iTRAQ analysis
An iTRAQ-based proteomic analysis reveals dysregulation of neocortical synaptopodin in Lewy body dementias
... (iTRAQ) analysis of pooled lysates from the prefrontal cortex (BA9) of DLB ( n = 19) and PDD ( n = 21) patients matched a priori for amyloid (total A β 42) burden, semi-quantitative scores for Lewy bodies ...
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Proteomic analysis of elite soybean Jidou17 and its parents using iTRAQ-based quantitative approaches
... The same samples used in the iTRAQ analysis were also used for a western blot analysis. Five differentially expres- sion proteins were selected, and equal amounts of the pro- tein samples from ...
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Potential tumor biomarkers identified in ovarian cyst fluid by quantitative proteomic analysis, iTRAQ
... as iTRAQ and TMT provide the ad- vantages of enabling samples to be mixed into one reac- tion and several samples (up to seven) run together with a reference sample under identical ...an iTRAQ ...
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iTRAQ Proteins Analysis of Early Infected Papaya Plants with Papaya Dieback Pathogen
... spectrometry analysis using iTRAQ reagent For iTRAQ analysis, proteins were dissolved in iTRAQ dissolution buffer and precipitated using ITSI Bio’s ToPREP kit to remove iTRAQ ...
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ITRAQ-based quantitative proteomic analysis of processed Euphorbia lathyris L. for reducing the intestinal toxicity
... were iTRAQ eight plex peptide-labeled, trypsin digestion with only two maximum miss cleavage, carboxymate for cyst- eine residue and oxidation for ...the analysis (where P-value < ...by iTRAQ ...
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Comparative proteome analysis of human epithelial ovarian cancer
... in a spot from which multiple protein identifications were made (protein co-migration) are still common issues. The protein dataset generated by the 2DE analysis is generally limited to high abundant proteins in ...
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The response to chemical stress: development of preclinical and translational biomarkers of Nrf2 activity
... from iTRAQ analysis 1 identified 108 proteins that were differentially expressed in the livers of WT and Nrf2 KO ...MetaCore analysis was used in order to identify the biochemical pathways that were ...
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The rat striatum responds to nigro-striatal degeneration via the increased expression of proteins associated with growth and regeneration of neuronal circuitry
... this, iTRAQ analysis of the striatal proteome at 3 days post lesioning suggests that the expression of cer- tain synaptic proteins ...blot analysis of DARPP-32, a protein long known to be associated ...
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Applying mass spectrometry based proteomic technology to advance the understanding of multiple myeloma
... used iTRAQ analysis to identify differentially expressed proteins between the 8226/R5 bortezomib resistant multiple myeloma cell line and the 8226/S bortezomib sensitive multiple myeloma cell ...systems ...
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Identification of HSPA8 as a candidate biomarker for endometrial carcinoma by using iTRAQ-based proteomic analysis
... ics analysis, we identified a list of proteins that might be closely associated with endometrial carcinoma, including CCT7, HSPA8, PCBP2, LONP1, PFN1, and ...
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Quantitative proteomic analysis by iTRAQ® for the identification of candidate biomarkers in ovarian cancer serum
... DIGE analysis of immunode- pleted sera that levels of AACT and LRG1 expression are increased in ovarian cancer sera, and validated this by Western blot ...five iTRAQ ® experiments with 1 or ...
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iTRAQ based proteomic analysis of adaptive response in the regenerating limb of the Cynops orientalis newt
... It was reported that proteolysis by several proteases is crucial for regulating intestinal regeneration in Holothuria glaberrima (Pasten et al., 2012). We found that three of four proteasome proteins were down-regulated ...
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Approaches for Improved Positional Proteomics
... using iTRAQ, with all sites corresponding to caspase-3 previously reported cleavage ...incorporated iTRAQ whole protein labeling with terminal amine isotopic labeling (iTRAQ-TAILS) to enrich the ...
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Comparative proteomics analysis of myocardium in mouse model of diabetic cardiomyopathy using the iTRAQ technique
... All animals were acclimated in the cages for 15 weeks be- fore being euthanized. C57BLKS/J db/db mice were used as a DM group, while db/m mice were used as a normal con- trol (NC) group. All the animals were weighed each ...
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A Peptidomic Analysis of the Potential Comorbidity Biomarkers for Type 2 Diabetes Mellitus and Alzheimer’s Disease
... spectrometry analysis and iTRAQ quantification were used for identification of peptidomic analysis, then detection of three comorbidity biomarkers might be associated with T2DM and AD by ...
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Original Article iTRAQ-based proteomics analysis of hippocampus and striatum in rats with hyperglycemia and Parkinson’s disease
... against database. For protein identification, 0.05 Da (2ppm) mass tolerance was permitted for intact peptide masses and 0.05 Da for frag- mented ions, allowing for one missed cleavage in the trypsin digests. ...
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iTRAQ based quantitative proteomics analysis of rice leaves infected by Rice stripe virus reveals several proteins involved in symptom formation
... an iTRAQ approach for a quantitative proteomics comparison of non-infected and infected rice ...bioinformatics analysis indicated that ten of these regulated proteins are involved in chlorophyll ...
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iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy
... To identify new potential markers, the function of dif- ferentially expressed proteins was analyzed through PubMed literature and String analysis. Finally, six pro- teins (gelsolin, collectin-11, PTPRJ, AKAP-7, ...
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Periostin identified as a potential biomarker of prostate cancer by iTRAQ-proteomics analysis of prostate biopsy
... the iTRAQ shot-gun proteomic approach has offered the option to study differential expression of proteins in perturbed ...far, iTRAQ has been used in studying the proteomics of the tissue and the cell line ...
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Tomato nuclear proteome reveals the involvement of specific E2 ubiquitin conjugating enzymes in fruit ripening
... Ubiquitin-mediated protein proteolysis plays an import- ant role in many basic cellular processes in plants, such as cell cycle, circadian rhythm control, hormone signal- ing, growth, development, stress response, and ...
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