Human fibroblast-like synoviocytes of rheumatoid arthritis
Different expression of β subunits of the KCa1 1 channel by invasive and non invasive human fibroblast like synoviocytes
... FLS: fibroblast-like synoviocyte(s); GAPDH: glyceraldehyde 3-phosphate dehydrogenase; IgG: immunoglobulin G; IRB: institutional review board; KCa: calcium- activated potassium channel; LCA: lithocholic ...
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Anti inflammatory and antiarthritic effects of piperine in human interleukin 1β stimulated fibroblast like synoviocytes and in rat arthritis models
... Black pepper (Piper nigrum) is commonly used as a spice in human diets, but it is also used as a medicine, a preservative, and a perfume in many Asian countries. An extract of the active phenolic component, ...
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Effects of inflammatory cytokine IL 27 on the activation of fibroblast like synoviocytes in rheumatoid arthritis
... FLS. Human FLS constitutively expressed functional IL-27 receptor com- plex WSX-1 and gp130, and STAT1 was phosphorylated upon IL-27 stimulation (Figures 1 and ...in human monocytes and keratinocytes ...
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Original Article Deoxycytidine kinase promotes the migration and invasion of fibroblast-like synoviocytes from rheumatoid arthritis patients
... Abstract: Rheumatoid arthritis (RA) is a complex, multi-system disease whose primary site of inflammatory tissue damage is the ...RA fibroblast-like synoviocytes (FLS) play a criti- cal ...
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Glucose 6 phosphate isomerase promotes the proliferation and inhibits the apoptosis in fibroblast like synoviocytes in rheumatoid arthritis
... inflammatory arthritis mouse model [17]. Furthermore, recombinant human GPI was shown to have the ability to induce chronic arthritis in the mice, which was major histocompatibility complex (MHC) ...
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MLN51and GM CSF involvement in the proliferation of fibroblast like synoviocytes in the pathogenesis of rheumatoid arthritis
... and MI380 microarray chips, consisting of 384 human and mouse cDNA clones, respectively. Total RNA (20 μ g) was reverse-transcribed in the presence of Cy-3-conjugated or Cy- 5-conjugated dUTP (Amersham Pharmacia ...
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Innate immunity triggers IL 32 expression by fibroblast like synoviocytes in rheumatoid arthritis
... Human FLSs were isolated from synovial tissues from four different RA and OA (osteoarthritis) patients at the time of knee-joint arthroscopic synovectomy, as described previously [19]. The diagnosis conformed to ...
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Diosgenin, a plant steroid, induces apoptosis in human rheumatoid arthritis synoviocytes with cyclooxygenase 2 overexpression
... including rheumatoid synovium and in cultured human RA fibroblast-like synovio- cytes (FLS) ...in rheumatoid synovial explants or in cultured rheu- matoid ...
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Apoptosis is not the major death mechanism induced by celecoxib on rheumatoid arthritis synovial fibroblasts
... Celecoxib does not induce caspase activation in rheumatoid arthritis fibroblast-like synoviocytes (RA FLSs). (a) FLSs were stimulated for 2 hours with celecoxib at indicated ...
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The GTPase Rac Regulates the Proliferation and Invasion of Fibroblast-Like Synoviocytes from Rheumatoid Arthritis Patients
... the GDP-bound form. One day before the assay, RA-FLS was serum starved with or without 50 μM NSC23766. After 24 h of treatment, cells were stimulated with IL-1β for 5 min and subsequently lysed with lysis buffer. ...
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Long noncoding RNA expression profile in fibroblast like synoviocytes from patients with rheumatoid arthritis
... Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology). Briefly, RNA was purified using the RNeasy Mini Kit ...
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Fibroblast activation protein is expressed by rheumatoid myofibroblast like synoviocytes
... in human have been detected only in granulation tissue of healing wounds [25], desmoplas- tic reactions [26], and the reactive stroma of malignant tissue ...of arthritis was ...
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A novel function of artesunate on inhibiting migration and invasion of fibroblast like synoviocytes from rheumatoid arthritis patients
... collagen-induced arthritis model in rats — suggesting it may be a potent drug for rheumatoid arthritis (RA) ...of fibroblast-like synoviocytes (FLS) from patients with ...
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Endoplasmic reticulum stress cooperates with Toll like receptor ligation in driving activation of rheumatoid arthritis fibroblast like synoviocytes
... Our inability to observe a similar effect of ER stress on mRNA stability in macrophages was surprising, given the available literature. For example, the IRE1α- XBP1 signaling pathway was shown to be a critical element of ...
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ADAM 17 is expressed on rheumatoid arthritis fibroblast like synoviocytes and regulates proinflammatory mediator expression and monocyte adhesion
... For analysis of ADAM-17 expression in RA STs and RA FLSs, rabbit anti-ADAM-17 was used as the primary antibody. (High-activity STs were selected from RA STs using hematoxylin and eosin stain.) (To measure the secretion ...
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RHAMM induces progression of rheumatoid arthritis by enhancing the functions of fibroblast like synoviocytes
... RAFs produce IL-6 and IL-8, which are pro-inflammatory cytokines that play crucial roles in the pathophysiology of RA, and contribute to the inflamma- tion and joint damage [28 – 32]. These inflammatory cy- tokines have ...
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Effect of 1,25 (OH)2D3 on proliferation of fibroblast like synoviocytes and expressions of pro inflammatory cytokines through regulating MicroRNA 22 in a rat model of rheumatoid arthritis
... Rheumatoid arthritis (RA), affecting ...unknown, fibroblast- like synoviocytes (FLS) have been reported to play a critical role in the pathogenesis of RA [3, ...controlling human ...
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Chemerin activates fibroblast like synoviocytes in patients with rheumatoid arthritis
... To detect phosphorylated and total ERK1/2, p38MAPK, c-Jun N-terminal kinase (JNK)1/2 or Akt, cells were incu- bated in a medium supplemented with 10 nM human recombinant chemerin prior to lysis for 5, 15 or 30 ...
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Glutaminase 1 plays a key role in the cell growth of fibroblast like synoviocytes in rheumatoid arthritis
... the rheumatoid synovium and contributes to RA pathogenicity by inducing RANKL ...In human neurons, GLS1 expression was upregu- lated by IL-1β or TNF-α treatment, and in a rat hepatoma model IL-6 or TNF-α ...
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Adiponectin may contribute to synovitis and joint destruction in rheumatoid arthritis by stimulating vascular endothelial growth factor, matrix metalloproteinase 1, and matrix metalloproteinase 13 expression in fibroblast like synoviocytes more than proinflammatory mediators
... is a single nonglycosylated polypeptide chain produced in Escherichia coli with a molecular weight of 25.1 kDa and con- taining 231 amino acids. Thus, the adiponectin used in this system may not completely duplicate the ...
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