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Human bronchial epithelial cells

Theophylline action on primary human bronchial epithelial cells under proinflammatory stimuli and steroidal drugs: a therapeutic rationale approach

Theophylline action on primary human bronchial epithelial cells under proinflammatory stimuli and steroidal drugs: a therapeutic rationale approach

... Notes: Theophylline is not present. Protein levels were determined by Western blot analysis. The gray level of every band was measured to check the difference in the protein expressions of hBecs by imageJ software. Data ...

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Gene and protein expression of tlr 9 with lipopolysaccharide in human bronchial epithelial cells

Gene and protein expression of tlr 9 with lipopolysaccharide in human bronchial epithelial cells

... media. Human bronchial epithelial cells (HBEC) were cultured in the T75 flask with the media ...The cells were kept in the incubator so as to allow the growth and avoid the ...The ...

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Nicotine signals through muscle-type and neuronal nicotinic acetylcholine receptors in both human bronchial epithelial cells and airway fibroblasts

Nicotine signals through muscle-type and neuronal nicotinic acetylcholine receptors in both human bronchial epithelial cells and airway fibroblasts

... normal human bronchial epithelial cells from a number of different human donors consist- ently express functional nAChR and that these cell types differ in the type of nAChR they ...HBE ...

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Human H7N9 virus induces a more pronounced pro inflammatory cytokine but an attenuated interferon response in human bronchial epithelial cells when compared with an epidemiologically linked chicken H7N9 virus

Human H7N9 virus induces a more pronounced pro inflammatory cytokine but an attenuated interferon response in human bronchial epithelial cells when compared with an epidemiologically linked chicken H7N9 virus

... the human H7N9 viruses were higher than those of phylogenetically unrelated duck H7N9 virus and the 2009 pandemic H1N1 virus in differentiated normal human bronchial epithelial cells ...

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Anti-inflammatory effects of antibacterials on human bronchial epithelial cells

Anti-inflammatory effects of antibacterials on human bronchial epithelial cells

... immune cells, NHBE cells (normal human bronchial epithelial cells) and immortalised respiratory cell lines ...in cells of the immune ...

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Bilateral Entry and Release of Middle East Respiratory Syndrome Coronavirus Induces Profound Apoptosis of Human Bronchial Epithelial Cells

Bilateral Entry and Release of Middle East Respiratory Syndrome Coronavirus Induces Profound Apoptosis of Human Bronchial Epithelial Cells

... novel human coronavirus (CoV) causing acute pneumonia with renal failure emerged in the Middle ...utilizes human angiotensin-converting enzyme 2 (hACE2) as the func- tional receptor (5–7), MERS-CoV exploits ...

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Simultaneous Treatment of Human Bronchial Epithelial Cells with Serine and Cysteine Protease Inhibitors Prevents Severe Acute Respiratory Syndrome Coronavirus Entry

Simultaneous Treatment of Human Bronchial Epithelial Cells with Serine and Cysteine Protease Inhibitors Prevents Severe Acute Respiratory Syndrome Coronavirus Entry

... in cells expressing TMPRSS2 (19, ...enter cells FIG 9 Effect of TMPRSS2 and HAT siRNA-mediated knockdown on SARS-CoV entry into Calu-3 ...The cells were transiently transfected with siRNAs targeting ...

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TWEAK enhances TGF-β-induced epithelial-mesenchymal transition in human bronchial epithelial cells

TWEAK enhances TGF-β-induced epithelial-mesenchymal transition in human bronchial epithelial cells

... from bronchial epithelial cells using the RNeasy plus mini kit (Qiagen, Valencia, CA, USA) with DNase treatment, followed by cDNA synthesis using the First-Strand cDNA Synthesis kit (GE Healthcare, ...

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Cholesterol-Rich Microdomains as Docking Platforms for Respiratory Syncytial Virus in Normal Human Bronchial Epithelial Cells

Cholesterol-Rich Microdomains as Docking Platforms for Respiratory Syncytial Virus in Normal Human Bronchial Epithelial Cells

... HeLa cells without affecting viral attachment and suggested that fusion, the only remaining step in entry, must be the target for this ...NHBE cells begin at the plasma ...NHBE cells grown on 6-well ...

