Intracellular pH regulation
Preferential intracellular pH regulation: hypotheses and perspectives
... base regulation is an essential physiological process that demands tight ...physiological pH values differ between species and between body compartments within species, and are affected by temperature ...
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Intracellular pH regulation of CA1 neurons in Na+/H+ isoform 1 mutant mice
... This transforms the surrounding sequence from CAA- CAAGTTCC to CAACTAGTTCC. Spe1, which cuts between A and C in the sequence ACTAG, cleaves the mutant molecule (2 bands) but not the WT (1 band). Statistics. Data are ...
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A vacuolar type H+ ATPase and a Na+/H+ exchanger contribute to intracellular pH regulation in cockroach salivary ducts
... The epithelial cells forming the ducts in the acinar salivary glands of the cockroach Periplaneta americana have a V- ATPase in their highly folded apical membrane (Just and Walz, 1994a) but its functional significance ...
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Short Communication: Fish Hepatocytes Accumulate Hepes: A Potential Source of Error in Studies of Intracellular pH Regulation
... Assuming that a mean of these values 67% is approximately correct for toadfish hepatocytes, then l.OSmmolP 1 Hepes accumulation at lOmmolP 1 external Hepes concentration would increase t[r] ...
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Intracellular pH regulation and proton transport by rabbit renal medullary collecting duct cells Role of plasma membrane proton adenosine triphosphatase
... absence of glucose inhibited pHi recovery. ATP depletion inhibited proton extrusion from MCD cells. Treatment with N-ethylmaleimide also inhibited pHi recovery. In addition, cellular ATP content was dependent on ...
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Effect of secretion on intracellular pH regulation in isolated rat bile duct epithelial cells
... BDE cells preincubated and perfused with 100 MM DBcAMP D, n = 11, in presence of HCO3- KRB, showed a maximal rate of alkalinization after Cl- removal gluconate and of pHi recovery after [r] ...
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Intracellular pH regulation in unstimulated Calliphora salivary glands is Na+ dependent and requires V ATPase activity
... this transporter with DIDS is expected to cause an intracellular acidification. Such an acidification was indeed observed in the presence of DIDS in C. vicina salivary glands in the present study, although this ...
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Intracellular pH regulation in rabbit renal medullary collecting duct cells Role of chloride bicarbonate exchange
... The renal medullary collecting duct (MCD) secretes protons into its lumen and HCO3 into its basolateral space. Basolateral HCO3 transport is thought to occur via Cl/HCO3 exchange. To further characterize this Cl/HCO3 ...
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Intracellular pH regulation in isolated rat bile duct epithelial cells
... recovery was inhibited by DIDS (0.5-1 mM), but not by Cl- depletion. Finally, acute Cl- removal increased pHi by 0.18 pH units in the absence but not presence of DIDS. These data indicate that BDE cells possess ...
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Effect of glucagon on intracellular pH regulation in isolated rat hepatocyte couplets
... To elucidate mechanisms of glucagon-induced bicarbonate-rich choleresis, we investigated the effect of glucagon on ion transport processes involved in the regulation of intracellular pH (pHi) in ...
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The Role of Physico Chemical Buffering and of Bicarbonate Transfer Processes in Intracellular pH Regulation in Response to Changes of Temperature in the Larger Spotted Dogfish (Scyliorhinus Stellaris)
... In order to evaluate the contributions of physico-chemical buffering to the adjustment of intracellular pH in response to changes of temperature in tissues of dogfish Scyliorhinus stella[r] ...
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The interaction between metabolism and the plasma membrane potential, and intracellular pH
... or intracellular receptor and these are called ligand-gated ion channels such as GABA receptors or the P2x family of ATP receptors ...an intracellular substance, such as Ca 2+ -gated potassium channels, in ...
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Intracellular convection, homeostasis and metabolic regulation
... an intracellular convection system acts as an over-riding ‘assist’ mechanism for facilitating enzyme–substrate ...that intracellular movement and convection are powered by macromolecular motors ...
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Control and modulation of intracellular pH in mammalian central neurones
... underestimation of the acid load would lead to an overestimation of buffering power (consider equation 2; h=[Aacid]/ApH;). To overcome this problem buffering power was estimated in the presence of a MCT inhibitor, ...
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Intracellular Acid Base Regulation II The Interaction between CO2 Tension and Extracellular Bicarbonate in the Determination of Muscle Cell pH
... The lower curve in Figure 2 shows only those experiments at an elevated extracellular [HCO3-] in which the pH of the medium was above normal, and hence this curve can be considered descr[r] ...
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THE RELATIONSHIP BETWEEN INTRACELLULAR pH AND SWIMMING PERFORMANCE OF BROWN TROUT EXPOSED TO NEUTRAL AND SUBLETHAL pH
... Galloway (Harriman et al. 1987), mid Wales and north Wales (Turnpenny et al. 1987). Titanium cooling coils and small aquarium heaters maintained the temperature of the water in the two large holding tanks at 15˚C (June ...
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Water pH limits extracellular but not intracellular pH compensation in the CO2 tolerant freshwater fish Pangasianodon hypophthalmus
... Pangasianodon hypophthalmus (Sauvage 1878) were obtained from a local fish supplier in Can Tho, Vietnam and kept at Can Tho University for 3 months prior to experimentation. Fish were held in aerated 3000 l tanks fitted ...
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Renal acid excretion and intracellular pH in salt sensitive genetic hypertension
... Acid-base status and renal acid excretion were studied in the Dahl/Rapp salt-sensitive (S) rat and its genetically salt-resistant counterpart (R). S rats developed hypertension while on a very high salt diet (8%) and ...
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Intracellular pH homeostasis and serotonin induced pH changes in Calliphora salivary glands: the contribution of V ATPase and carbonic anhydrase
... of intracellular [cAMP] by bath application of cAMP, 8-CPT-cAMP, forskolin or IBMX produces a similar intracellular acidification as 5-HT and, as shown here for 8-CPT-cAMP, a concanamycin A-sensitive ...
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Intracellular pH during "chemical hypoxia" in cultured rat hepatocytes Protection by intracellular acidosis against the onset of cell death
... extracellular pH (pHo), intracellular pH (pHi), and loss of cell viability were evaluated in cultured rat hepatocytes after ATP depletion by metabolic inhibition with KCN and iodoacetate (chemical ...
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