induced osteoblast-like cells
Zirconia Nanoparticles Induced Toxic Effects in Osteoblast Like 3T3 E1 Cells
... h. Cells were washed immediately after the treatment with PBS buffer for three times and fixed with 4% para- formaldehyde, permeabilizated with ...Then, cells were incubated for α-tubulin (Sigma-Aldrich, ...
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Photothermal effects of laser-activated surface plasmonic gold nanoparticles on the apoptosis and osteogenesis of osteoblast-like cells
... kill cells more efficiently by continuous wave laser and how to treat deeper or larger areas of ...cancer cells. Nevertheless, nontarget cells can take up AuNPs through nonspecific ...nontarget ...
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Constitutive and inducible expression of HLA class II determinants by human osteoblast like cells in vitro
... analysis and immunoperoxidase techniques using monoclonal antibodies. A subset of HBC (10-30%) expressed DR constitutively while 5-15% displayed DQ during long-term culture. HBC lacked a number of monocyte and lymphocyte ...
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Modulation of TGFbeta 2 levels by lamin A in U2-OS osteoblast-like cells: understanding the osteolytic process triggered by altered lamins
... and osteoblast metabolism as well as on hematopoietic osteoclast precursors [11, 15, ...is induced by media from R527H-LMNA transfected osteoblast-like cells containing elevated TGFbeta ...
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The effects of zoledronate on the survival and function of human osteoblast like cells
... time-dependently induced apoptosis of osteoblasts after concentrations had reached 10 μ M ( p < ...inhibited osteoblast migration by 50 % were between 10 and 15 μ ...affected osteoblast survival ...
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Neotendon formation induced by manipulation of the Smad8 signalling pathway in mesenchymal stem cells
... decades. It has been shown that adult MSCs are able to differenti- ate into mesenchymal phenotypes including bone-forming osteo- blasts, cartilage-forming chondrocytes, fat-storing adipocytes, and muscle- or ...
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Proliferating and differentiating effects of three different growth factors on pluripotent mesenchymal cells and osteoblast like cells
... human osteoblast like cells and a murine pluripotent mesenchymal cell line, BMP-2 induced cell differentiation, whereas IGF-I and PDGFbb stimulated cell ...factors induced migration in ...
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Phytase mediated enzymatic mineralization of chitosan enriched hydrogels
... human osteoblast-like MG63 cells and adipose tissue- derived stem cells ...Phytase induced CaP ...MG63 cells (with and without ...
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Nanocoating with plant-derived pectins activates osteoblast response in vitro
... bone cells, bone matrix formation, and mineralization at the implant surface by modifying surface coating on the nanoscale ...mice osteoblast-like cells MC3T3-E1 and primary murine ...
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... fibroblast cells and UMR- osteoblas-like cells induced by conven- tional glass-ionomer cements were significantly ...fibroblast cells, sig- nificantly different only from Vitrebond ...
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Biocompatibility of three bioabsorbable membranes assessed in FGH fibroblasts and human osteoblast like cells culture
... Human osteoblast like-cells (SaOs-2) and gingival fibroblasts FGH (BCRJ -RJ) were cultured in DMEM ...the cells cultured on the membranes was assesses using ...
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Initial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule
... In this study, we examined the surface characteristics of NANOZR, Y-TZP and CpTi, and we evaluated the initial response of osteoblast-like cells to them with different inhibitors to elucidate the ...
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Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
... MG-63 cells (Exbio, Prague, Czech Republic) and monoclonal rat anti-mouse ICAM-1 antibody (Exbio Prague, clone ...from cells growing on PLGA and PLGA-ND membranes were expressed as a percentage of the ...
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Factorial study of compressivemechanical properties and primary in vitro osteoblast response of PHBV/PLLA scaffolds
... In our previous studies [5–7], we reported the fabri- cation, in vitro degradation and biological evaluation of PHBV-based sca ff olds using emulsion freezing/freeze-drying process. In the present paper, a factorial ...
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TGF beta 1 and 25 hydroxycholesterol stimulate osteoblast like vascular cells to calcify
... oxysterol 25-hydroxycholesterol as well as by transforming growth factor-beta 1, both known to be present in atherosclerotic lesions. The stimulation of calcifying vascular cells in the artery wall by these two ...
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Topographical and chemical effects of electrochemically assisted deposited hydroxyapatite coatings on osteoblast like cells
... The results shown in this study provide interesting insight into how osteoblast-like cells are affected by the topography of the surface on which they adhere. The needle-like morphology of the ...
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DETECTION OF BETA ACTIN AND OSTEOCALCIN GENES EXPRESSION IN DIFFERENTIATED OSTEOBLASTS BY RT PCR
... Osteoblasts are engaged in bone formation and Osteoblasts are responsible for the production of collagen and non-collagenous proteins including osteocalcin, bone sialoprotein, osteopontin, and osteonectin. [8] Beta-actin ...
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Sostdc1: A soluble BMP and Wnt antagonist that is induced by the interaction between myeloma cells and osteoblast lineage cells
... myeloma cells but not in the bone marrow of non-tumour bearing ...myeloma cells, it should be noted that the bones examined were well infiltrated at the time of sacrifice, a point where there were few visible ...
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Characterisation of extraembryonic endoderm-like cells from mouse embryonic fibroblasts induced using chemicals alone
... ciXEN cells derived from the selected clones. ciXEN cells had two distinct morphological characteristics: dis- persed cells at low density and epithelioid cells at high density ...XEN ...
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Effect of β TCP Size on Bone Like Layer Growth and Adhesion of Osteoblast Like Cells in Hydroxyapatite/β TCP Composites
... experimental results. In the present study, the osteoblast-like cells were cultured only for 2.5 hours; thus the cell death due to the toxicity of calcium was insignificant, resulting in the good cell ...
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