HILIC–(U)HPLC
Top PDF HILIC–(U)HPLC:
Determination of amino acid without derivatization by using HPLC HILIC column
... The present paper deals with HPLC method development for determination of amino acid. A high performance liquid chromatography (HPLC) method with UV detector is developed for determination of individual 17 ...
9
A HILIC Mechanism Discussion for the Retention of HP β CD
... 1200 HPLC system equipped with both a diode array (DAD, Model G1315D) and refractive index (RI, Model G1362A) detector (Agilent Technologies, Palo Alto, ...USA). HPLC grade acetonitrile, methanol and ...
6
Quantification of potassium chloride in finished dosage forms using hilic with evaporative light scattering detector (elsd)
... HPLC grade Ammonium acetate was purchased from Qualigens Fine Chemicals (Mumbai, India). Acetonitrile, Methanol and Acetone were purchased from Merck Specialties Private Limited (Mumbai, India). HPLC grade ...
7
Comparison of the kinetic performance and retentivity of sub 2μm core shell, hybrid and conventional bare silica phases in hydrophilic interaction chromatography
... HPLC grade acetonitrile (ACN) and tetrahydrofuran (THF) were purchased from Fisher Scientific (Loughborough, UK). Ammonium formate, formic acid, toluene, benzylamine, diphenhydramine, phenylephrine, uracil, ...
31
Development of generic methods for the analysis and purification of polar compounds by high performance liquid chromatography
... during HPLC these forms interconvert in a process known as anomerisation which produces split peaks, making quantitation ...of HILIC columns with excellent peak shape, provided buffers were used in the ...
246
Evaluating the Adsorbed Water Layer on Polar Stationary Phases for Hydrophilic Interaction Chromatography (HILIC)
... All the polar stationary phases selected for this study were either purchased from or kindly donated by the column manufacturers. Table 1 presents the details of all the stationary phases including stationary phase ...
10
Simultaneous untargeted and targeted metabolomics profiling of underivatized primary metabolites in sulfur-deficient barley by ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry
... Many different metabolomics studies in plant biol- ogy are emerging for various aims (e.g. discovering new biocontrol agents, phytomedicine, etc.). Otherwise, in order to understand plant physiological reactions and ...
17
A simple and rapid method for the determination of nicotine in third hand smoke by liquid chromatography and its application for the assessment of contaminated outdoor communal areas
... HILIC separation was performed using a system consisting of an IsoChrom pump (Spectra Physics), with a 150 mm x 4.6 mm, 5 µm Zorbax Carbohydrate Analysis column connected to a 7125 valve manual injector fitted ...
16
EXAMINE THE MASS SPECTROMETRY METHOD FOR TESTING THE EPITRANSCRIPTOME IN CELLULAR LYSATES
... a HILIC method was chosen. Using a Waters BEH Amide HILIC column, we achieved baseline or near baseline separation of many modified nucleotide species in a 17 min ...
10
Urinary metabolomics of young Italian autistic children supports abnormal tryptophan and purine metabolism
... Taking into consideration these methodological issues, in order to maximize the probability of reliably detecting differences in urinary metabolic patterns, we focused on autistic and unrelated typically developing ...
11
Effect of consuming a grape seed supplement with abundant phenolic compounds on the oxidative status of healthy human volunteers
... the other catechin-containing derivatives. The natural fluorescence of the nongalloylated flavan-3-ols was greater, so the procyanidin tetramer, [EC-EC-EC-EC], was quantified whereas the other two procyanidin oligo- ...
8
Accurate and efficient amino acid analysis for protein quantification using hydrophilic interaction chromatography coupled tandem mass spectrometry
... amino acids (Fig. 1a) were resolved chromatographically; through a solvent gradient profile that included initiat- ing runs with 100% solvent B (0.25 mL/min), transition- ing to 10% solvent A within 2 min, then to 50% ...
12
Contribution to the Method of Sugar Analysis in Legume Grains for Ensiling - A Pilot Study
... and HPLC. Following HPLC, either glucose, fructose and sucrose (HPLC-1) or these monomers plus galactose (HPLC-2) or HPLC-2 plus oligomeric carbohydrates (raffinose, stachyose, ...
7
Optimization of a micro-high-performance liquid chromatography method for determination of metronidazole benzoate in their standard powder and in dosage pharmaceuticals
... functions of cinematography workstation, and the technical criteria make it leading instruments with excellent stability and reliability. The LC100-type HPLC-UV consists of a double-beam optical spectrometer ...
12
DEVELOPMENT AND VALIDATION OF U V SPECTROPHOTOMETRIC METHOD AND RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF OLANZAPINE AND SERTRALINE HYDROCHLORIDE IN BULK AND SYNTHETIC MIXTURE
... The results of present study indicate that the proposed UV spectroscopic and RP- HPLC method is simple, rapid, and precise and accurate. Statistical analysis proves that the method is repeatable and selective for ...
11
Method Development and Validation for Simultaneous Estimation of Aceclofenac, Paracetamol & Chlorzoxazone in Tablets by HPLC, HPTLC & Aceclofenac By Differential Spectroscopy.
... The separation of aceclofenac, paracetamol and chlorzoxazone by HPLC method has been carried out in two ways. The first method was performed without being using the internal standard while the second method has ...
112
PHYSICO CHEMICAL AND HPLC ANALYSIS OF THE WHOLE PLANT OF ACHYRANTHES ASPERA (LINN) OBTAINED FROM VINDHYA REGION OF U P INDIA
... Amaranthaceae is a cosmopolitan family consisting of 64 genera and about 800 species, mostly abundant in tropical regions of America, Africa, and India .The family represented by herbs and few shrubs, contains most of ...
10
Practical observations on the performance of bare silica in hydrophilic interaction compared with C18 reversed phase liquid chromatography
... of HILIC compared with ...in HILIC for basic compounds, due to the lower viscosity of the mobile phases used and resul- tant enhanced solute diffusivity, and that also longer columns were applicable ...
9
Use of hemicellulase in sequence with hydrogen peroxide and laccase for improvement of teak veneer surface color
... ) were also calculated to compare and determine suitable conditions. Furthermore, the solution samples obtained from veneer treatments were also ana- lyzed for sugar composition using high performance liquid ...
5
Determination of cannabinoids in Cannabis sativa L. samples for recreational, medical, and forensic purposes by reversed-phase liquid chromatography-ultraviolet detection
... cannabigerol, cannabidiol, cannabinol, Δ 9-tetrahydrocannabinol, and tetrahydrocannabinolic acid within 10 min. For all target analytes, the %-Bias at the lower and upper calibration range varied from − 1.3 to 10.3% and ...
10