Hydrophilic interaction chromatography
The rise of hydrophilic interaction chromatography in untargeted clinical metabolomics
... Hydrophilic interaction chromatography (HILIC) was introduced more than two decades ago and has garnered much ...a hydrophilic stationary phase used in combination with an aqueous organic ...
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Glycosylation Site Analysis of Human Platelets by Electrostatic Repulsion Hydrophilic Interaction Chromatography
... repulsion hydrophilic interaction chromatography, clearly enabled the accumulation of glycopeptides with an effi- ciency demonstrated also for previously used approaches like ...
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Evaluating the Adsorbed Water Layer on Polar Stationary Phases for Hydrophilic Interaction Chromatography (HILIC)
... Abstract: The water-rich liquid layer immobilized on the surface of the polar stationary phases is critical to the retention of polar compounds in hydrophilic interaction chromatography (HILIC). ...
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A method for the analysis of sugars in biological systems using reductive amination in combination with hydrophilic interaction chromatography and high resolution mass spectrometry
... 3. Results 3.1 Underivatised Sugars Figure 1 shows extracted ion chromatograms obtained for underivatised glucose and galactose on a ZICpHILIC column with our standard metabolomics screening conditions [21] which uses ...
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A study of column equilibration time in hydrophilic interaction chromatography
... in hydrophilic interaction liquid chromatography (HILIC) was compared using the mobile phase disturbance method, column efficiency measurements and retention time ...
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A study of the analysis of acidic solutes by hydrophilic interaction chromatography
... neutral, basic and acidic solutes was much more similar to one another on the amide column, in accord with the lack of ionic repulsion effects for basic and attractive effects for acidic solutes that had occurred on the ...
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Performance of charged aerosol detection with hydrophilic interaction chromatography
... However, hydrophilic solutes gave good response at higher evaporation temperatures (up to 80 ◦ C) as they may be retained longer in the aqueous portion of the aerosol ...
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Comparison of the determination of folate vitamers with hydrophilic interaction chromatography and reversed phase chromatography
... purpose hydrophilic liquid interaction chromatography, short HILIC, could be the preferred choice of ...liquid chromatography with the benefit of being able to retain polar ...liquid ...
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Study of retention and peak shape in hydrophilic interaction chromatography over a wide pH range
... Surprising and useful differences in selectivity were obtained on a given column by the use of stronger acid additives such as TFA or HFBA compared with ammonium salt buffers. Pronounced retention of strongly acidic ...
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Some factors that can lead to poor peak shape in hydrophilic interaction chromatography, and possibilities for their remediation
... 3.4 Problems with metals and possible remediation Problems resulting from solute-metal interactions have been studied in RP separations [20-23], particularly with regard to the analysis of compounds of biological ...
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Accurate and efficient amino acid analysis for protein quantification using hydrophilic interaction chromatography coupled tandem mass spectrometry
... liquid chromatography (UHPLC/UPLC or HPLC)-, gas chromatography (GC) or capillary electro- phoresis (CE) prior to detection by absorbance, fluores- cence, or mass spectrometry ...
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Hydrophilic-Interaction Chromatography (HILIC) for LC-MS/MS Analysis of Monoamine Neurotransmitters using XBridge BEH Amide XP Columns
... RESULTS AND DISCUSSION: Optimization of Mobile Phase Composition Figure 1A shows the chromatography of monoamine neurotransmitters analyzed on the XBridge BEH Amide XP column. In order to achieve acceptable peak ...
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Comparison of the kinetic performance and retentivity of sub 2μm core shell, hybrid and conventional bare silica phases in hydrophilic interaction chromatography
... results show an appreciable difference between the effective diffusion behaviours of cytosine and nortriptyline. On average, D eff /D m for nortriptyline was around 40% larger than for cytosine. In particular, the ratio ...
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HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY (HILIC) AND ITS APPLICATION IN PHARMACEUTICAL ANALYSIS
... Alpert coined the term,‟Hydrophilic interaction chromatography” in 1990. This mode of chromatography dates back to earliest days of liquid chromatography. It happened when two ...
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Hydrophilic interaction liquid chromatography: An update
... 2 Chromatogram of a mixture of acidic basic and neutral test compounds on a BEH amide column using 95% ACN containing 5 mM ammonium formate buffers w/w. pH 3.0, 4.4, ammonium acetate [r] ...
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Understanding and manipulating the separation in hydrophilic interaction liquid chromatography
... plotting hydrophilic selectivity (measured from alpha cytosine/uracil) against (cat) ionic interaction (measured from alpha benzyltrimethylammonium ...both hydrophilic and structurally similar ...
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Quantitative structure retention relationships for rapid method development in hydrophilic interaction liquid chromatography of pharmaceutical compounds
... 152 The model term ranking chart, presented in Figure 4.6 allows determination of the most significant factors in determining retention of analytes. The data in Figure 4.6 indicate that the linear and the quadratic terms ...
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Effect of mobile phase additives on solute retention at low aqueous pH in hydrophilic interaction liquid chromatography
... Title: Effect of mobile phase additives on solute retention at low aqueous pH in hydrophilic interaction liquid chromatography.. Article Type: Full length article.[r] ...
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Practical observations on the performance of bare silica in hydrophilic interaction compared with C18 reversed phase liquid chromatography
... a hydrophilic-neutral solute (5-(hydroxymethyl)uridine), hydrophilic-weak base (cytosine), hydrophobic-strong base (nor- triptyline) and a hydrophobic-neutral ...
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Title: Determination of thiopurine S-methyltransferase activity by hydrophilic interaction liquid chromatography hyphenated with mass spectrometry
... Performance parameters of the optimized RP-HPLC-UV, RP-HPLC-MS, and HILIC- HPLC-MS methods were evaluated for the 6-MMP standard present in the model (spiked) RBC lysate matrix, and the[r] ...
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