In-frame gene deletion
Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach
... alcohologenesis-specific gene(s) responsible for alcohol formation was predicted because (i) there was high NADH-dependent butanol dehydrogenase activity in alcohologenesis versus high NADPH-dependent butanol ...
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Gene Deletion by Fluorescence Reported Allelic Exchange Mutagenesis in Chlamydia trachomatis
... trpA deletion by ...for deletion as proof of ...reading frame was removed and replaced with an ...mCherry gene was FIG 2 pSU6 maintenance by ...
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Deletion of the VP16 open reading frame of herpes simplex virus type 1.
... Deletion mutant virions propagated in this cell line contained wild-type, cell-derived VP16 protein that was recruited during virion assembly and was functional for immediate-early gene [r] ...
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Mutations within the 5' half of the avian retrovirus MC29 v-myc gene alter or abolish transformation of chicken embryo fibroblasts and macrophages.
... A biological and biochemical analysis, carried out on a series of in-frame insertion and deletion mutations within the gag-myc gene of MC29, revealed several mutations within the 5' port[r] ...
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Deletion of gpUL132, a Structural Component of Human Cytomegalovirus, Results in Impaired Virus Replication in Fibroblasts
... the gene product of reading frame ...the gene is transcribed with a true late kinetics from the laboratory- adapted strain AD169 and the low-passage isolate ...UL132 gene deleted were ...
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A Chinese patient with 11β-hydroxylase deficiency due to novel compound heterozygous mutation in CYP11B1 gene: a case report
... CYP11B1 gene disclosed two novel mutations (Fig. 3): a heterozygous in-frame insertion de- letion mutation ...the deletion mutation inherited from the mother, the mu- tation ...
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Attenuation of Marek's Disease Virus by Deletion of Open Reading Frame RLORF4 but Not RLORF5a
... that deletion of RLORF5a in the CVI988 strain did not affect in vitro and in vivo replication and the establishment of latency (31); however, its involvement in oncogenesis could not be studied since CVI988 is ...
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Sequence of hepatitis B virus DNA incorporated into the genome of a human hepatoma cell line.
... By sequencing the region between the left junction 13vhl and the deletion 13vvl, we determined that the core antigen gene is shifted out of frame at position 1960 in the HBV sequence by [r] ...
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Replicase Complex Genes of Semliki Forest Virus Confer Lethal Neurovirulence
... nsp3 gene of rA774 displayed an opal termination codon and an in-frame 21-nucleotide deletion close to the nsp4 ...nsp3 gene with that of SFV4 reconstituted the virulent phenotype, whereas an ...
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Effects of an In-Frame Deletion of the 6k Gene Locus from the Genome of Ross River Virus
... 6k gene region, thereby ensuring that the translation of proteins encoded by sequences on either side of the 6k gene region remains ...partial gene deletions or insertions, as were done previously ...
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Molecular Genetics of Autosomal Recessive Spinal Muscular Atrophy
... (1995) A frame-shift deletion in the survival motor neuron gene in Spanish spinal mus- cular atrophy patients. Roy N, Mahadevan MS, McLean M, et al[r] ...
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Deletion mutants of herpesvirus saimiri define an open reading frame necessary for transformation.
... Previous studies have identified a region of the herpesvirus saimiri genome required for immortalization of marmoset lymphocytes in vitro and for oncogenicity in vivo; this region is not[r] ...
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Human genetic susceptibility to mother to child transmission of HIV : a study of mother-infant pairs in Malawi
... DC-SIGN gene regulates DC-SIGN, which when in cis with CD4 and a coreceptor, facilitates more efficient entry of HIV and SIV, an effect more notable when the CD4 or coreceptors are limited 89 ...the gene ...
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Engineering Bacillus licheniformis for the production of meso-2,3-butanediol
... block D-2,3-BD formation (Fig. 1). The engineered B. licheniformis WX-02Δgdh strain significantly increased meso-2,3-BD production and completely eliminated the production of the competing product D-2,3-BD. Second, the ...
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Choroid plexus transport: gene deletion studies
... Thus, deletion of the Na/bicarbonate trans- porter, Nbcn2, alters the CP location of the Na + /H + exchanger, Nhe1, a transporter that is also involved in pH regulation ...the gene of interest can be ...
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Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
... To achieve simultaneous multiple knock-outs in O. polymorpha, three genes OpURA3, OpHIS3 and OpLEU2 were selected as the target loci. The UHA and DHA (~ 1 kb) fragments of each target gene were joined by Splicing ...
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Tc-knirps plays different roles in the specification of antennal and mandibular parasegment boundaries and is regulated by a pair-rule gene in the beetle Tribolium castaneum
... segmentation gene cascade, involving mater- nal, gap, pair-rule and segment polarity genes [3,8], while the patterning of the procephalic segments follows a different paradigm ...polarity gene expression ...
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The r144 Major Histocompatibility Complex Class I-Like Gene of Rat Cytomegalovirus Is Dispensable for both Acute and Long-Term Infection in the Immunocompromised Host
... r144 gene encodes an MHC class I ...r144 gene, which putatively encodes an MHC class I ...putative gene products of r144, m144, and UL18 as gpr144, gpm144, and gpUL18, ...
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Genetic testing for hearing loss in the United States should include deletion/duplication analysis for the deafness/infertility locus at 15q15.3
... Background: Hearing loss is the most common birth defect and the most prevalent sensorineural disorder in developed countries. More than 50% of prelingual deafness is genetic, most often autosomal recessive and ...
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A gene coding for the uric acid-xanthine permease of Aspergillus nidulans: inactivational cloning, characterization, and sequence of a cis-acting mutation.
... In a strain with a large deletion in the gene coding for acetamidase (amdS), a plasmid carrying this gene integrates into and inactivates uapA, the putative structu[r] ...
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