Kluyveromyces lactis
Ineffective Phosphorylation of Mitogen-Activated Protein Kinase Hog1p in Response to High Osmotic Stress in the Yeast Kluyveromyces lactis
... stimulus, Kluyveromyces lactis cells respond by activating the mitogen-activated protein ki- nase (MAPK) ...K. lactis Hog1 (KlHog1) protein via two conserved branches, SLN1 and ...K. lactis ...
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Sugar metabolism, redox balance and oxidative stress response in the respiratory yeast Kluyveromyces lactis
... propose Kluyveromyces lactis as a good alternative model to analyse variants in the oxidative stress response, since the respiratory metabolism in this yeast is predominant under aerobic conditions and it ...
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The Gα Subunit Signals through the Ste50 Protein during the Mating Pheromone Response in the Yeast Kluyveromyces lactis
... Yeast mating signal transduction pathways require a heterotrimeric G protein composed of G ␣ , G  , and G ␥ subunits connected to a mitogen-activated protein kinase (MAPK) module. While in Saccharomyces cerevisiae ...
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Metabolic engineering of Kluyveromyces lactis for L-ascorbic acid (vitamin C) biosynthesis
... Kluyveromyces lactis is one of the most important non-Saccharomyces yeast species used as an eukaryotic model and tool for biotechnological applications includ- ing an alternative host for heterologous gene ...
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Transcriptomic Analysis of Extensive Changes in Metabolic Regulation in Kluyveromyces lactis Strains
... Kluyveromyces lactis is one of the major yeasts in ...K. lactis is the most studied nonconventional yeast and has become an alternative model to study yeast physiology, as its physiology is very ...
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Theme and Variation Among Silencing Proteins in Saccharomyces cerevisiae and Kluyveromyces lactis
... yeast Kluyveromyces lactis, which contains cryptic copies of the mating type ...K. lactis complements the silencing defect of sir4 null mutations in ...K. lactis sir2 and sir4 mutant strains ...
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Connection Between the Rag4 Glucose Sensor and the KlRgt1 Repressor in Kluyveromyces lactis
... cerevisiae, Kluyveromyces lactis is a predominantly aerobic yeast that exhibits a distinct regulation pattern for glucose ...K. lactis, the low-affinity glucose uptake is glucose inducible and the ...
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RAS/Cyclic AMP and Transcription Factor Msn2 Regulate Mating and Mating-Type Switching in the Yeast Kluyveromyces lactis
... as Kluyveromyces lactis, first induce mating in response to ...K. lactis, mating and mating-type switching are induced by the DNA-binding protein ...
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Use of the KlADH3 promoter for the quantitative production of the murine PDE5A isoforms in the yeast Kluyveromyces lactis
... hypertension. Kluyveromyces lactis, one of the few yeasts capable of utilizing lactose, is an attractive host alternative to Saccharomyces cerevisiae for heterologous protein ...K. lactis as a ...
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Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase
... K. lactis and ...(ECLACZ), Kluyveromyces lactis β -galactosidase (KLLAC4) and Aspergillus niger β -galacatosidase ...K. lactis and ...K. lactis β -galactosidase important for catalytic ...
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Mannosyl-Diinositolphospho-Ceramide, the Major Yeast Plasma Membrane Sphingolipid, Governs Toxicity of Kluyveromyces lactis Zymocin
... Kluyveromyces lactis zymocin, a trimeric ( ␣␥ ) protein toxin complex, inhibits proliferation of Saccharomyces cerevisiae cells. Here we present an analysis of kti6 mutants, which resist exogenous zymocin ...
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Kluyveromyces lactis Sir2p Regulates Cation Sensitivity and Maintains a Specialized Chromatin Structure at the Cryptic α-Locus
... yeast Kluyveromyces lactis. The K. lactis MAT, HMRa, and HML␣ loci shared flanking DNA sequences on both sides of the loci presumably acting as recombinational targets during mating type ...K. ...
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The Inactivation of KlNOT4, a Kluyveromyces lactis Gene Encoding a Component of the CCR4-NOT Complex, Reveals New Regulatory Functions
... yeast Kluyveromyces lactis, which encodes a component of the evolutionarily conserved CCR4-NOT ...K. lactis strains lacking Not4p are defective in fermentation and show reduced transcription of ...
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Characterization of KlGRR1 and SMS1 Genes, Two New Elements of the Glucose Signaling Pathway of Kluyveromyces lactis
... K. lactis glucose signaling pathway by comparison to the same pathway of ...K. lactis genome, we identified a single gene, SMS1 (for similar to Mth1 and Std1), that encodes a protein showing an average of ...
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Effects of physicochemical parameters on volatile sulphur compound formation from l-methionine catabolism by non-growing cells of Kluyveromyces lactis
... Kluyveromyces lactis KL71 was obtained from Danisco Singapore Pte Ltd ...K. lactis KL71 was prepared by inoculating 5% (v/v) of a pure culture in the sterilized broth (100 mL) and incubated at 30 °C ...
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RAG4 Gene Encodes a Glucose Sensor in Kluyveromyces lactis
... of Kluyveromyces lactis was previously isolated as a fermentation-deficient mutant, in which transcription of the major glucose transporter gene RAG1 was ...K. lactis appears to have no other genes ...
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Functional Analysis of the Single Est1/Ebs1 Homologue in Kluyveromyces lactis Reveals Roles in both Telomere Maintenance and Rapamycin Resistance
... Est1 and Ebs1 in Saccharomyces cerevisiae are paralogous proteins that arose through whole-genome duplication and that serve distinct functions in telomere maintenance and translational regulation. Here we present our ...
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Heterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae
... yeasts Kluyveromyces lactis GG799 (and protease deficient derivatives such as YCT390) and Saccharomyces cerevisiae YPH499 were transformed with the optimised genes and transformants were screened for ...
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Protein Kinases Involved in Mating and Osmotic Stress in the Yeast Kluyveromyces lactis
... yeast Kluyveromyces lactis, the signal transduction system that mediates the mating re- sponse is triggered by both the G ␣ ...(K. lactis Gpa1p [KlGpa1p]) (31) and G  (KlSte4p) (14) subunits of the ...
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Dealing with different methods for Kluyveromyces lactis β-galactosidase purification
... from Kluyveromyces lactis is an enzyme which has attracted our attention since it represents an essential material to convert the waste product cheese whey into a substrate valuable for biotechnology ...
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