Leishmania braziliensis
Epitope Mapping of the HSP83.1 Protein of Leishmania braziliensis Discloses Novel Targets for Immunodiagnosis of Tegumentary and Visceral Clinical Forms of Leishmaniasis
... among Leishmania species but are divergent from the host species Homo sapiens and Canis familiaris and from Trypanosoma cruzi, the parasite that causes Chagas disease, to select potential targets for the ...
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Leishmania braziliensis and Leishmania amazonensis amastigote extracts differ in their enhancement effect on Leishmania infection when injected intradermally
... of Leishmania amazonensis amastigote extract (LaE) in BALB/c mice exacerbates the infection of these mice by Leishmania ...a Leishmania braziliensis amastigote extract (LbE) would exert the ...
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Species specific ecological niche modelling predicts different range contractions for Lutzomyia intermedia and a related vector of Leishmania braziliensis following climate change in South America
... of Leishmania braziliensis, the causative agent of much cutaneous leishmaniasis (CL) in South America [7, ...L. braziliensis [4, 9] in different regions, such as ...
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Presence of Leishmania braziliensis in blood samples from cured patients or at different stages of immunotherapy.
... By using a PCR assay specific for Leishmania braziliensis in blood samples, we detected this parasite in patients cured by immunotherapy or at different stages of treatment.. We also fou[r] ...
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Host biting rate and susceptibility of some suspected vectors to Leishmania braziliensis
... the Leishmania braziliensis is concentrated in the sore; even so the infection rate was greater than that observed for experimental infection by Leishmania tropica in Phlebotornus duboscqi females ...
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In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L donovani, L major and L mexicana
... Promastigotes of Leishmania braziliensis, L. donovani, L. major and L. mexicana recently derived from tissue amastigotes were cultured in Schneider’s Drosophila medium supplemented with 20 % (v } v) ...
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Antigenic specificity of the 72 kilodalton major surface glycoprotein of Leishmania braziliensis braziliensis
... braziliensis 72-kDa major surface antigen was not recognized by sera from patients with visceral leishmaniasis or Chagas' disease, although recognition of this antigen was achieved by se[r] ...
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Identification of the HSP70 II gene in Leishmania braziliensis HSP70 locus: genomic organization and UTRs characterization
... L. braziliensis cells was isolated using the phenol-chloroform-isoamilic alcohol method ...L. braziliensis chromosomes were prepared from promastigotes, harvested during log phase, washed and embedded in ...
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Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages
... of Leishmania are consistent with the reports from many Old World Leishmania species and strains which showed that LPG with its varied structural polymorphisms in- duced different levels of NO and TNF-α in ...
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Involvement of nucleoside diphosphate kinase b and elongation factor 2 in Leishmania braziliensis antimony resistance phenotype
... Western blot assays were carried out for investigating the expression level of NDKb and EF2 proteins in the transfected parasites and in the Sb III -resistant and -sus- ceptible lines of L. braziliensis and L. ...
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Leishmania braziliensis replication protein A subunit 1: molecular modelling, protein expression and analysis of its affinity for both DNA and RNA
... L. braziliensis RPA-1 (LbRPA-1) protein based on its binding to the 5′ and 3′ untranslated regions (UTR) of the 70 kDa heat shock pro- tein (HSP70) mRNAs ...L. braziliensis RPA-1 protein conforms the typ- ...
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Production of a kinesin related recombinant protein (Lbk39) from Leishmania braziliensis by Leishmania tarentolae promastigotes and its application in the serodiagnosis of leishmaniasis
... For expression of the target recombinant protein, the synthetic gene Lbk39 was.. assembled from synthetic oligonucleotides by Invitrogen Germany, and the fragment.[r] ...
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Mannosyltransferase (GPI 14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony
... in Leishmania is through aquaglyceroporin 1 (AQP1), a six helices plasma transmembrane pore forming protein ...protects Leishmania from complement-mediated damage, inhibiting channel forma- tion and lysis ...
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An integrated pipeline for the development of novel panels of mapped microsatellite markers for Leishmania donovani complex, Leishmania braziliensis and Leishmania major.
... the Leishmania genomes that have been sequenced, it is relatively straightforward but laborious to manually extract mapped microsatellites and design primers for PCR (Jamjoom et ...L. braziliensis, ...
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An integrated pipeline for the development of novel panels of mapped microsatellite markers for Leishmania donovani complex, Leishmania braziliensis and Leishmania major.
... In addition to the development of the micro- satellite extractor programme we have also assessed the use of clinical samples and nested-PCR to avoid the need for culturing in the genotyping of Leishmania ...
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Functional analysis of cytosolic tryparedoxin peroxidase in antimony resistant and –susceptible Leishmania braziliensis and Leishmania infantum lines
... We transfected wild-type L. braziliensis and L. infantum lines with the construct pIR1-BSD-LbcTXNPx to generate transfectants overexpressing cTXNPx. Linearization of the vector allowed integration of the construct ...
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Leishmania braziliensis SCD6 and RBP42 proteins, two factors with RNA binding capacity
... To determine the ability of rLbSCD6 and rLbRBP42 pro- teins to bind RNA, pull down assays were performed, fol- lowing the protocol described by Nocua et al. [23] using as target the L. braziliensis 3′ HSP70-II UTR ...
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IL 32γ promotes the healing of murine cutaneous lesions caused by Leishmania braziliensis infection in contrast to Leishmania amazonensis
... (V.) braziliensis (MHOM/BR/2003/IMG) strains were obtained from patients with localized cuta- neous lesions [19, ...L. braziliensis strain was obtained and identified by our group, as described by Dorta et ...
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Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia
... of Leishmania is complex due to the absence of accurate population genetics tools that correlate with the biology of the protozoan ...of Leishmania is controversial, some authors have demonstrated genetic ...
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Proteins Selected in Leishmania (Viannia) braziliensis by an Immunoproteomic Approach with Potential Serodiagnosis Applications for Tegumentary Leishmaniasis
... Immunoblot assays and identification of target proteins in sta- tionary promastigote and amastigote-like forms of Leishmania braziliensis. To evaluate the antigenicity of the spots resolved in the 2DE gels ...
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