human β-defensin-1
Single Nucleotide Polymorphisms (SNPs) in Human β Defensin 1: High Throughput SNP Assays and Association with Candida Carriage in Type I Diabetics and Nondiabetic Controls
... Nevertheless, not all diabetic patients experience fungal in- fections or exhibit signs and symptoms associated with fungal infection (5). This phenomenon is also evident in the nondia- betic population, in which some ...
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Antimicrobial peptide β-defensin-1 expression is upregulated in Alzheimer’s brain
... Mammalian β-defensins are an evolutionarily conserved group of cationic, non-glycosylated antimicrobial and immunomodulatory peptides that are expressed in all mammals, including humans ...pressed human ...
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Synergistic effect of interleukin 1 alpha on nontypeable Haemophilus influenzae induced up regulation of human beta defensin 2 in middle ear epithelial cells
... to human upper respiratory epithelial cells ...as β -defensin 2 ...and β-defensin subfamilies on the basis of the position and bonding of six conserved cysteine ...The β ...
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Respiratory epithelial cells require Toll-like receptor 4 for induction of Human β-defensin 2 by Lipopolysaccharide
... USA) 1:100 dilution) or isotype control (1:1000 dilution of 1 mg/ml stock of iso- type goat IgG) and incubated at 4°C in the dark for 30 ...with 1:10 dilution of anti-goat IgG fluorescein ...
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THE RELATIONSHIP BETWEEN INTESTINAL PROTECTIVE MARKERS AND SERUM ENDOTHELIN 1 CONCENTRATIONS IN LOW BIRTH WEIGHT INFANTS WITH HYPOXIC ISCHEMIC ENCEPHALOPATHY
... The results of previous investigations have shown that different inflammatory factors may contribute to the pathogenesis of intestinal barrier injury, and most studies have focused on the pathological roles of cytokines ...
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Isolation of human β-defensin-4 in lung tissue and its increase in lower respiratory tract infection
... in 1 µ g/ml proteinase K (DakoCytomation) for 30 min at 37°C and treated with 6% hydrogen peroxidase for 60 min to inactivate endogenous ...with 1 µg ...
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Isoleucine, an Essential Amino Acid, Induces the Expression of Human β Defensin 2 through the Activation of the G Protein Coupled Receptor ERK Pathway in the Intestinal Epithelia
... After the incubation of Caco2 cells with inducers such as Ile for various times, the cells were treated with Cell Lysis Buffer (Cell Signaling) with a Protease Inhibitor Cocktail Kit (PIERCE, Rockford, USA) as described ...
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Fecal human β-defensin-2 (hBD-2) levels and gut microbiota patterns in preterm neonates with different feeding patterns
... Measurement of hBD-2. Fecal hBD-2 was mea- sured using a specific enzyme-linked immune assay (ELISA) according to the manufacturer’s instruc- tions (β–defensin 2; Immunodiagnostic AG, Ben- sheim, Germany; ...
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Keratinocyte Expression of Human β Defensin 2 following Bacterial Infection: Role in Cutaneous Host Defense
...  defensin 2 (hD-2) is a cationic antimicrobial peptide (CAP) found in the skin and is thought to play impor- tant roles in cutaneous host immune defense and numerous disease ...interleukin 1 ...
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Human β defensin 2 plays a regulatory role in innate antiviral immunity and is capable of potentiating the induction of antigen specific immunity
... addition, β-defensins have been reported to function as chemoattractants for vari- ous antigen-presenting cells (APCs), including immature DCs and macrophages, through chemokine receptors CCR6 and CCR2 [9, 34, ...
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Human β-defensin-2 production from S. cerevisiae using the repressible MET17 promoter
... Heterologous expression systems have the potential to provide hBD2 for both clinical research and therapeu- tic applications. However, the issues of host cell toxicity, proteolytic degradation, folding and low yields ...
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Clinical application of human β defensin and CD14 gene polymorphism in evaluating the status of chronic inflammation
... Periodontitis is a chronic infectious disease[1] involving the degradation and destruction of periodontal support- ing tissue of the teeth[2]. This disease is highly prevalent and can affect up to 90% of the ...
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PPARγ as a sensor of lipase activity and a target for the lipase inhibitor orlistat
... the human intestinal epithelial cell line Caco-2, at concentrations of 1 - 100 μ M, leads to induction of genes regulated by PPAR γ ...of β -defensin 1 (hDB1) & Adipose ...
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Antimicrobial activity of innate immune molecules against Streptococcus pneumoniae, Moraxella catarrhalis and nontypeable Haemophilus influenzae
... to β defensin- 2 or ...µg/ml β defensin-2 for 30 minute, and 1 mg/ml of human milk lysozyme for 30 minute, ...than 1 mg/ml, had no observable effect on the ultrastructure ...
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Host Defense Peptides in Wound Healing
... induces human β-defensin 2 (88,89). Further- more, human Toll-like receptor 2 medi- ates induction of the antimicrobial pep- tide hBD-2 in response to bacterial lipoprotein ...
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The potential for immunoglobulins and host defense peptides (HDPs) to reduce the use of antibiotics in animal production
... of human neutrophil defensin-1 ...approx. 1 log unit in lungs, liver and spleen, whereas combined therapy with anti-TB drugs and HNP-1 augmented reduction of bacterial loads by eight- ...
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Structural and functional characterisation of a novel bovine β defensin gene cluster
... B-actin Antimicrobial peptides Activator protein 1 Antigen presenting cell Spermadhesins aqnl Adenosine triphosphate transferase Bovine beta defensin Bovine endometrium cells sperm assoc[r] ...
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A study on β-defensin-2 and histatin-5 as a diagnostic marker of early childhood caries progression
... The study was conducted between 2010 and 2013 and included 82 pediatric patients from the Department of Pediatric Dentistry, Institute of Dentistry, Jagiellonian University, Medical College in Krakow, Lesser Poland ...
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Low serum vitamin D levels in type 2 diabetes patients are associated with decreased mycobacterial activity
... The clinical characteristics of the study groups are pre- sented in Table 1. The T2D patients was significantly older and had higher levels of fasting glucose, glycated haemoglobin A (HbA1c), cholesterol, LDL and ...
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Canine β defensin 1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs
... For L. infantum detection, the primers LEISH-1 (5'-AAC TTT TCT GGT CCT CCG GGT AG-3'), LEISH-2 (5'- ACC CCC AGT TTC CCG CC-3') and the TaqMan® probe FAM-5'-AAA AAT GGG TGC AGA AAT-3'-non- fluorescent quencher-MGB ...
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