hydrolytic activity
In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis
... In previous work with Ustilago maydis, using EBI fungicides and mutations in the genes encoding enzymes targeted by them, we have presented evidence that alteration of the normal sterol profile produces changes in the ...
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Effects of efrapeptin and destruxin, metabolites of entomogenous fungi, on the hydrolytic activity of vacuolar type ATPase identified on the brush border membrane vesicles of Galleria mellonella midgut and on plant membrane bound hydrolytic enzymes
... have identi¢ed a similar enzyme in the BBMV from G. mellonella. Firstly, marker enzyme analysis indi- cates that we have successfully puri¢ed the BBMV fraction from the tissue homogenate. The activities of alkaline ...
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Hydrolytic activity of amylase produced in solid - state fermentation by a local isolate of Aspergillus niger
... the activity of amylase showed that the enzyme activity increased progressively with an increase in temperature from 30 o C ...amylase activity may be attributed to the disruption of the secondary, ...
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MICROBIAL CONTAMINANTS OF DATE PALM (PHOENIX DACTYLIFERA L.) IN IRAQI TISSUE CULTURE LABORATORIES
... of hydrolytic activity of phenol oxidase and cellulase in vitro for several date palm tissue culture fungal contaminants including Aspergillius niger and Alternaria ...oxidase activity, Aspergillius ...
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Muutused mulla mikroobide hüdrolüütilises aktiivsuses ja orgaanilise süsiniku sisalduses punase ristiku allakülviga odra kasvatamisel
... The experiments were carried out in the Eerika test site of Rõhu experimental station of Institute of agricultural and environmental sciences, Estonian University of Life Sciences during 2012–2016. There were 5 crops in ...
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Influence of sulfite on ATPase activity of coupling factor CF(1) isolated from spinach chloroplasts
... the hydrolytic activity of CF 1 is blocked in the dark but it can grow under reduction of the di- sulfide bond of the γ-subunit due to light energiza- tion of chloroplasts ...ATPase activity of CF 1 ...
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Synthesis of Benzyl Acetate Catalyzed by Lipase Immobilized in Nontoxic Chitosan-Polyphosphate Beads Ana D.Q. Melo 1* , Francisco F.M. Silva 2, José C.S. dos Santos3 , Roberto Fernández-Lafuente 4,* Telma L.G. Lemos 5and Francisco A. Dias Filho6
... the hydrolytic activity of the enzyme in the different solvents was different, the immobilized enzyme was active in all of ...higher hydrolytic activity of the ...
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Extraction, Purification and Characterization of Protease from Aspergillus Niger Isolated from Yam Peels
... The important feature of proteases is their ability to discriminate among competing substrates and utility of these enzymes often depends on their substrate specificity (Shankar et al., 2011). The purified protease was ...
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Intestinal Assimilation of a Tetrapeptide in the Rat: OBLIGATE FUNCTION OF BRUSH BORDER AMINOPEPTIDASE
... Intestinal transport of glucose, amino acids, and peptides 16 occurs uphill against a concentration gradient by energy-dependent processes but hydrolytic activity of brush border amino-o[r] ...
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The effect of short and long term fasting on digestive and metabolic flexibility in the Andean toad, Bufo spinulosus
... the hydrolytic activities of digestive enzymes, the histology of the small intestine, the gross morphology of digestive and other internal organs and, finally, the whole organism metabolic ...the hydrolytic ...
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HALOBACTERIAL A ATP SYNTHASE IN RELATION TO V ATPase
... The ATP hydrolytic activity of solubilized membrane of various halobacterial strains Activity nmol Pi min"1 mg ' protein Strain Halobacterium halobium RimR Halobacterium halobium DSM670 [r] ...
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PARTIAL PURIFICATION AND CHARACTERISATION OF COLD-ACTIVE METALLOPROTEASE BY BACILLUS SP. AP1 FROM APHARWAT PEAK, KASHMIR
... on activity and stability of purified protease: Hydrolytic activity of purified enzyme was measured at varying temp- eratures to determine the maximum activity which was found to be 20°C, ...
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Hydrolytic and Transesterification Activities of Thermostable Lipase ITB1.1
... Lipase activity was measured by spectrophotometer at = 405 nm, based on the release of p-Nitrophenol (pNP) from pNPP ...Lipase activity expressed in units, where 1 unit is defined as 1 µmol PNP product ...
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Inhibition ofTrichoderma cellulase activity by a stilbene glucoside fromPicea glehnii bark
... First, the inhibitory effect of the stilbene glucoside isorhapontin (I) on the hydrolytic activity of CBH I for bacterial microcrystalline cellulose (BMCC) and cellohepta[r] ...
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Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae
... We targeted the β-glucosidase from A. niger (BGL1) using directed evolution to adapt it towards heterolo- gous expression S. cerevisiae. We built a fusion protein by replacing the native BGL1 signal sequence with the S. ...
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Optimizing the preparation conditions and characterization of a stable and recyclable cross-linked enzyme aggregate (CLEA)-protease
... It has been revealed that one of the major and primary reasons of enzyme immobilization is the anticipated increase in its stability towards various deactivating conditions; it takes place because of the limited con- ...
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Phosphodiesterase type 4 expression and anti-proliferative effects in human pulmonary artery smooth muscle cells
... lial-derived factors and growth factors [23,24]. Proteolytic enzymes are also thought to be involved, including elastase and matrix metalloproteinases (MMP) such as the gelatinases MMP-2 and MMP-9, which degrade collagen ...
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Molecular and Biochemical Characterization of a Novel β-N-Acetyl-D-Hexosaminidase with Broad Substrate-Spectrum from the Aisan Corn Borer, Ostrinia Furnacalis
... pNP-β-GlcNAc activity as OfHex2) and lysozyme from chicken egg white were used as positive con- ...lysozyme activity, but OfHex2 did not show any activity toward peptidoglycan substrate (data not ...
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Bioprospecting for culturable psychrotrophic bacteria with extracellular hydrolytic enzymes activity from arctic lake sediments
... The ability of cold-adapted microorganisms to produce cold-active enzymes with potential biotechnological applications has recently attracted the attention of scientific community in terms of bioprospecting. This ...
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Characterization of a copper responsive promoter and its mediated overexpression of the xylanase regulator 1 results in an induction-independent production of cellulases in Trichoderma reesei
... Cellulase production in T. reesei is a highly coordi- nated process regulated by a suite of transcription fac- tors with XYR1 being considered the main activator of cellulase/hemicellulase gene expression [4,5]. Regula- ...
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