Human peripheral blood CD34 + cells
Engraftment Engraftment kinetics of human CD34 þ cells from cord blood and mobilized peripheral blood co-transplanted into NOD/SCID mice
... CB cells) resulted in almost complete abrogation of CB- engraftment (Figure 1b, cell ratio 50: ...stem cells, lower than in the initial experiments because of the limited availability of these cells, ...
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Replication of human cytomegalovirus in human peripheral blood T cells.
... To prove the ability of CTC to replicate HCMV, PHA-stimulated T cells that had been held in IL-2 for 10 days were infected with HCMV strain AD169 at an MOI of 5.. All of these T-cell cul[r] ...
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Genomic imprinting in human stem cells and human peripheral blood leukocytes
... Both human and mouse embryonic stem cells have been shown to lack normal imprinted expression of some genes, expressing certain transcripts ...ES cells, like all other cell lines, are grown in ...
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Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation
... mechanism is based on intracellular-ice formation by the reduction temperature of water in the cells without any cryogenic damages. This could effectively stop cell movement and shut down the cell biochemical ...
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Hyaline Cartilage Regeneration on Osteochondral Defects by Intraarticular Injection of Human Peripheral Blood CD34+ Stem Cells, Hyaluronic Acid and Growth Factor In a Rat Model
... of human peripheral blood CD 34+ stem ...34+ cells have plasticity and can form chondrocytes which further trigger hyaline cartilage ...stem cells developed into chondrocytes was not ...
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Isolation of human peripheral blood mononuclear cells (PBMCs), PBMC subsets
... from human biopsies Human intestinal biopsies from IBD patients were obtained during colonoscopy following informed written consent according to the approval of the Ethics Committee of the Friedrich ...
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Surface immunoglobulin on activated human peripheral blood thymus derived cells
... activated human lymphocytes may differ for different ...large cells which had the T markers, and it cannot be used to identify a lymphocyte as a bone marrow-derived (B) ...(T) cells ought to be ...
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Definition of a subset of human peripheral blood mononuclear cells that are permissive to human cytomegalovirus infection.
... We have tested the capacity of HCMV to in vitro infect human peripheral blood mononuclear cells (PBMC) from healthy donors and found that certain PBMC are permissive to HCMV infection.. [r] ...
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VPAC1 receptor expression in peripheral blood mononuclear cells in a human endotoxemia model
... The VPAC1 receptor expression profile was not consis- tent across leukocyte subtypes. Given that leukocyte count was significantly increased by LPS, the absolute reduction in monocyte and lymphocyte count indicates that ...
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Peripheral blood derived mononuclear cells enhance osteoarthritic human chondrocyte migration
... environments, cells often navigate in the context of multiple simultaneously presented cues where the ECM environment adds further complexity that could provide conflicting signals to migrating cells if it ...
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Isolation and Characterization of Multipotent and Pluripotent Stem Cells from Human Peripheral Blood
... conclude, blood reveals to be a critical site where different types of stem cell population with multipotent and pluripotent capabilities can be found, representing an extremely useful source for clinical ...the ...
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CD34+ CD45- Cells Isolated from Human Blood Have Molecular and Functional Characteristics of Vascular Endothelial Progenitors
... progenitor cells remain a matter of ...CD45- CD34/133+ cell population in human blood, we purified these cells using elutriation, immunomagnetic selection and FACS sorting from both ...
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Optimization of methods for peripheral blood mononuclear cells isolation and expansion of human gamma delta T cells
... Peripheral blood mononuclear cells Isolation: A volume of 5 ml fresh blood was drawn using the method stated ...added. Blood from the syringe was transferred to this tube and tilting ...
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Radiosensitivity of peripheral blood B cells in pigs
... matic cells have traditionally been in the spotlight and many labs have investigated their biodosimetric ...of human peripheral blood lym- phocytes (CD8 + NK cells, CD21 – and CD27 + B ...
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Analysis of the microRNA transcriptome and expression of different isomiRs in human peripheral blood mononuclear cells
... Novel miRNA identification The reads that did not match with the annotated se- quence databases and that mapped to intergenic and in- tronic regions of the human genome were checked to see if these were potential ...
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Optimal conditions for recovery of the human immunodeficiency virus from peripheral blood mononuclear cells
... The culture vessels containing 1.5 x 106 to 3 x 106 cells per ml at a surface cell density of 0.3 x 106 to 0.75 x 106/cm2 generally yielded higher levels of RT activity and in the shorte[r] ...
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Infection of human peripheral blood mononuclear cells with a temperature-sensitive mutant of measles virus.
... Because the in vitro infection of stimulated PBMCs leads to death of productively infected cells, we have studied MV-induced immunosuppression in nondestructive condi- tions.. Using a te[r] ...
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The Effect of CCCP on Mitophagy in CD4 CD25 Regulatory T Cells in Human Peripheral Blood
... The cells in each group were collected, centrifuged, and the supernatant was removed, add 1 ml of pre-heat mitochondrial probe working solution and 1 ml of pre-heat lysosome probe working solution to the healthy ...
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Microplate hybridization for Borna disease virus RNA in human peripheral blood mononuclear cells.
... The microplate hybridization we developed is simple and sensitive enough to detect BDV p24 cDNA in PBMCs derived from patients and blood donors. Although the hypothesis that there is an association of BDV with ...
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Comparative Analysis of the Regulatory T Cells Dynamics in Peripheral Blood in Human and Porcine Polytrauma
... T cells (Treg) play a key role in the suppression of the immune response and in the maintenance of immuno- logical ...in blood after trauma, and nothing is known about Treg in the porcine polytrauma ...
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