high activity binding peptide
Augmentation of the Lipopolysaccharide-Neutralizing Activities of Human Cathelicidin CAP18/LL-37-Derived Antimicrobial Peptides by Replacement with Hydrophobic and Cationic Amino Acid Residues
... augmented activity in binding with and neutralizing a negatively charged amphipathic ...the peptide with the CD14 ...18-mer peptide, thereby exerting augmented LPS-neutralizing ...a ...
11
An N terminal partial sequence of the 13 kDa Pycnopodia helianthoides sperm chemoattractant 'startrak' possesses sperm attracting activity
... attractant peptide, startrak, has been purified and partially identified; the first sperm attractant purified using a behavioral bioassay for sperm ...surface binding assay (Coll et ...sperm-attracting ...
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Synthesis, DNA-Binding and Antibacterial Activity of the Cell-Penetrating Peptide HIV-1 Tat (49-57)
... results from its basic epitope (amino acids 49-57), which is the minimal transduction domain of Tat protein, includes two lysine’s and six arginine’s within a linear sequence of nine amino acid residues (RKKRRQRRR). Tat ...
7
Molecular Imaging of Chemokine Receptor CXCR4
... undesired binding of la- beled T140 to red blood cells, we synthesized the peptide with DOTA or 1,4,7-triazacyclononane- N,N',N''-triacetic acid (NOTA) chelators at the N-terminus, replacing the ...
9
Characterization of a functional thrombin receptor Issues and opportunities
... The cleavage recognition sequence LDPR, thrombin cleavage site, agonist peptide domain, and anion-binding exosite binding domain as defined by structure activity studies 38, 39 with the [r] ...
6
Böhm, Stefanie (2011): The Cdc48 Shp1 complex mediates cell cycle progression by positive regulation of Glc7. Dissertation, LMU München: Fakultät für Biologie
... Mitotic exit is controlled by the spindle position checkpoint. This branch of the spindle checkpoint ensures that the mitotic spindle is oriented along the mother-bud axis and thereby prevents cytokinesis as long as both ...
120
In vitro and in vivo evaluation of novel NGR-modified liposomes containing brucine
... Brucine was purchased from Yuanjian Biopharma Ltd., Co. (Shanghai, People’s Republic of China). The chemical structure of brucine is shown in Figure 1. Internal standard (IS) strychinin was purchased from the Shanghai ...
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High-Throughput Engineering and Analysis of Class II Mhc/Peptide Binding by Yeast Co-Display
... amplification and extension of the degenerate oligonucleotides by PCR such that both ends had at least 50 base pairs (bps) sequence homologous to the linear backbone vector, separately prepared by digesting ptFLUD with ...
185
Characterization of the atrial natriuretic factor system in lungs of the toad Bufo paracnemis
... Molecular cloning has identified three types of natriuretic peptide receptors. Two of these are the guanylyl cyclase ANF receptors, GC-A and GC-B, which are glycoproteins of about 120 kDa forming an integral part ...
7
Human immunodeficiency virus type 1 Nef binds directly to Lck and mitogen-activated protein kinase, inhibiting kinase activity.
... Apart from our current description of Nef interaction with Lck, we have further evidence to suggest that Nef also interacts with the SH3 domain of another src family kinase, Fyn (un- published data). Nef has also been ...
8
Specific serum antibody binding to phosphorylated and non phosphorylated tau in non cognitively impaired, mildly cognitively impaired, and Alzheimer’s disease subjects: an exploratory study
... this binding facilitates tubulin’ s assembly into microtu- bules, permitting neurite extension and stabilization ...its binding to tubulin [3], resulting in decreased axonal transport of vesicles and ...
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Dimerization of a Phage-Display Selected Peptide for Imaging of αvβ6- Integrin: Two Approaches to the Multivalent Effect
... clearly demonstrates the role of the multivalent effect in imaging signal amplification. In addition, the tu- mor PET signal retention of the three divalent probes stayed above 0.70 %ID/g even at 24 h p.i. While the ...
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The involvement of heat shock protein molecular chaperones in MHC class II antigen processing and presentation
... for binding and efficient release of antigen is superceded by the sheer abundance of ...for binding to MHC class II, whether or not a chaperone is available to facilitate antigen transfer or protect antigen ...
216
RNA interference: new mechanistic and biochemical insights with application in oral cancer therapy
... The targeted delivery of a complex composed of CIP2A siRNA, EGFR binding-peptide, and an endosome-disruptive peptide was proven to silence more efficiently the CIP2A oncogene, in vi[r] ...
13
Nonenzymatic glycosylation in vitro and in bovine endothelial cells alters basic fibroblast growth factor activity A model for intracellular glycosylation in diabetes
... In this study, we have evaluated the effect of in vitro nonenzymatic glycosylation on bFGF heparin-binding activity and its mitogenic activity; characterized the effect of high extracell[r] ...
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Streptococcal SspB Peptide Analog Inhibits Saliva Promoted Adhesion and Biofilm Formation of Streptococcus mutans
... SspB peptide analog SspB ...determine peptide interference with biofilm formation, ...gp340 peptide, promoted adherence of ...(A4K-A11K) peptide competitively blocked ...
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Generation of functional CD8+ T Cells by human dendritic cells expressing glypican-3 epitopes
... The yield of each SP6 mMessage Machine reaction was around 20 μg of capped mRNA from 1 μg of linear DNA template. Transcripts were extracted using RNAeasy col- umns and the quality of the mRNA confirmed by dena- turing ...
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Bacterial induction of autoantibodies to β2 glycoprotein I accounts for the infectious etiology of antiphospholipid syndrome
... TLRVYK peptide binding by mouse antipeptide (TLRVYK) Ab’s derived from ...scrambled peptide as a con- trol, is presented in Figure 3 (77% to 89% ...TLRVYK peptide (P > ...scrambled ...
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Functional Implication of an Arg307Gly Substitution in Corticosteroid-Binding Globulin, a Candidate Gene for a Quantitative Trait Locus Associated With Cortisol Variability and Obesity in Pig
... this effect was not significant in the in vitro assays if we average the data from all transfections. However, this effect was significant in two of three transfection assays. Genetic variants of CBG have been reported ...
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Characterization of domains of herpes simplex virus type 1 glycoprotein E involved in Fc binding activity for immunoglobulin G aggregates.
... Three of the fusion proteins containing overlapping gE-1 peptides demonstrated FcR activity; the smallest peptide that retained Fc binding activity includes gE-1 amino acids 183 to 402..[r] ...
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