Human peritoneal mesothelial cells
Glucose-Based Peritoneal Dialysis Fluids Downregulate Toll-Like Receptors and Trigger Hyporesponsiveness to Pathogen-Associated Molecular Patterns in Human Peritoneal Mesothelial Cells
... 31). Peritoneal mesothelial cells (PMCs) have been shown to constitutively express TLR1, -2, -3, -4, -5, and ...LPS-induced peritoneal inflammation, in an NF- B-dependent manner ...local ...
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Original Article Histone acetyltransferase inhibitor C646 reverses epithelial to mesenchymal transition of human peritoneal mesothelial cells via blocking TGF-β1/Smad3 signaling pathway in vitro
... after cells were cross-liked with 1% formaldehyde for 10 min, cells were neutralized in glycine and collected by ...ice. Cells were sonicated to shear DNA to lengths between 200-1000 ...
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Original Article Dioscin ameliorates peritoneal fibrosis by inhibiting epithelial-to-mesenchymal transition of human peritoneal mesothelial cells via the TLR4/ MyD88/NF-κB signaling pathway
... HMrSV5 cells were seeded in six-well plates for 24 ...μg/ml), cells were fixed in 4% formaldehyde in PBS for 5 min, per- meabilized with ...fixed cells were incu- bated with anti-α-SMA antibody ...
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Transcriptome analysis of signaling pathways of human peritoneal mesothelial cells in response to different osmotic agents in a peritoneal dialysis solution
... of human peritoneal meso- thelial cells (HPMCs) using a whole-transcriptome ana- lysis after repeated exposure to HPG-based PD solution as compared to glucose-based conventional ...
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Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, in vitro and in vivo
... Human peritoneal mesothelial cells were cultured to sub- confluence in a 50-cm 2 dish with DMEM containing 10 % FCS. The media were then changed to (1) SF-CM from gastric cancer cell MKN-45, ...
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Production of IL1-beta by ovarian cancer cells induces mesothelial cell beta1-integrin expression facilitating peritoneal dissemination
... the peritoneal cavity ...tumor cells or the mesothe- lium if we can identify those targets that are biologically essential to peritoneal ...and human epidermal growth factor receptors [20,21] ...
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Asbestos and erionite prime and activate the NLRP3 inflammasome that stimulates autocrine cytokine release in human mesothelial cells
... in human mesothelial cells, responses that trigger transcription and production of cytokines critical to the initiation of pleural injury and infection, MM and pleural ...primary human pleural ...
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Mesothelial cells promote early ovarian cancer metastasis through fibronectin secretion
... these cells in the molecular events underlying the early dissemination of abdominally metastasizing tumors remains poorly ...The peritoneal cavity and the omentum are lined by the meso- thelium, a monolayer ...
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Metalloproteinases and tissue inhibitor of metalloproteinases in mesothelial cells Cellular differentiation influences expression
... Mesothelial cells play a critical role in the remodeling process that follows serosal ...Although mesothelial cells are known to synthesize a variety of extracellular matrix components ...
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Functional molecules in mesothelial to mesenchymal transition revealed by transcriptome analyses
... the TGF-β1-induced depletion of BMP4 might itself modulate MMT. Indeed, recombinant BMP4 partially prevented TGF-β1-induced MMT, as indicated by reten- tion of membranous ZO1 localization, lack of αSMA induction and ...
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Epithelial to Mesenchymal Transition of Mesothelial Cells in Tuberculous Pleurisy
... the cells were isolated by centrifu- gation and cultured until grown to confluence for 7-10 ...of cells in confluent cultures were compared and remained stable during the two cell ...All cells were ...
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Karyotype alteration generates the neoplastic phenotypes of SV40-infected human and rodent cells
... infected human, rat, and mouse cells from infection to the evolution of rare immortal neoplastic ...infected cells, which is incompatible with direct viral transformation, (3) neo- plastic clones ...
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B2 but Not B1 Cells Can Contribute to CD4+ T-Cell-Mediated Clearance of Rotavirus in SCID Mice
... immunocompetent cells allows one to ex- amine the potential roles of these cells in the ablation of viral shedding as well as in the protection against ...
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Mechanisms of oxidative stress and alterations in gene expression by Libby six-mix in human mesothelial cells
... from cells or from reduction-oxidation reac- tions occurring on the surface of high iron-containing fibers (reviewed in ...on cells of the lung and pleura as do amphibole types of asbestos such as ...the ...
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Asbestos induces apoptosis of human and rabbit pleural mesothelial cells via reactive oxygen species
... Apoptotic cells are characterized by a highly condensed nucleus that stains vividly with DNA dyes; in early apoptosis, acridine orange en- ters the cell but propidium iodide is excluded and the nucleus is stained ...
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Persistent infection of normal mice with human immunodeficiency virus.
... Cocultivation of PBMC, peritoneal cells, and spleen cells with human uninfected U937 or CEM a T lymphoma cell line cells resulted in HIV infection of the target cells, as determined by P[r] ...
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Antibody to a Lytic Cycle Viral Protein Decreases Gammaherpesvirus Latency in B-Cell-Deficient Mice
... decreased frequency of genome-bearing peritoneal cells (P ⫽ 0.0001; Fig. 4B, left panel) compared to that for the mice that received preimmune serum, M2-specific antiserum, or v-cyclin- specific antiserum. ...
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CRIg-expressing peritoneal macrophages are associated with disease severity in patients with cirrhosis and ascites
... Patients and ascites fluid collection. Ascitic fluid (2–4 liters) was collected from patients with decompensated cir- rhosis undergoing therapeutic paracentesis at the Princess Alexandra Hospital. Standard biochemical ...
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Angiotensin II Increases Host Resistance to Peritonitis
... tion of the effect of AII on abscess formation shows that the in vitro observation could be translated into a therapeutic benefit in this model. As AII acted on the host as an immunomodu- lator rather than on the ...
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Binding of Lys plasminogen to monocytes/macrophages
... macrophages from patients with indwelling catheters and tissue macrophages in chronic inflammatory lesions were shown to express immunologically identified Lys-plasminogen on their surfaces. Therefore binding and surface ...
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