulation) on swiss albino mice and to evaluate ulcer protective and antioxidant effect of Anacid capsule against peptic ulcer in pylorus ligation induced ulcer model. Method: The present study was conducted according to OECD guideline AOT-425 to know single dose toxicity of Anacid capsule (polyherbal formulation) on swiss albino mice. The IAEC no. for the study is SKPCPER/IAEC/2016-02/01. The study was conducted using 5 swiss albino mice. The male and female animals were selected for study of 8 - 12 weeks old with weight range of within ± 20 % of mean body weight at the time of randomization. A limit dose of 2000 mg/kg of extract was used involving five mice. Each mouse was treated with a single oral dose of 2000 mg/kg of extract in sequence at 48 h intervals. Animals were observed individually at least once during the first 30 min after dosing, periodically during the first 24 h and daily thereafter, for a total of 14 days for any clinical signs of toxicity or mortality. Body weight of all animals was recorded once in a week. The ulcer protective effect of test drug was performed in Pylorus ligation induced ulcer model in Albino Wister Rats. The anti oxidant activities of Anacid capsule was carried out by its effect on various oxidative stress markers i.e. Superoxide dismutase (SOD),Catalase and Lipid peroxidation (LPO-MDA).Results: There were no physical and behavioral changes observed in swiss albino mice during 14 days. Body weight of all animals did not reveal any significant change as compared to vehicle control group. Mortality was not observed in any animal of a group. The statistically significant increase in pH and decrease in gastric volume, total acidity, free acidity and ulcer index was found in Anacid capsule treated group in compression to various control and standard drug treated group which proves potential ulcer protective and antacid effect of this combination. The results of test drug on oxidative stress markers favor its anti oxidant properties Conclusion: This study reveals that Anacid capsule (Herbo-Mineral formulation) does not have any toxic effect at dose of 2000 mg/kg. So No-Observed-Adverse-Effect-Level (NOAEL) of Anacid capsule is 2000 mg/kg. The obtained results suggest that, tested polyherbal formulation (Anacid Capsule) has antiulcer and anti oxidant effect without any major side effects or mortality.

Ayurvedic herbal medicines and their finished herbal products have been used since ancient times to treat a wide range of diseases. WHO survey shows 70-80% of world population rely on non-conventional medicines mainly herbal products for their primary health care. The microbial contaminations of these medicines may reduce or even inactivate the therapeutic activity of the products and has the potential to adversely affect patients. Tribhuvan Kirti Ras is one of the commonly used herbo-mineral formulation for acute or chronic fever related with respiratory tract infections, Present study was performed to assess the pathogenic proliferation in the locally available commercial Tribhuvan Kirti Ras. The pathogenic load was compared with the microbiological standard given by the WHO. The TVAC was found to be 9.3×10 4 , 4.5×10 4 and 5.8×10 4 CFU/g in three samples respectively, All three samples were found to be contaminated with the fungi species of Gliodium, Papulospora, Geomyces and Rhizopus as well as Gram positive and Gram negative bacteria. The presence of bacteria and fungi in these samples suggested that there is need to improve the quality of manufacturing and labelled the condition of product.

Objective: To perform qualitative and quantitative estimation of „Curcumin‟ in a Siddha herbo-mineral formulation – „SanthaSanthrodayamMathirai (SSM)‟ using High-Performance Thin Layer Chromatography (HPTLC). Method: Methanolic extracts of three samples of SSM (A, B, C) prepared using the traditional procedures described in Siddha literature. High Performance Thin Layer Chromatography method was performed for quantification of curcumin in all the three samples by employing chloroform: methanol 9.5:0.5 (v/v) as a mobile phase. Results: Curcumin was identified at UV- 366 nm using the Retention factor (R f ) values in the three samples

