Interferon regulatory factor 5 (IRF5)
Interferon regulatory factor 5 genetic variants are associated with cardiovascular disease in patients with rheumatoid arthritis
... risk. Interferon regulatory factor 5 (IRF5) is a regulator of type I interferon ...the IRF5 gene and some rheumatic ...the IRF5 locus which have been shown to alter ...
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<p>Transcription factor E2F1 positively regulates interferon regulatory factor 5 expression in non-small cell lung cancer</p>
... IRF5, Interferon regulatory factor 5; E2F1, E2F transcrip- tion factor 1; NSCLC, Non-small cell lung cancer; LC, lung cancer; IRF, interferon regulatory factors; ...
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Interferon Regulatory Factor 5 Represses Expression of the Epstein-Barr Virus Oncoprotein LMP1: Braking of the IRF7/LMP1 Regulatory Circuit
... (Fig. 5, pathway ...(Fig. 5, pathways 2 and 3), which may help to explain why IRF7 is expressed at appreciable levels in type II nasopharyngeal carcinoma tissues ...positive regulatory circuit be- ...
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Interferon-Regulatory Factor 5-Dependent Signaling Restricts Orthobunyavirus Dissemination to the Central Nervous System
... in Irf5 ⫺/⫺ mice (9, 11, 34), we hypothe- sized that IRF-5 might be required for optimal adaptive immunity against ...and Irf5 ⫺/⫺ mice (Fig. 11A and B). Irf5 ⫺/⫺ mice had only slightly lower ...
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Loss of interferon regulatory factor 5 (IRF5) expression in human ductal carcinoma correlates with disease stage and contributes to metastasis
... to IRF5-mediated apoptosis or necrosis, we measured AnnV-FITC and PI-double staining by flow ...cells, IRF5 provided an approximately 40% increase in positive-stained ...with IRF5 provided a syner- ...
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IRF5 governs liver macrophage activation that promotes hepatic fibrosis in mice and humans
... for 5 minutes at 40 g; the resulting pellet composed the hepatocyte fraction, and the supernatant was composed of ...for 5 minutes at 500 g; supernatant was removed, and pellet was snap-frozen in liquid ...
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Rotavirus NSP1 Inhibits Expression of Type I Interferon by Antagonizing the Function of Interferon Regulatory Factors IRF3, IRF5, and IRF7
... is interferon (IFN) regulatory factor 3 (IRF3), one of the nine structurally related members of the IRF family that, as a group, exhibit a broad range of functions in regulat- ing the immune system ...
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Interferon Regulatory Factor 3 Is a Cellular Partner of Rotavirus NSP1
... gene 5 that result in the synthesis of truncated NSP1 have been isolated from animals and from both immune-deficient and immune-competent children (1, 7, 15, 25, 35, 44, ...
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Kaposi’s Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor
... Cloning of KSHV K9 from BCBL-1 cells. The KSHV K9 open reading frame includes bp 86074 to 88164 of the published sequence of KSHV (28). A DNA fragment corresponding to the KSHV K9 was synthesized from BCBL-1 genomic DNA ...
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Original Article Regulatory effect of interferon regulatory factor-1 on lymphatic enhancing factor 1
... IRF-1 siRNA (5’-GATAG ACCTA ATGGA TAG-3’) or scrambled control oligonucleotide (5’-ACCTA CTTCC GTAAT GT-3’) was transfected into HCT116 cells. In brief, cells were cultured in 6-well plate until reaching ...
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Distinct Roles of Kaposi's Sarcoma-Associated Herpesvirus-Encoded Viral Interferon Regulatory Factors in Inflammatory Response and Cancer
... viral interferon (IFN) regulatory factor 3 ...lar interferon regulatory factors (IRFs) that play a critical role in type I IFN gene induction and IFN ...
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Interferon regulatory factor 8/interferon consensus sequence binding protein is a critical transcription factor for the physiological phenotype of microglia
... Microglia, the resident mononuclear phagocyte (MP) sys- tem of the CNS, play principal roles in the innate immune response to tissue damage and invasion of foreign organ- isms, and also in initiation and modulation of ...
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Rapid Upregulation of Interferon-Regulated and Chemokine mRNAs upon Injection of 108 International Units, but Not Lower Doses, of Adenoviral Vectors into the Brain
... the interferon (IFN)-regulated proteins interferon regulatory factor 1, 2 ⴕ ,5 ⴕ -oligoadenylate synthetase, and double-stranded-RNA-dependent protein kinase as well as those coding for ...
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Inhibition of p53 Tumor Suppressor by Viral Interferon Regulatory Factor
... mary of GST-vIRF fusion constructs and in vitro GST pull-down assay. Individual domains of vIRF were cloned into the pGEX4T-1 vector to generate GST-vIRF fusion proteins. Lysates of Saos-2 cells infected with Ad-p53 were ...
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HIV-1 Vpu Does Not Degrade Interferon Regulatory Factor 3
... To exclude the possibility that some subtle effects of Vpu on IRF-3 were missed because the cells were examined in bulk, we next performed fluorescence-activated cell sorting (FACS)-based assays. The pCG vectors used in ...
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Inhibition of Beta Interferon Induction by Severe Acute Respiratory Syndrome Coronavirus Suggests a Two-Step Model for Activation of Interferon Regulatory Factor 3
... IRF-3 nuclear translocation assay. Endogenous IRF-3 can be detected in Vero cells by enzymatic amplification of the immunofluorescence signal (32). Cells were grown on coverslips and infected with SARS-CoV at a ...
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Interferon regulatory factor-7 modulates experimental autoimmune encephalomyelitis in mice
... animal’s slowness or splaying limbs when walking or unsteady walk in the cage or on the lid of the cage), Grade 4: 3 + very weak hind limbs or one hind limb paralysed- hind limb paresis-assessed by the animal dragging ...
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Arenavirus Nucleoprotein Targets Interferon Regulatory Factor-Activating Kinase IKKε
... The specific ability of arenavirus NP to target IKKε, but not TBK-1, was unexpected, because existing evidence indicates a more important role for TBK-1 in the induction of type I IFNs in response to virus infection (16, ...
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Identification of TRAIL as an Interferon Regulatory Factor 3 Transcriptional Target
... similar to electrophoretic mobility shift assays but use an en- zyme-linked immunosorbent assay-based format. We prepared nuclear extracts from HT-29 cells that were either untreated or SeV infected. Extracts were ...
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Virus-Activated Interferon Regulatory Factor 7 Upregulates Expression of the Interferon-Regulated BST2 Gene Independently of Interferon Signaling
... V5V cells unresponsive to IFN- ␣ -induced upregulation of surface BST-2 (Fig. 7A, bottom panel). IRF1, IRF3c, or IRF7c was tran- siently coexpressed with green fluorescent protein (GFP) in HEK293T or HEK293T-V5V cells ...
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