heterologous expression in E. coli
Cloning a novel endo-1,4-β-d-glucanase gene from Trichoderma virens and heterologous expression in E. coli
... in E. coli BL21 (DE3) in this ...microbe. Heterologous expression is an efficient route to improve cellulase ...common E. coli as the host cell (Akcapinar et ...otic ...
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Evaluation of different expression systems for the heterologous expression of pyranose 2-oxidase from Trametes multicolor in E. coli
... by heterologous expression in bacterial or yeast systems, is critical for the economic viability of such ...like E. coli are most attractive due to high pro- ductivity, low cost and easy ...
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Increasing the metabolic capacity of Escherichia coli for hydrogen production through heterologous expression of the Ralstonia eutropha SH operon
... malate dehydrogenase in addition to alcohol dehydrogen- ase. Somewhat lower levels of in vitro activity were ob- served with extracts of DJ1, a JW135 derived strain additionally mutated in arcA , and FTGH2, a FTD147 ...
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Physiological relation between respiration activity and heterologous expression of selected benzoylformate decarboxylase variants in Escherichia coli
... Five E. coli SG13009 pKK233-2 clones expressing dif- ferent BFD-variants (Leu476Pro-Ser181Thr, wild type, Leu476His, Leu476Gln and Ser181Thr) were selected for cultivation in modified PanG mineral medium ...
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Heterologous expression of Chorismate synthase from Plasmodium falciparum in Escherichia coli: Optimization of conditions for obtaining soluble and stable recombinant protein
... There are rare codons in PfCS, so to maximize the chance of getting good quality Cs expression in E.coli, C-terminal His-tagged codon optimized genes were synthesized and cloned in pET 28 (a) vector and expressed ...
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Heterologous expression of an acidophilic multicopper oxidase in Escherichia coli and its applications in biorecovery of gold
... the heterologous expression in the pET ...in E. coli can be accomplished by shifting to lower temperature and applied experimental design (Wu et ...
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Bacillus subtilis as potential producer for polyhydroxyalkanoates
... eutropha. Expression of phaC1 from ...for expression of for- eign genes ...with E. coli, the most commonly used host for heterologous expression of genes [34] and it may even be ...
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Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli
... in E. coli cells to reveal their possible functions under stress condi- ...example, heterologous expression of CarDHN from Cerastium arcticum in ...E. coli resulted in increased ...
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Enhanced production of recombinant serratiopeptidase in Escherichia coli and its characterization as a potential biosimilar to native biotherapeutic counterpart
... approaches. Heterologous expression of serratiopeptidase in E. coli was tried before but not found suitable due to the limited yield, and other expression related issues due to its ...
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Improved production of biohydrogen in light-powered Escherichia coli by co-expression of proteorhodopsin and heterologous hydrogenase
... functional heterologous expression of proteorhodopsin in E. coli (Figure ...recombinant E. coli BL21(DE3) harboring pACYC-RDS was cultured, and protein expression was ...
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Heterologous Expression and Characterization of Thermostable Lipases from Geobacillus Thermoleovorans PPD2 Through Escherichia Coli
... The appearance of 43 kDa protein showing lipolytic activity was surprising since the protein did not appeared on crude extract of the control E. coli BL21(DE3)-pET-30a(+). This was only possible when the ...
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Construction of a Shuttle Vector for Heterologous Gene Expression in Escherichia coli and Microalgae Anabaena
... gene expression and (c) neomycin phosphotransferase gene with its own promoter for the selection of transfor- ...and expression of the gfp gene encoding green fluorescent ...the E. coli and ...
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A novel Ffu fusion system for secretory expression of heterologous proteins in Escherichia coli
... in E. coli very ...exploit E. coli for recovering folded disulfide bond-dependent proteins is to instruct the migration of the translated polypeptides to the bac- terial periplasm ...
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Development and application of a transcriptional sensor for detection of heterologous acrylic acid production in E. coli
... mid [17] was amplified with petF2 and petR and the PCR products were intramolecularly ligated to produce attP-PyhcN-eGFP and C3INT-HIS minicircles. A total of 100 ng of C3INT-HIS minicircle and 100 ng attP-PyhcN- eGFP ...
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EXPRESSION OF FULLY FUNCTIONAL GPCR IN E COLI
... bacterial expression of GPCR-family has not yielded functional products 26 ...of E. coli expressed ...for expression revealed that BL21 (DE3) cells produced about ...the expression of ...
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High yield expression of proteins in E coli for NMR studies
... yield expression of heterologous protein in E. coli requires modification of rare codons in the host ac- cording to its usage ...level expression of heterologous protein; a ...
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Expression of Hepatitis B Virus Core Antigen in Native and Fusion Forms in E. coli
... of heterologous sequences to the termini of the HBcAg protein maintain the property of spontaneous self- assembly presenting the foreign peptide on its surface at a high density ...add heterologous ...
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Marine metagenomics: strategies for the discovery of novel enzymes with biotechnological applications from marine environments
... functional expression of metagenomic DNA from psychrophilic marine micro- organisms in heterologous hosts such as ...E. coli may be sub-optimal when the host is cultured at a higher temper- ...
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Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels
... based expression vector in Lemo21 (DE3) cultured in the absence and presence of increasing concentrations of ...the expression vector with the gene encoding secretory BL1 and Lemo21(DE3) harboring an empty ...
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Expression of the Bundle Forming Pilus by Enteropathogenic Escherichia coli Strains of Heterologous Serotypes
... strains for the production of bundlin on the selected media. Among these, 33 (91%) produced BfpA in DMEM (Table 1). No difference was found between DMEM containing 5% fetal bovine serum (a common component of tissue ...
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