Human peripheral blood mononuclear cells (PBMCs)
Impaired Toll like receptor 2 mediated Th1 and Th17/22 cytokines secretion in human peripheral blood mononuclear cells from patients with atopic dermatitis
... from PBMCs was almost abolished in AD ...in PBMCs at basal level or upon stimulation by other ligands including lipopolysaccha- ride (LPS), Staphylococcal enterotoxin B (SEB), house dust mite (HDM), Candida ...
10
Sex-associated differences in mitochondrial function in human peripheral blood mononuclear cells (PBMCs) and brain
... in human peripheral tissues, especially fibroblasts and blood cells (primarily platelets and lymphocytes) indicate a relation be- tween peripheral and cerebral parameters, including ...
10
Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation
... diluted blood samples were gently layered and isolated by density centrifugation on Ficoll-Hypaque® gradients (Sigma- Aldrich, UK) using two different centrifugation programs at room temperature (20 minutes at 700 ...
6
Leukotriene B4 activates intracellular calcium and augments human osteoclastogenesis
... whole blood ? derived human peripheral blood mononuclear cells (PBMCs), differentiated them towards an osteoclast lineage and then performed immunofluorescence and ...
12
1-s2.0-S1097276518303575-main.pdf
... HIV-1-infected cells, we infected THP1 cells or human peripheral blood mononuclear cells (PBMCs) with wild-type and Vpr-deficient mutant HIV-1 and examined the ...
15
s41590-019-0416-z.pdf
... uniquely human immune ...with human cells or tissues — i.e., ‘humanized’ mice or ‘human immune system’ (HIS) mice ...with human cells and tissues serve as a preclinical bridge ...
5
Recovery of Pathogenic Measles Virus from Cloned cDNA
... B95a cells (34, 35), as a tag (Fig. 1). Pathogenic MeV isolated in B95a cells will grow only in lymphoid cells such as human peripheral blood mononuclear cells ...
5
A Preponderance of CCR5+CXCR4+ Mononuclear Cells Enhances Gastrointestinal Mucosal Susceptibility to Human Immunodeficiency Virus Type 1 Infection
... and PBMCs, viral differences between the blood and mucosal compartments might also determine infec- ...and peripheral blood samples of HIV-1-seropositive pa- ...to peripheral ...
10
Establishment of B-Cell Lymphoma Cell Lines Persistently Infected with Hepatitis C Virus In Vivo and In Vitro: the Apoptotic Effects of Virus Infection
... primary human hepatocytes, peripheral blood mononuclear cells (PBMCs), and an established B-cell line (Raji cells) in ...SB cells belongs to genotype ...SB ...
13
Activation of Peripheral Blood Mononuclear Cells by Dengue Virus Infection Depotentiates Balapiravir
... in PBMCs that are preinfected with DENV, two other factors may contribute to the lack of efficacy in dengue ...primary human hepatocytes at 2 M ...
8
In vitro immunologic and virologic effects of interleukin 15 on peripheral blood mononuclear cells from normal donors and human immunodeficiency virus type 1-infected patients.
... in PBMCs from some HIV-seropositive and HIV-negative do- nors in a dose-dependent ...these PBMCs was ...that human monocytes can produce IL-15 protein and that this endogenous IL-15 contributes to ...
6
Enhanced frequency and potential mechanism of B regulatory cells in patients with lung cancer
... Peripheral blood mononuclear cells (PBMC) were iso- lated as previously described ...whole blood samples were overlaid onto Ficoll separation media (Tianjin Haoyang Biological ...
11
Human breast cancer cells educate macrophages toward the M2 activation status
... HER-2: human epidermal growth factor receptor 2; HGFR: hepatocyte growth factor receptor; IFN- γ : interferon γ ; kDa: kiloDaltons; LIF: leukemia inhibitory factor; LPS: lipopolysaccharide; M-CSF: monocyte colony ...
14
MicroRNA 155 as a proinflammatory regulator via SHIP 1 down regulation in acute gouty arthritis
... in peripheral blood mononuclear cells (PBMCs) from healthy controls (HCs), PBMCs and synovial fluid mononuclear cells (SFMCs) of gouty arthritis ...(B) PBMCs ...
9
Trastuzumab mediates antibody dependent cell mediated cytotoxicity and phagocytosis to the same extent in both adjuvant and metastatic HER2/neu breast cancer patients
... NK cells, which supports the notion that CD32 is primarily expressed on monocytes and is enriched dur- ing phagocytosis ...encoding human Fc receptors has been reported that leads to inhibitory CD32 ...
11
The effects of BAFF on T lymphocytes in chronic obstructive pulmonary disease
... heparinized peripheral ven- ous blood of COPD patients using density centrifuga- ...fresh peripheral blood was centrifugated at 1500 rpm for 10 min, then the supernatant containing leukocytes ...
10
Hepatocellular carcinoma associated antigen 59 of Haemonchus contortus modulates the functions of PBMCs and the differentiation and maturation of monocyte derived dendritic cells of goats in vitro
... h. Cells were then washed and permitted to settle onto poly-l-lysine treated glass slides for 30 min, after which they were fixed with 4% paraformaldehyde in PBS for 30 min at room tem- perature ...Next, ...
14
Envelope Glycoprotein Binding to the Integrin α4β7 Is Not a General Property of Most HIV-1 Strains
... exposure, human immunodeficiency virus type 1 (HIV-1) replicates at low levels due to the insufficient num- bers of CD4 ⫹ T cell targets to ...T cells are present, a high level of replication takes place, ...
11
Oxidative stress and Nrf2 expression in peripheral blood mononuclear cells derived from COPD patients: an observational longitudinal study
... Obstructive Lung Disease (GOLD) 1 and GOLD 2 stage COPD patients and of Nrf2 mRNA as previously re- ported [14, 16]. Adopting a level of significance of p = 0.05, a power of 80% and a minimum correlation of 0.40, we ...
12
Evaluation of Regulatory T Cells in Patients with Acute and Chronic Brucellosis
... venous blood samples were collected from the patients and controls and stored in tubes containing ethylenediamine tetraacetic acid ...the blood samples were transferred into round-bottomed centrifuge tubes ...
6