laser scanning confocal microscope
Designing a laser scanning confocal microscope: a new scan engine layout
... Imaging has been around for ages. Since the evolution of the first light detecting cells organisms have been able to notice the world through vision. As time progressed the eye evolved, using lenses to be able to focus ...
38
A novel optical microscope for imaging large embryos and tissue volumes with sub-cellular resolution throughout
... with laser scanning confocal microscopes in the mid-1980s, it became obvious that the optical sectioning, which is the main advantage of the confocal method, did not work with the available ...
15
ZIP4, a Novel Determinant of Tumor Invasion in Hepatocellular Carcinoma, Contributes to Tumor Recurrence after Liver Transplantation
... (A) Laser-scanning confocal microscope was used to detect the distribution of ZIP4 in HepG2, BEL7402, and HL-7702 cells (magnification: ...
12
Non Contact Surface Morphology Analysis of CO2 Laser Irradiated Teeth by Scanning Electron Microscope and Confocal Laser Scanning Microscope
... the laser irradiation effect after acid etching was also ...The laser was applied perpendicularly to non-etched and 35% phosphoric acid-etched ...by laser irradiation through surface analysis in ...
8
In vivo slit scanning confocal microscopic observation in a patient with moderate and severe keratoconus: a case report
... In vivo slit scanning confocal microscopy (ConfoScan4, Nidek Technologies Srl, Albignasego, Italy), was carried out to evaluate the corneal cell morphology. The nonaplanatic water immersion 40× objective ...
5
Reduction of the pulse duration of the ultrafast laser pulses of the Two Photon Laser Scanning Microscopy (2PLSM)
... the laser cavity the over- all GVD has to be near ...s laser cavity free from the problems of cubic dispersion, asym- metric spectra and increased sensitivity of pulse width to cavity and prism ...
7
Fluorescence lifetime imaging of E combretastatin uptake and distribution in live mammalian cells
... FLIM fluorescence was isolated by combined narrowband interference (400IU25) and BG3 (Comar) filters. The sample could also be imaged using a confocal imaging system (Nikon eC1-Si) on the same microscope ...
23
Drastic effect of shearing on graphite microtexture: attention and application to Earth science
... using confocal laser scanning microscopy, laser Raman spectroscopy, and focused ion beam – transmission electron microscopy (FIB – TEM) to elucidate the effect on surface condition and ...
12
The Relationship of Surface Roughness and Work Function of Pure Silver by Numerical Modeling
... Surface of silver has important effects on its performance in natural environments. Surface roughness and work function of the silver samples were measured using confocal laser scanning microscopy ...
13
Microscanners for optical endomicroscopic applications
... the scanning laser beam is parallel to an endoscopic cam- ...dual-axis confocal (DAC) endomicroscope has also be developed for forward-looking tissue imag- ...dual-axes confocal reflectance ...
11
Characterisation of solidification using combined confocal scanning laser microscopy with infrared thermography
... Confocal scanning laser microscopy is a growing technique as it offers the unique capability to observe (amongst other things) the solidification of high melting point materials such as ...
20
Viability Gradients in Biofilm: A slippery slope?
... Confocal laser scanning microscopy (CLSM) involves taking a series of thin optical sections at different depths through a sample. This is attained by employing a pinhole aperture to block ...
27
Confusion over live/dead stainings for the detection of vital microorganisms in oral biofilms - which stain is suitable?
... In this context the FDA/EB vital fluorescence staining was routinely used together with Confocal Laser Scanning Microscopy (CLSM) [119,124,131, 134-136,139,140,143,153] to establish the [r] ...
12
The study of n-3PUFAs protecting the intestinal barrier in rat HS/R model
... The proximal jejunum specimens were harvested imme- diately after the animals were sacrificed, and rinsed in cold saline with subsequent immersion in 10% buffered formalin over- night at room temperature. Sections ...
9
Iris ultrastructure in patients with synechiae as revealed by in vivo laser scanning confocal microscopy
... the laser scanning confocal micro- scope resembles a tree-like structure that branches from the surface of the iris that contacts the cornea to the dee- per ...
6
<p>Nano-Scaled Zeolitic Imidazole Framework-8 as an Efficient Carrier for the Intracellular Delivery of RNase A in Cancer Treatment</p>
... by confocal laser scanning microscopy (CLSM), and the inhibition of cell proliferation after the delivery of RNase A was evaluated by MTT assay, Live/Dead staining, and TUNEL cell apoptosis analysis, ...
11
Analysis of plant tissue images obtained by confocal tandem scanning reflected light microscope
... Immediately after cutting, the sample was rinsed in distilled water in order to remove starch and other cell components that potentially remained on the surface. Next, the slice was mounted on a microscopy slide and ...
5
Cryo-laser scanning confocal microscopy of diffusible plant compounds
... fresh tissue infusion remains the most efficient and user- friendly method. It is well adapted to turgid plant tissue due to its porosity and exchange properties. Applied to apple and grape tissues, labelling was ...
8
Original Article Renalase overexpression in ER-positive breast cancer
... and laser confocal scanning microscope observations were used to detect expression of renalase, estrogen receptor (ER), phospho-extracellular signal-regulated kinase 1 and 2 (p-ERK1/2), and ...
11
Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal
... In order to estimate the minimum force for scratching the crystal surface, we estimated the extent of the force of the tip that affects surface morphology by using a hy- brid microscope. Figure 4 shows typical ...
5