Histone methyltransferase (HMT)
Position-specific intron retention is mediated by the histone methyltransferase SDG725
... Several lines of evidence support the notion that H3K36me2 in rice probably serves as the functional coun- terpart of H3K36me3 in animal cells [4, 16, 17]. H3K36me2 in plants and H3K36me3 in animals share similar ...
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Targeting histone methyltransferase and demethylase in acute myeloid leukemia therapy
... or histone methylation and demethylation (EZH2, MLL, DOT1L) are frequently mutated in primary and secondary ...some histone demethylases, such as LSD1, are frequently overexpressed in ...the histone ...
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Histone demethylase KDM2B upregulates histone methyltransferase EZH2 expression and contributes to the progression of ovarian cancer in vitro and in vivo
... The increased expression of KDM2B in ovarian cancer was associated with histological type, tumor grade, FIGO stage, and lymph node metastasis, and it was positively correlated with EZH2. KDM2B depletion reduced the ...
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Genomic Disruption of the Histone Methyltransferase SETD2 in Chronic Lymphocytic Leukemia
... on histone H3 (H3K36me3), 31,32 a mark that is associated with actively tran- scribed regions and is involved in transcriptional elongation and ...epigenetic histone mark to other important cellular ...
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Histone methyltransferase SMYD2: ubiquitous regulator of disease
... Similarly, in muscle, SMYD2 methylates Hsp90 to main- tain the stability of titin and muscle function (Fig. 3a) [54]. This discrepancy in heart development between mice and zebrafish may be due to the following reasons: ...
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The histone methyltransferase Setd7 promotes pancreatic progenitor identity
... differentiate into definitive endoderm (DE) and PaE (Fig. 5D). ChIP analysis showed increased RNA polymerase II (PoI II) binding at the promoter regions of Foxa2, Eomes and Pdx1 in PaE differentiated cells compared with ...
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Molecular function and regulation of the histone methyltransferase, Ezh2
... EZH2 (Enhancer of zeste 2) is an important developmental regulator that plays an important role in numerous biological processes including multi-cellular development, stem cell biology, and cancer development. As an ...
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MES 4: an autosome associated histone methyltransferase that participates in silencing the X chromosomes in the C elegans germ line
... We used antibodies specific for H3 and H4 peptides containing methylated lysines to investigate the residue specificity of MES- 4. Mouse NSD1 has been reported to dimethylate H3K36 and H4K20 (Rayasam et al., 2003). Based ...
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Oncogenic roles of the SETDB2 histone methyltransferase in gastric cancer
... the HMT genes in various cancers [21–24]. The H3K9 tri-methyltransferase genes, SETDB1 and SUV39H1, have been shown to be overexpressed in cancers, such as lung and liver cancers ...
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PubMedCentral-PMC5072726.pdf
... the methyltransferase activity of EZH2 is required for RelB transcription in ...blocked histone methyltransferase activity of EZH2 as detected by a decrease in H3K27me3 ...The histone ...
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Germinal center dysregulation by histone methyltransferase EZH2 promotes lymphomagenesis
... The histone H3 lysine 27 methyltransferase enhancer of zeste homolog 2 (EZH2) is highly expressed in GC B cells and is often constitutively activated in GC-derived non-Hodgkin lymphomas ...
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Histone methyltransferase PRMT6 plays an oncogenic role of in prostate cancer
... Protein extraction and western blot analysis Protein was extracted from whole-cell lysates using Kinexus Lysis Buffer with Lysis Buffer Cocktail (Kinexus Bioinformatics Corporation, Vancouver, British Columbia, Canada) ...
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Structure and catalytic mechanism of the human histone methyltransferase SET7/9
... methyl Lys 4 peptide as a substrate for further methylation. Thus we have further analysed the methylation reaction. Reaction mixtures were set up with unmodified peptide and AdoMet, and conditions found where the ...
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TALEN Mediated FLAG Tagging of Endogenous Histone Methyltransferase DOT1L
... Histone modification including H3 lysine 79 methylation (H3K79me) plays a key role during gene transcription and DNA damage repair. DOT1L, the sole methyltransferase for three states of H3K79me, is ...
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Conservation and function of the histone methyltransferase Set2
... While H3K36 methylation has been demonstrated in budding yeast, its presence and relative abundance in other organisms has not been well established. To determine the conservation and relative abundance of H3K36 ...
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Methylation of Histone H3 Lysine 36 Is Required for Normal Development in Neurospora crassa
... Histone methyltransferase assays. A 1.1-kb fragment coding for the first 372 amino acids of N. crassa SET-2 was amplified from DNA pools of an N. crassa conidial cDNA library (31) with primers SET2–20 and ...
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Investigating the Failure of Myogenesis in the Pediatric Tumor Rhabdomyosarcoma
... Indeed, in agreement with this model, recent publications have demonstrated that downregulation of a histone methyltransferase associated with inhibition (Lee et al., 2011), interferen[r] ...
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Dot1l expression predicts adverse postoperative prognosis of patients with clear-cell renal cell carcinoma
... Disruptor of telomeric silencing 1-like (Dot1l), located on the nucleosome surface, is the only known mammalian histone methyltransferase which targets the H3K79 position [10]. Just like its homolog gene ...
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DNA Replication Origin Function Is Promoted by H3K4 Di-methylation in Saccharomyces cerevisiae
... Set1 histone methyltransferase and the Bre1 E3 ubiquitin ligase have well-established roles in regulating gene expression (Shukla et ...Rpd3 histone deacetylase that regulates origin firing time ...
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Effect of heat moisture treatment on multi scale structures and physicochemical properties of breadfruit starch
... suggested HMT-induced realignment of chains. Compared with native breadfruit starch, the modified starches possessed a slight decrease in the average repeat distance (d) of semicrystalline lamellae. The ...
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