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IPTG, isopropyl-β-thiogalactopyranoside

Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

... which isopropyl β-d-1- thiogalactopyranoside (IPTG) is the most popular choice and widely applied, especially in lab ...of IPTG and the time when it is added during cultivation are ...

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Cheese whey-induced high-cell-density production of recombinant proteins in Escherichia coli

Cheese whey-induced high-cell-density production of recombinant proteins in Escherichia coli

... as IPTG (isopropyl-β-D-thiogalactopyranoside) or pure ...to IPTG-induction can be achieved by feeding bacteria with a mixture of glycerol and concentrated whey permeate during the ...

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Prokaryotic Expression of the Oligopeptide Transporter hPepT2(560-663)

Prokaryotic Expression of the Oligopeptide Transporter hPepT2(560-663)

... BL21(DE3), isopropyl β-D- 1-thiogalactopyranoside (IPTG), restriction endonucleases NdeI and BamH I, lysozyme, and T4 DNA ligase were all purchased from TaKaRa Biotechnology ...

5

Selective Membrane Permeabilization by the Rotavirus VP5* Protein Is Abrogated by Mutations in an Internal Hydrophobic Domain

Selective Membrane Permeabilization by the Rotavirus VP5* Protein Is Abrogated by Mutations in an Internal Hydrophobic Domain

... induced proteins to permeabilize cells to ONPG was compared by measuring ONPG cleavage within cells as previously de- scribed (26, 32). To account for differences in the expression of VP4, VP8*, and VP5* proteins from ...

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Analysis and Characterization of Isopropyl Carbanilate Herbicide and Its Photoproducts

Analysis and Characterization of Isopropyl Carbanilate Herbicide and Its Photoproducts

... The phototransformation of the herbicide Isopropyl carbanilate (IPC) has been investigated under UV light. Irradiation of the herbicide at room temperature in aqueous and organic solvents such as hexane and metha- ...

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Erroneously High Dextrostix Values Caused by Isopropyl Alcohol

Erroneously High Dextrostix Values Caused by Isopropyl Alcohol

... The 70% isopropyl alcohol sample had no apparent visible color change of the glucose oxidase peroxidase chromogen reagent, yet resulted in a glucose inter- pretation of 32 mg/100 ml by t[r] ...

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The Role of Isopropyl Alcohol in the Properties of Sudanese Reformat Gasoline

The Role of Isopropyl Alcohol in the Properties of Sudanese Reformat Gasoline

... The addition of isopropyl alcohol with different concentrations raises the value of the octane number after the distillation and refining processes. Therefore, the distillation test gives an interpretation about ...

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High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG

High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG

... of IPTG from BL21(DE3) cells cultured in LB medium can lead to significantly higher membrane protein production yields than when IPTG is ...of IPTG cultures stably produce membrane proteins in the ...

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Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis

Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis

... mM IPTG was added, and samples were with- drawn at t = 0 (immediately before addition of IPTG) and 2 and 4 h ...heat-stable β-galactosidase (BgaB) as reporter gene ...inducer IPTG remained low ...

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Oxidation of isopropyl alcohol by tripropylammonium fluorochromate:
A kinetic and mechanistic study

Oxidation of isopropyl alcohol by tripropylammonium fluorochromate: A kinetic and mechanistic study

... of isopropyl alcohol by Tripropylammonium fluorochromate {TPAFC} has been Studied spectrophotometrically in presence of Sulphuric acid in aqueous acetic acid medium ...to isopropyl alcohol, TPAFC and ...

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Direct measurements of IPTG enable analysis of the induction behavior of E. coli in high cell density cultures

Direct measurements of IPTG enable analysis of the induction behavior of E. coli in high cell density cultures

... Transitions between the non-induced and induced states were interpreted by Cohn & Horibata in their experiments with the lac operon. From their results, theoretical studies showed that hysteresis is an inherent ...

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EFFECTS IN MAN OF THE ANTICHOLINESTERASE COMPOUND SARIN (ISOPROPYL METHYL PHOSPHONOFLUORIDATE)

EFFECTS IN MAN OF THE ANTICHOLINESTERASE COMPOUND SARIN (ISOPROPYL METHYL PHOSPHONOFLUORIDATE)

... Rate of restoration of plasma and red blood cell terase to near zero, the activity of this enzyme cholinesterase activity was restored at a rate of approximately 1.1 per Following the or[r] ...

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Performance and Emission Characteristics of S I  Engine with Oxygenates

Performance and Emission Characteristics of S I Engine with Oxygenates

... and isopropyl alcohol (IPA) were blended with-gasoline on different volume basis (10%, 20%, 30%) and tested for its performance, and emission characteristics on a single cylinder four stroke spark ignition engine ...

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Synthesis & Characterization of Pectin - N-isopropyl acrylamide modified graft co-polymers for potential Applications

Synthesis & Characterization of Pectin - N-isopropyl acrylamide modified graft co-polymers for potential Applications

... In our study, we have grafted N-isopropyl acrylamide on pectin to improve its properties like gel strength, film forming ability, pH sensitive behavior, thermal sensitivity and to make it more shear stable so that ...

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Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway

Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway

... mM IPTG) resting cells of each of the recombinant strains and a host control (Table 1) were incubated in phosphate buffer with 2 mM TCP, and the time-courses of TCP biotransformation over a 5 h inter- val were ...

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Synthesis and Chiral Separation of Fibratol, Isopropyl 2 (4 ((4 chlorophenyl)(hydroxyl) methyl) phenoxy) 2 methylpropanoate

Synthesis and Chiral Separation of Fibratol, Isopropyl 2 (4 ((4 chlorophenyl)(hydroxyl) methyl) phenoxy) 2 methylpropanoate

... Practical synthetic route for the formation of enantiomeric mixture of Iso- propyl 2-(4-((4-chlorophenyl)(hydroxyl)methyl)phenoxy)-2-methylpropanoate (Fibratol 2a/b) from isopropyl ...

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Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium

Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium

... The Lac repressor (LacI) from E. coli is a well-studied tetrameric (a dimer of dimers) transcription factor [9] or- thogonal to cyanobacteria that represents a model system and, in combination with an optimized promoter ...

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Isopropyl Alcohol Compared with Isopropyl Alcohol plus Povidone Iodine as Skin Preparation for Prevention of Blood Culture Contamination

Isopropyl Alcohol Compared with Isopropyl Alcohol plus Povidone Iodine as Skin Preparation for Prevention of Blood Culture Contamination

... and isopropyl alcohol has been traditionally used in many institutions, the application of povidone-iodine needs extra time, and there is little evidence that this combination could have an additive effect in ...

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Vaccinia Virus 15-Kilodalton (A14L) Protein Is Essential for Assembly and Attachment of Viral Crescents to Virosomes

Vaccinia Virus 15-Kilodalton (A14L) Protein Is Essential for Assembly and Attachment of Viral Crescents to Virosomes

... Early stages in vaccinia virus (VV) assembly involve the recruitment of cellular membranes from the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) to virus factories (or virosomes). The key viral factors ...

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Insights into the structure and activity of prototype foamy virus RNase H

Insights into the structure and activity of prototype foamy virus RNase H

... coli: Escherichia coli; FV: foamy virus; GB1: immunoglobulin binding domain B1 of streptococcal protein G; HIV-1: human immunodeficiency virus type 1; IN: integrase; IPTG: isopropyl-thio[r] ...

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