Insulin-degrading enzyme
Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells
... Fig. 2. Protein expression and the activity of insulin-degrading enzyme (IDE) were analyzed in different passages of Caco-2 cell extracts. (A) Three different passage ranges of Caco-2 cells were ...
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Insulin-degrading enzyme is exported via an unconventional protein secretion pathway
... Insulin-degrading enzyme (IDE) is a ubiquitously expressed zinc-metalloprotease that degrades several pathophysiologically significant extracellular substrates, including insulin and the ...
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Original Article The gene polymorphisms of insulin degrading enzyme (IDE) are associated with the risk of coronary heart disease in Chinese Han population
... Altered insulin degrading enzyme function leads to toxic amyloidand insulin aggregates, inflammation, and ...of insulin degrading enzyme (IDE) are associated with the ...
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The Amino Terminus of Varicella-Zoster Virus (VZV) Glycoprotein E Is Required for Binding to Insulin-Degrading Enzyme, a VZV Receptor
... Varicella-zoster virus (VZV) glycoprotein E (gE) is required for VZV infection. Although gE is well conserved among alphaherpesviruses, the amino terminus of VZV gE is unique. Previously, we showed that gE interacts with ...
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Cellular distribution of insulin degrading enzyme gene expression Comparison with insulin and insulin like growth factor receptors
... Insulin-degrading enzyme (IDE) hydrolyzes both insulin and IGFs and has been proposed to play a role in signal termination after binding of these peptides to their ...with insulin ...
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Development of monoclonal antibodies and quantitative ELISAs targeting insulin-degrading enzyme
... Conclusion: We succeeded in developing novel mAbs that selectively detect rodent and/or human IDE, which we have shown to be suitable for a wide range of applications, including western[r] ...
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Statins induce insulin-degrading enzyme secretion from astrocytes via an autophagy-based unconventional secretory pathway
... that IDE levels in the cells were reduced conversely (Fig. 1a, b). Because statins are known to lower choles- terol levels, we checked whether statins regulate choles- terol levels in astrocytes. By using a filipin ...
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Insulin-Degrading Enzyme Binds to the Nonglycosylated Precursor of Varicella-Zoster Virus gE Protein Found in the Endoplasmic Reticulum
... degradation enzyme (IDE) is a 110-kDa zinc metalloprotease found in the cytosol of all ...degrades insulin and a variety of small proteins including amyloid-  ...
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Involvement of insulin-degrading enzyme in the clearance of beta-amyloid at the blood-CSF barrier: Consequences of lead exposure
... IDE, a 110-KD zinc metallopeptidase, is known to partic- ipate extensively in the clearance of insulin, glucagon and Aβ [17-21]. The common physicochemical characteristics shared by IDE substrates include ...
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Direct measurement of proinsulin in human plasma by the use of an insulin degrading enzyme
... this enzyme rapidly degrades insulin and insulin-like polypeptides to nonimmunoassayable components, whereas proinsulin and proinsulin cleaved at position B 54,55 are not appreciably ...of ...
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Ralstonia solanacearum Type III Effector RipAY Is a Glutathione Degrading Enzyme That Is Activated by Plant Cytosolic Thioredoxins and Suppresses Plant Immunity
... GSH degradation assay. GSH (10 mM) was incubated with 3g of the RipAY or YER163c protein for 60 min at 30°C in 100 l of reaction mix- ture containing 50 mM Tris-HCl (pH 8.0). The reaction mixture was then incubated at ...
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Discovery and exploitation of the transcriptional regulatory system of pectinases in Aspergillus niger
... The enzyme families controlled by orthologous transcription factors can ...CAZymes degrading pectin, especially PGA, has been identified in both Botrytis cinerea and ...
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Screening of SDS-degrading bacteria from car wash wastewater and study of the alkylsulfatase enzyme activity.
... Preparation of enzyme extracts. Bacterial cells were harvested at day 2 by centrifugation of a 12 ml culture at 10,000 × g for 20 min at 4°C in a Beckman J20 high-speed centrifuge. Cell pellets were resuspended in ...
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Expression of the Clonostachys rosea lactonohydrolase gene by Lactobacillus reuteri to increase its zearalenone-removing ability
... of enzyme supplementation because the genes for these enzyme genes could be replicated and expressed by the probiotics within the digestive tract ...ZEN-degrading enzyme by intestinal ...
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Bioconversion of Cellulosic Materials by the action of Microbial Cellulases
... cellulase enzyme: Cellulase is an important enzyme for carrying out the depolymerization of cellulose into fermentable ...this enzyme in so many products has raised considerable interest and research ...
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Screen a dehalogenase gene using isolated bacterium from sea-shore soil of caspian sea
... of degrading and utilizing 2, 2-DCP by dehalogenase enzyme (Kearney et ...of degrading 2,2-dichloropropionic acid as sole sources of carbon and ...
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The Phytophthora infestans haustorium is a site for secretion of diverse classes of infection associated proteins
... INF4 and PE1 are conventionally secreted from haustoria. To localize PE1-mRFP and INF4-mRFP during infection, they were transformed into P. infestans. The native signal peptides for secretion were retained. Free green ...
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The immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis , is involved in complement evasion
... In this study we obtained in vitro data supporting the hypothesis that Ide Ssuis is involved in complement evasion: (i) different recombinant Ide Ssuis constructs abolished the hemolysis[r] ...
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CURCUMIN, NORDIHYDROGUIARETIC ACID, QUERCETIN AND RESVERATROL INHIBIT INTERLEUKIN-1-INDUCED ADAMTS-4 (AGGRECANASE-1) GENE EXPRESSION IN ARTICULAR CHONDROCYTES:
... aggrecan-degrading enzyme expression can be inhibited by the active plant-originated natural products such as Curcumin, NDGA, Quercetin and Resveratrol in human articular ...
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Degradation of blood group antigens in human colon ecosystems II A gene interaction in man that affects the fecal population density of certain enteric bacteria
... group- degrading enzyme activity after 24 h incubation in anaerobic ...antigen-degrading enzyme activities were comparable in all subjects to the ...
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