Insulin mRNA
Hypoxia May Increase Rat Insulin mRNA Levels by Promoting Binding of the Polypyrimidine Tract-binding Protein (PTB) to the Pyrimidine-rich Insulin mRNA 3′-Untranslated Region
... that insulin mRNA is highly abundant and constitutes up to 30% of the pancreatic beta cell mRNA ...The insulin mRNA is also especially long-lived, 29–77 hr, depending on glucose ...
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Preservation of insulin mRNA levels and insulin secretion in HIT cells by avoidance of chronic exposure to high glucose concentrations
... of insulin secretion associated with serial passages of HIT-T15 cells might be caused by chronic exposure to high glucose levels since these cells are routinely cultured in media containing supramaximal ...
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Effects of tacrolimus (FK506) on human insulin gene expression, insulin mRNA levels, and insulin secretion in HIT T15 cells
... of insulin gene expression in the beta cell may also involve Ca2+-signaling pathways and FK506 has been associated with insulin-requiring diabetes mellitus during clinical ...human insulin gene ...
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Release of insulin produced by the choroid plexis is regulated by serotonergic signaling
... RNAscope FISH. Insulin mRNA in brain sections was detected by FISH. RNAscope probes for mouse Ins2 and Iapp was customer designed (Ins2: 10 ZZ pairs targeting 2–470 nucleotide sequence of NM_008387.5 named ...
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Differentiation of glucose toxicity from beta cell exhaustion during the evolution of defective insulin gene expression in the pancreatic islet cell line, HIT T15
... expression. Insulin mRNA levels in HIT cells cultured under the four experimental conditions were com- pared by both Northern blot and slot blot analysis to deter- mine whether the observed decline in ...
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Adaptation to supraphysiologic levels of insulin gene expression in transgenic mice: evidence for the importance of posttranscriptional regulation
... human insulin, showed that the excess insulin mRNA was due solely to expression of the foreign, human insulin ...of mRNA for mouse insulin I and II was unaffected by coexpression ...
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Effects of hypoglycemia and prolonged fasting on insulin and glucagon gene expression Studies with in situ hybridization
... 12-d insulin infusion blood glucose averaged 53 +/- 12 mg/dl; proinsulin mRNA signal density declined to 30% of controls while proglucagon mRNA signal density more than ...proinsulin mRNA ...
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Molecular adaptations of adipose tissue to 6 weeks of morning fasting vs daily breakfast consumption in lean and obese adults
... and insulin concentrations; composite Matsuda insulin sensitivity index, adipose tissue glucose uptake rates expressed per milligram lipid) (Betts et ...
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The role of insulin as a key regulator of seeding, proliferation, and mRNA transcription of human pluripotent stem cells
... of phosphorylation led to decrease in G1 phase accom- panying an increase in apoptosis or decrease in prolifera- tion in these cells [10, 13]. This reduction in percentage of cells in the G1 phase alongside a decrease in ...
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Glycogen synthase and phosphofructokinase protein and mRNA levels in skeletal muscle from insulin resistant patients with non insulin dependent diabetes mellitus
... nonoxidative glucose metabolism was decreased by 47% (P < 0.005) in NIDDM patients, whereas the glucose oxidation rate was normal. The PFK activity, protein level, and mRNA/microgram DNA remained unchanged. The ...
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Maternal Magnesium Deficiency in Mice Leads to Maternal Metabolic Dysfunction and Altered Lipid Metabolism with Fetal Growth Restriction
... elongase mRNA expression in maternal livers along with higher circulating insulin and glucose concentrations (P < ...creased mRNA expression of Srebf1 and Chrebp, regulators of fatty acid ...
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Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) in cancer
... The insulin-like growth factor-2 mRNA-binding protein 1 (IGF2BP1) plays essential roles in embryogenesis and ...essential mRNA targets required for the control of tumor cell proliferation and growth, ...
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A calpain 10 gene polymorphism is associated with reduced muscle mRNA levels and insulin resistance
... CAPN10 mRNA (and β-actin mRNA as an endogenous refer- ence) was done as a real-time measure- ment in an ABI PRISM 7700 Sequence Detection System (Applied Biosystems ...
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Detection of amplifiable mRNA extracellular to insulin producing cells: potential for predicting beta cell mass and function
... and mRNA of fetal origin have been discovered in the plasma of pregnant women (7–9 ), and significantly increased concentrations of rhodopsin mRNA have been found in the peripheral blood of individuals with ...
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Hyperinsulinemia is associated with altered insulin receptor mRNA splicing in muscle of the spontaneously obese diabetic rhesus monkey
... human insulin receptor has two isoforms derived from alternative splicing of exon 11 of the insulin receptor ...binds insulin with twofold lower affinity than the type A (lacking exon 11, or exon ...
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Bürck, Lelia van (2010): Clinical and pathomorphological characterisation of two diabetic mouse models derived from the Munich ENU mouse mutagenesis project. Dissertation, LMU München: Tierärztliche Fakultät
... glucokinase mRNA expression seems to be more constitutive, enabling the stable regulation of glucose turnover and insulin secretion rates in pancreatic β-cells (Matschinsky et ...glucokinase mRNA ...
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Clear cell carcinomas of the ovary: a mono-institutional study of 73 cases in China with an analysis of the prognostic significance of clinicopathological parameters and IMP3 expression
... The present study aimed to evaluate the prognostic significance of its clinicopathological characteristics and the expression of IMP3 (Insulin-like growth factor-II mRNA-binding protein [r] ...
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Modulation of proinsulin messenger RNA after partial pancreatectomy in rats Relationships to glucose homeostasis
... Proinsulin mRNA was measured by dot hybridization to complementary ...proinsulin mRNA. After 90% Px a marked increase of proinsulin mRNA occurred, but it was insufficient and it was not maintained ...
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Feedback inhibition of insulin gene expression by insulin
... that insulin has a negative feedback inhibitory effect on its own ...of insulin on its own transcription, or alternatively an indirect effect mediated by humoral or neural ...
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Neuroendocrine regulation of feeding behaviour
... Five G-protein coupled NPY receptors have been identified – Y1, Y2, Y4, Y5 and Y6.Y5 receptors have been implicated as important receptors that mediate the feeding effects of NPY.The Y5 receptor is expressed at ...
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