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Mechanism of cigarette smoke condensate-induced acute inflammatory response in human bronchial epithelial cells

Mechanism of cigarette smoke condensate-induced acute inflammatory response in human bronchial epithelial cells

... RPMI 1640, penicillin, streptomycin, fungizone, and trypsin/EDTA were obtained from Cellgro (Herndon, VA, USA). Fetal bovine serum was from Atlanta Biochemicals (Atlanta, GA, USA). The culture medium (BEGM), growth ...

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Modulation of airway inflammation in cystic fibrosis  In vivo suppression of interleukin 8 levels on the respiratory epithelial surface by aerosolization of recombinant secretory leukoprotease inhibitor

Modulation of airway inflammation in cystic fibrosis In vivo suppression of interleukin 8 levels on the respiratory epithelial surface by aerosolization of recombinant secretory leukoprotease inhibitor

... rSLPI. Human bronchial epithelial cells exposed to CF ELF recovered before rSLPI therapy expressed IL-8 mRNA transcripts, but ELF recovered after rSLPI therapy induced far less ...

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Ligation of protease activated receptor 1 enhances αv
               β6
               integrin–dependent TGF β activation and promotes acute lung injury

Ligation of protease activated receptor 1 enhances αv β6 integrin–dependent TGF β activation and promotes acute lung injury

... airway epithelial (normal human bronchial epithelial) cells were obtained commercially from Cambrex, and the TML epithelial reporter cells stably expressing firefly ...

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CD147 increases mucus secretion induced by cigarette smoke in COPD

CD147 increases mucus secretion induced by cigarette smoke in COPD

... of epithelial cells ...airway epithelial mucin production ...in human bronchial epithelial cells, which was in agree- ment with the results from lung ...

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Fibroblast-epithelial cell interactions drive epithelial-mesenchymal transition differently in cells from normal and COPD patients

Fibroblast-epithelial cell interactions drive epithelial-mesenchymal transition differently in cells from normal and COPD patients

... in bronchial epithelial cells derived from COPD subjects, demonstrating that these cells are undergoing EMT ...normal bronchial epithelial cells responded to cell culture ...

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Regional heterogeneity in response of airway epithelial cells to cigarette smoke

Regional heterogeneity in response of airway epithelial cells to cigarette smoke

... airway epithelial cells from different sites in the lungs have not been fully ...Methods: Human small airway epithelial cells (SAECs) and normal human bronchial ...

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S100A7/psoriasin expression in the human lung: unchanged in patients with COPD, but upregulated upon positive S. aureusdetection

S100A7/psoriasin expression in the human lung: unchanged in patients with COPD, but upregulated upon positive S. aureusdetection

... the human skin, is constitutively expressed in perinuclear granules of human bronchial epithelial cells and alveolar ...by epithelial cells, S100A7/psoriasin mRNA ...

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A(H7N9) Virus Results in Early Induction of Proinflammatory Cytokine Responses in both Human Lung Epithelial and Endothelial Cells and Shows Increased Human Adaptation Compared with Avian H5N1 Virus

A(H7N9) Virus Results in Early Induction of Proinflammatory Cytokine Responses in both Human Lung Epithelial and Endothelial Cells and Shows Increased Human Adaptation Compared with Avian H5N1 Virus

... the human respiratory tract, including the trachea, bronchus, and lung (9, 10, ...primary bronchial and tracheal epithelial cells, targeting both ciliated and mucin-secretory cells, ...

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Rhinovirus Activates Interleukin-8 Expression via a Src/p110β Phosphatidylinositol 3-Kinase/Akt Pathway in Human Airway Epithelial Cells

Rhinovirus Activates Interleukin-8 Expression via a Src/p110β Phosphatidylinositol 3-Kinase/Akt Pathway in Human Airway Epithelial Cells

... of human bronchial epithelial cells with RV39 induces rapid activation of PI 3-ki- nase and phosphorylation of the PI 3-kinase p85 ␣ regulatory subunit ...⫺ human bronchial ...

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Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levels

Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levels

... nasal epithelial cells in smokers ...in human bronchial epithelial cells and that the expression of the CFTR protein is also reduced in bronchial epithelium of patients ...

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Interleukin-26 Production in Human Primary Bronchial Epithelial Cells in Response to Viral Stimulation: Modulation by Th17 cytokines

Interleukin-26 Production in Human Primary Bronchial Epithelial Cells in Response to Viral Stimulation: Modulation by Th17 cytokines

... transformed human bronchial epithelial cells via MAP kinases ...primary bronchial epithelial cells occurs via MAP kinases and, therefore, their simultaneous stimula- tion ...

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