Induced hepatocyte-like cells
The therapeutic potential of induced hepatocyte-like cells generated by direct reprogramming on hepatic fibrosis
... In this study, we used iHEPs by adopting two different direct reprogramming techniques. One is generated by retroviral introduction of specific transcription factors c-Myc and Klf-4 and the other is by using small mole- ...
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Analysis of differentially expressed genes among human hair follicle–derived iPSCs, induced hepatocyte-like cells, and primary hepatocytes
... feeder cells suitable for iPSC ...feeder cells in HES medium (80% DMEM/F12 supplemented with 20% KSR, 1% nonessential amino acids, 1 mM L -glutamine, 4 ng/ml human bFGF, and ...
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Efficient Differentiation of Human Induced Pluripotent Stem Cell (hiPSC) Derived Hepatocyte-Like Cells on hMSCs Feeder.
... Subsequently, cells were treated with oncostatin M plus dexamethasone for 9 days to generate hepatic ...as, cells differentiated with a hepatocyte-like morphology was also ...
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Stem cell-derived models to improve mechanistic understanding and prediction of human drug-induced liver injury
... human cells for screening for adverse effects of drugs in ...of induced pluripotent stem cells means that it may ultimately be possible to develop personalised toxicology to determine ...
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Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D
... human induced pluripotent stem cells (hiPSCs), their response to a valve-based printing process as well as their post-printing differentiation into hepatocyte-like cells ...stem ...
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Hepatocyte Growth Factor-induced Differentiation of BMSCs toward Hepatocyte-like Cells via the NF-κB and P38MAPK Signaling Pathway
... stem cells(BMSCs) have great potential ability of multi-directional differentiation and reproductive ...be induced into hepatocyte-like ...was induced by 10% fetal bovine serum with ...
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Hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study
... times, cells were then permeabilized by ...permeabilization, cells were blocked with 1% (v/v) bovine serum albumin (BSA) (Invitrogen; Carlsbad, CA) in PBST (PBSTB) for 30 min at 25 ...PBSTB, cells ...
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Original Article Skeletal muscle-derived stem cells differentiate into hepatocyte-like cells and aid in liver regeneration
... into hepatocyte-like cells using a co-culture system with two differ- ent liver cell ...Hepa1-6 cells. Day 1 of MDSCs co-cultured with AML12 or Hepa1-6 cells showed no expression of ...
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Phenotype instability of hepatocyte-like cells produced by direct reprogramming of mesenchymal stromal cells
... impair hepatocyte maturation in ...successfully induced the expression of Foxa2 ...These cells (d-iHEPs) were purified within 6 passages and were found to ex- press CK18 and albumin, as shown by ...
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The Effect of Bay 11-7082 Inhibits Rat BMSCs to Hepatocyte-Like Cells via NF-κB Signaling in Vitro
... To ICG assay, the differentiation was not complete in all BMSCs, accordingly AAT (a glycoprotein synthesized chiefly in hepatocytes) expression has a good linear relationship with the degree of differentiation. As shown ...
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Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte like cells
... promoted hepatocyte differ- entiation. FGF-4 alone induced hepa- tocyte ...when cells were also treated with ...to cells with a hepatocyte phenotype was seen only when cells were ...
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A robust model of natural hepatitis C infection using hepatocyte like cells derived from human induced pluripotent stem cells as a long term host
... carcinoma cells (Huh7 and their deriva- tives) ...derived cells [12, ...human hepatocyte. The cancer cells actively entered cellular division while the primary hepatocytes were mostly in ...
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Rapid generation of functional hepatocyte-like cells from human adipose-derived stem cells
... epithelial cells, was upregu- lated as demonstrated by RT-PCR and Western blot analysis ...epithelial cells from mesenchymal ...the cells was further sub- stantiated by the appearance of functional ...
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Effect of Mouse Liver Extract on in Vitro Differentiation of Amniotic Membrane Stem Cells into Hepatocyte-Like Cells
... into hepatocyte-like cells occurred within the 16- day ...these cells, which indicated the successful differentiation of these cells into hepatocyte-like ...stem ...
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Alcohol Increases Liver Progenitor Populations and Induces Disease Phenotypes in Human IPSC-Derived Mature Stage Hepatic Cells
... progenitor cells remains largely unknown. Here, we used human induced pluripotent stem cells (iPSCs) as a model to examine the effects of alcohol, on multi-stage hepatic cells including ...
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Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening
... ADR is a major issue in the pharmaceutical industry as it is responsible for attrition of drugs during development and withdrawal of drugs post-licensing [35]. Among ADRs, drug-induced liver injury (DILI) is the ...
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Generation of functional human hepatocytes in vitro: current status and future prospects
... of cells (Table ...these cells for transplantation therapy. Liu et al. generated hu- man induced pluripotent stem cell (iPSC) lines from dif- ferent sources and intravenously transplanted definitive ...
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Novel approaches using human induced pluripotent stem cells and microRNAs in the development of relevant human hepatocyte models for drug-induced liver injury
... optimal hepatocyte function which limits the utility of the hPH model to acute toxicity studies only, and compromises their use in mechanistic studies of DILI which often occurs following prolonged exposure to ...
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Development of Simple Protocol for Generation of Functionally Active Hepatocyte-like Cells from Human Adipose Tissue-derived Stem Cells
... generating hepatocyte-like ...produced hepatocyte-like cells exhibit poor functionality, mainly due to the lack of appropriate induction ...in hepatocyte differentiation ...with ...
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Recapitulation of methotrexate hepatotoxicity with induced pluripotent stem cell-derived hepatocytes from patients with rheumatoid arthritis
... stem cells (iPSCs), which are induced by viruses expressing the Yamanaka factors (OCT4, Sox2, Klf4, c-Myc), can be generated from mul- tiple types of somatic cells, including fibroblasts and per- ...
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