Swasananda Gulika is mentioned in Arogyaraksha Kalpadruma, as a remedy for Kasa, Swasaand Hikka. In present study Swasananda Gulika was prepared according to the Standard Operative Pro- cedures(SOPs) mentioned in classics. It is important that crude drugs of both herbal and mineral ori- gin should be subjected to Sodhana (purification) before using internally or in the preparation of any other compound form of drugs. The ultimate objective of sodhana process is to increase the bio- availability of the drug and further potentiating the biological efficacy. There are many methods for the sodhana process of each ingredient of Swasananda Gulika. Each process imparts specific proper- ties to the drugs. So it is a difficult task to select a proper sodhana method. Of the various sodhana methods, the method which is suitable for the preparation of Swasanandagulika was chosen here. To standardize a process, proper analysis should be done in each step. Analytical study of each sodhana procedure during the preparation of Swasanandagulikawas conducted in present study and observa- tions were noted carefully. In current study, best method for Hingulasodhana was done, in which hingula becomes physically more pure and after sodhana, the weight of purified hingula was found to be increased by 1.4 gm (3%). The weight gain may be due to the presence of organic matter in the bhavanadravya which was added during sodhana. It may be starch or other particles. During purifi- cation, sodhana drugs will attribute some properties to the drugs to be purified. In this study it has been observed that nimajjana in gomutra for 3 times, mentioned in the classics was found to be more accurate method for Vatsanabhasodhana. For the preparation of the medicine for Swasa, the drug of choice of Vatsanabhasodhana was gomutra compared to godugdha, since gomutra has Vatakapha- hara property. In this study it has been also found that, there was a weight gain of Swasanandagulika (18%), compared to the total weight of ingredients which may be from the Triphalakashaya added

Gulmakuthara Rasa-2 contains only the Bhasma’s of 4 metal and 1 mineral. Tamra Bhasma mainly does the Lek- hana Karma. A study has been carried out and concluded that Tamra Bhasma prepared out of Mulika media is more effective in decreasing the Serum VLDL cholesterol level and increasing the serum HDL cholesterol level. 21 This

The present study reveals that the heavy/ toxic met- als As, Pb, Cd and Hg were found to be absent, which ensured the safety of this formulation. XRD studies confirmed the alpha iron oxide phase and gamma iron oxide phases existed in the formulation. SEM analysis shows the aggregated particle mor- phology for the formulation. The above investigation on Ayakantha chenduram using modern techniques establishes the fingerprint for standardization of the herbal formulation. In this study Ayakatha chen- duram, a mineral based Siddha classical medicine is characterized scientifically for elemental composi- tion, structural and textural properties, morphology and crystalline structure by different characterization techniques. The EDAX analysis shows the presence of Carbon, Oxygen, Sodium, Silicon, Sulphur, Chlo- ride and Iron. FT-IR spectroscopy shows that the major stretching vibrations of different functional groups in organic compounds in the spectra have very low intensity. During the heating process in- volved in the preparation of the formulation, the or- ganic groups might have changed into gaseous oxi- dized compounds and might have escaped. The XRD

in ferrous form, vitamins C and E which fight against free radicals.Free radicals damage DNA, altering biochemical compounds, corroding cell membranes. In addition to that, for most iron-rich ingredients, it is essential to combine with vitamin C to promote assimilation in the body.The great thing about DDC is that this formulation contains both iron and vitamin C. In the view of many health benefits and iron rich contents leads to this study of haematinic activity of the trial drug DDCin phenyl hydrazine induced anaemic rats which may serve as a beacon light in treating anaemia.

