HLA-specific antibody detection using Luminex technology
Development of a Sensitive and Specific Serological Assay Based on Luminex Technology for Detection of Antibodies to Zaire Ebola Virus
... in using whole viral lysates in ELISA and similar methods is the potential number of false-positive samples, as observed in the early ages of HIV diagnosis, specifically in Africa, where extraordinarily high HIV ...
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Novel method for simultaneously detecting HPA and HLA antibodies using Luminex microbeads
... the Luminex assay in this study could improve the sensitivity for the detection of the HPA antibodies and is also rapid with results avail- able in about 3 ...ing HLA and multi-type of platelet ...
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Complement-dependent cytotoxicity and Luminex technology for human leucocyte antigen antibody detection in kidney transplant candidates exposed to different sensitizing events
... new antibody detection methods were intro- duced, including flow cytometry (FC) and solid phase assay (SPA)–enzyme-linked immunosorbent assay (ELISA) and Luminex ...purified HLA molecules ...
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Cytomegalovirus glycoprotein B-specific antibody analysis using electrochemiluminescence detection-based techniques.
... ECL technology for determination of CMV gB-specific anti- bodies in biologically relevant ...the detection of recombinant protein immobilized to microtiter plates (data not ...high antibody ...
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Development of a sensitive Luminex xMAP based microsphere immunoassay for specific detection of Iris yellow spot virus
... Five specific MAbs (16D9, 11C6, 7F4, 12C10, and 14H12) were identified and used for developing the Luminex xMAP-based MIA systems along with a polyclonal antibody against ...of Luminex ...
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Species Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by Use of Luminex xMAP Technology
... throughput Luminex microbead hybridization ...the detection of each fungal ...panel using the Luminex ...the Luminex-based technology presented permits rapid and reliable ...
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Intraneuronal Aβ detection in 5xFAD mice by a new Aβ-specific antibody
... As previously described [58,59], female BALB/c mice were immunized with O-Ab42 produced as outlined above. For the initial injection, the immunogen was suspended in 200 μ l Complete Freund ’ s Adjuvant at a concentration ...
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Detection of HLA class I-specific antibodies by the QuikScreen enzyme-linked immunosorbent assay.
... of antibody specific for antigens that had frequencies of ,1% in the ...tests using a mixture of lymphocytes from different subjects, found that reactivity with one individual could be detected with ...
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Development, characterisation and application of monoclonal antibodies for the detection and quantification of infectious salmon anaemia virus in plasma samples using luminex bead array technology
... Bio-Plex technology (BioRad based on Luminex xMAP technology) is a bead-based technol- ogy that is widely used in human health and is being developed for veterinary medicine [9] as it allows the ...
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Development and Performance Evaluation of an Antibody-Based Technology for Detection of E. coli O157 in Meat Samples and Its Potential Evolution Using Antibody Engineering
... that using the lowest CGC concentration assessed was optimal to further optimize the concentrations of the mAb and pAb-b because it gave a clear positive read out with ...
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Tracking the amphibian pathogens Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans by using a highly specific monoclonal antibody and lateral-flow technology
... bound antibody visualized by incubating wells with tetramethyl benzidine (TMB: T2885; Sigma) substrate solution for 30 min, after which reactions were stopped by the addition of 3 M H 2 SO 4 ...nm using a ...
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First report on the antibody verification of HLA-ABC epitopes recorded in the website-based HLA Epitope Registry
... 95 antibody-verified HLA-ABC epitopes including 33 defined by combinations of amino acid config- ...an antibody-defined epitope depends on the antibody reactivity pattern with an informative ...
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Characterization of the Anti-HLA Class I and II IgG Antibodies in Moroccan IVIg Using Regular Beads and Ibeads in Luminex Multiplex Single Antigen Immunoassay
... primary specific objective of this investigation is to characterize IgG reactivity against HLA-I and HLA-II in Moroccan ...by HLA antibody ...polyclonal antibody strength may ...
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Direct quantitative measurement of the kinetics of HLA specific antibody interactions with isolated HLA proteins
... monoclonal, HLA-specific ...different HLA antibody/HLA allele combinations, in the order of ...expected HLA specificity and we can discern a distinct signal for low nanomolar ...
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Predictive Value of Donor Specific Antibody Measured by Luminex Single Antigen Assay for Antibody Mediated Rejection after Kidney Transplantation
... 그러나 Luminex-PRA는 ELISA-PRA에 비해 민감도가 높으므로 임상적으로 거부반응을 유발하지 않는 강도가 매우 약한 항체를 위양성으로 보고할 가능성이 ...분석한 Luminex-PRA 검 사 결과는 ELISA-PRA에 비해 민감도가 높은 대신 제공자 비특이 항체도 검출하므로 특이도가 ...
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Detection of Salmonella-specific antibody in swine oral fluids
... Salmonella detection are laborious and ...through detection of Salmonella-specific serum antibody is a reliable method, but presents labor and animal-welfare ...
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Development of specific antibody fragments for the detection and treatment of melanoma
... Various staining methods including the streptavidin-biotin and the streptavidin-HRP methods have been used with poor results. We were recently able to detect whole Mab LHM2 (that targets the same epitope as RAFTS and ...
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Efficacy of plasmapheresis on donor‐specific antibody reduction by HLA specificity in post–kidney transplant recipients
... The phenomenon of antibody rebound after TPE is well described. Dau 4 reported a significant increase in B-cell percentage in peripheral blood lymphocyte popu- lations with TPE, which leads to increasing ...
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Targeting melanoma using specific antibody fragments
... produced affinity increases of up to 1000-fold (Marks et al., 1992; Low et a l, 1996). This technique and chain shuffling share the advantage over site-directed mutagenesis of allowing variations in non-CDR regions of ...
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Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
... 1. Introduction During the last decades, biosensors have emerged as essential tools in biomedical applications, offering exceptional advantages over conventional clinical techniques for diagnostics and therapy ...
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