Integral membrane proteins
Structural studies of large integral membrane proteins in reverse micelles by solution nuclear magnetic resonance
... of membrane protein NMR structures to the relatively small β- barrel proteins or helical proteins of simple ...of integral membrane proteins in reverse micelles, allowing for ...
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Yeast Integral Membrane Proteins Apq12, Brl1, and Brr6 Form a Complex Important for Regulation of Membrane Homeostasis and Nuclear Pore Complex Biogenesis
... Microscopy. The micrographs in Fig. 1 were recorded using a Nikon TE2000-E microscope (Nikon 100 ⫻ Plan Apochromat oil objective; nu- merical aperture [NA], 1.4), an Orca-ER charge-coupled-device (CCD) camera ...
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Are plant formins integral membrane proteins?
... (FH) proteins, are proteins implicated in cellular and organismal morphogene- sis of both metazoa and ...[12,13], proteins containing SH3 or WW domains [10,14], dynein and microtubules ...
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Protein Targeting to the Parasitophorous Vacuole Membrane of Plasmodium falciparum
... ated, integral membrane proteins, EXP1, Pfs16, and the ETRAMP family proteins are small proteins with a predicted secretory signal sequence, followed by a lysine-rich region and then a ...
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The Integral Membrane Protein Pom34p Functionally Links Nucleoporin Subcomplexes
... pore membrane proteins, we have conducted experiments to define the Pom34p membrane topology and ad- dress the Pom34p functional relationship with ...brane proteins in the structural ...
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Voltage-Gated Potassium Channels Kv1.3 –Potentially New Molecular Target in Cancer Diagnostics and Therapy
... are integral membrane proteins which are activated (“open”) upon change of the cell membrane potential, enabling a passive flux of potassium ions across the cell ...plasma membrane, but ...
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Region E3 of subgroup B human adenoviruses encodes a 16-kilodalton membrane protein that may be a distant analog of the E3-6.7K protein of subgroup C adenoviruses.
... of integral membrane proteins; consistent with this, we detected 16K in the crude membrane but not the cytosol cellular ...Asn-linked integral membrane glycoprotein, localized in ...
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Constitutive endocytosis of GABA(A) receptors by an association with the adaptin AP2 complex modulates inhibitory synaptic currents in hippocampal neurons
... integral membrane proteins into clathrin-coated pits. GABA A receptors also colocalize with AP2 in cultured hippocampal neu- rons. Finally, blocking clathrin-dependant endocytosis with a peptide that ...
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Isolation of proteins related to the Rh polypeptides from nonhuman erythrocytes
... It is thought that the Rh antigens may be important in maintaining normal erythrocyte membrane integrity. Despite their name, Rh antigens are serologically present only on human erythrocytes. Rh structural ...
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The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae
... SH proteins of different ...SH proteins of SV5 and hRSV are type II integral membrane proteins which are packaged in virions (9, 10, 17, ...G proteins of members of the ...
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RNA Replication of Mouse Hepatitis Virus Takes Place at Double-Membrane Vesicles
... the integral membrane proteins remain associated under these ...the membrane-associated products and thus was specific to the ...the membrane association of ...an integral ...
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Strategies for the purification of membrane proteins
... Membrane proteins are classified into peripheral and integral membrane proteins, which are associated to varying degrees with the phospholipid bilayer (6, ...extrinsic membrane ...
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Strategies for the purification of membrane proteins
... solubilize proteins by binding to the hydrophobic parts of the protein on one side and interacting with the aqueous parts on the other side ...the membrane protein without irreversibly denaturing ...of ...
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Abstract: In order to alter and adjust the shape of the membrane, cells harness various mechanisms of curvature
... the membrane, cells harness various mechanisms of curvature ...peripheral membrane proteins, integral membrane proteins, and lipids in the bilayer ...the membrane free ...
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Bead reagents for membrane proteins.
... globular proteins, either membrane- associated or free in solution, it is probable that even functional group densities of 2 y^eq/g are excessive with the beads presently ...
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Chapter 7 Lecture.ppt
... Fibers of extracellular matrix (ECM) Glyco- protein Microfilaments of cytoskeleton Cholesterol Peripheral proteins Integral protein CYTOPLASMIC SIDE OF MEMBRANE Glycolipid EXTRACELLULAR [r] ...
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Yeast mitochondrial membrane proteins
... Before chloroform-methanol extraction, the proteins were precipitated with NH 4 2 S0 4 • Experimental variation Standard procedure SOS concentration between 2-5% during sample dissociat[r] ...
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Role of Receptor-Mediated Endocytosis in the Formation of Vaccinia Virus Extracellular Enveloped Particles
... viral membrane proteins from the plasma mem- brane to early endosomes could be crucial if the latter form the wrapping ...viral proteins from the plasma membrane could be important even if the ...
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A versatile strategy for production of membrane proteins with diverse topologies: application to investigation of bacterial homologues of human divalent metal ion and nucleoside transporters
... To facilitate the rapid screening of expression constructs bearing different affinity tags, and to compare the use of different promoters, we cloned target ORFs, flanked by suitable restriction sites, into the vector ...
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A 12-Residue-long Polyleucine Tail Is Sufficient to Anchor Synaptobrevin to the Endoplasmic Reticulum Membrane
... cient to anchor Syb2 in the ER membrane. A, Syb2-17L (which has 17 leucines and 1 valine in the hydrophobic segment) was translated in vitro either in the absence (lanes 1 and 5) or presence (lanes 2– 4 and 6 – 8) ...
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