Taleshwara rasa 1 is one such rasoushadhi which is mentioned in Rasa Manjari. It may be named after the main ingredient in the formulation i.eHaratala which is the best kushtahara dravya. It contains Shuddha Parada, Shuddha Gandhaka, Shuddha

Shilajit is a blackish brown exudation found in the surroundings of Himalayas. It is a mineral pitch that oozes from the rocks of the Himalayas, in the summer months. It is said to carry the healing power of these great mountains [1]. It is also found in most of the sedimentary rocks especially in Afghanistan, Bhutan, China, Nepal, Pakistan, where they are gathered from steep rock faces at altitudes between 1000 and 5000 m. It has been

Panchavaktra Ras is a rational combination of Rasadravyas and Kasthaushadhis prescribed in the management of Amavata. Panchavaktra rasa has been taken into consideration for its Pharmaceutical standardization through Standard Operative procedures. In pharmaceutical study, the drug has been prepared in 3 batches adopting Khalviya Rasayana method and physico chemical analysis was carried out on these batches. This formulation was analysed by using Inductively Coupled Plasma with Optical Emission Spectroscopy (ICPOES) and found the major elements Hg, S, B, Fe, Cr, Cu, Ag, C, Pb, As from medicine. The heavy metals like As, Pb and Cd were almost within permissible limits. Organoleptic features of drug are found dark grey astringent taste and pungent odour which is made of the crude drugs within the standard range. The pH of compound was 8.93-8.99 and alkaline in nature. The phytochemical screening showed the presence of Alkaloids, Saponins, Flavanoids, Tanins and Tritepenoids in this drug. The Panchavaktra Ras was subjected to estimation of Microbial contamination which was within normal limits and it does not contain harmful microbes like Enterobacteriacea, E.coli, Salmonella species. Efforts have been made to fix the analytical standards of Panchavaktra ras, which were not found, reported till date.

which has presentation of hearing loss. Hearing impairment is the most frequent sensory deficit in human populations, over 5% of the world ‟s population – 360 million people has disabling hearing loss (328 million adults and 32 million children). 2 Hearing impairment is one such condition that has not much treatment modality. In India itself, 63 million people (6.3%) suffer from significant hearing loss. Sarivadi Vati is an Ayurvedic tablet form which is used in treating hearing problems such as tinnitus, ear infection etc. This formulation having twelve ingredients and two Bhasmas i.e. Abhraka Bhasma, Loha Bhasma. Sarivadi vati is prepared with Bhavana by five Bhavana dravya. SarivadiVati is an Ayurvedic tablet used in treating hearing problems such as tinnitus, ear infection etc.Antimicrobial action of this formulation fights against the bacterial infection in ear. Majority of drugs are having vata kaphashamak action. So, it is more effective in vata kaphaj Karnaroga. Standardization of drug means confirmation of its identity and determination of its quality and purity. The quality assessment of herbal formulations is of paramount importance in order to justify their acceptability in modern system of medicines.

India is blessed with rich flora and minerals, in which some of them have good medicinal properties. Indian traditional practitioners use not only herbs but also minerals for preparing medicines. In ASU medicines metals and minerals processed in herbal juice are used to prepare medicines because it has more potency than herbs which used alone. In this study attempt has been done to standardize the classical Siddha herbo mineral formulation Rathi nagara rasa mezhugu (RNM) through ICP-OES and FTIR techniques. FTIR results revealed that RNM had alcohols, secondary amines, aldehydes, esters and carboxylic acids. ICP-OES analysis showed the presenceof Calcium, Mercury, Iron, Potassium Magnesium, Sodium, Phosphorus and Zinc within the permissible limits. Besides that, the toxic elements Aluminium, Arsenic, Cadmium, Copper, Nickel and Lead were Below Detectable Level.

Rasashastra and bhaishajya kalpana is one of the branch of ayurveda that deals with the pharmaceutical processing, preparation of compound medicines and therapeutic utility of herbal drugs, metals and minerals. Agnikumar Rasa is one such kharaliya herbo-mineral formulation mentioned in various Ayurvedic text. It is primarily used in the management of Agnimandya. According to Ayurveda, Agni has an important role inthe physiological functions of the body. Even there are reference to be used in disease such as Ajirna, Gulma, Visuchika, Grahani, etc. Amongst all formulations termed as Agnikumar rasa have been quoted by most seers and are well acclaimed in the management of agnimandya-janyaroga. Though variation is observed in the description of these kharaliya rasayana and compilation of these will help to study in comparative manner.