Lentiviral transduction
Amelioration of emphysema in mice through lentiviral transduction of long lived pulmonary alveolar macrophages
... of lentiviral vector carrying either a negative control (CMV-GFP) or luciferase reporter gene ...single lentiviral (CMV-luc) infection, imaged 1 week (C) and 16 months (D) later in the same ...of ...
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An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo
... lentivirus transduction in ...that lentiviral transduction of MGSCs in vivo is a simple, efficient, low labor- intensive approach to produce transgenic ...
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Characterization of Bovine Induced Pluripotent Stem Cells by Lentiviral Transduction of Reprogramming Factor Fusion Proteins
... Pluripotent stem cells from domesticated animals have potential applications in transgenic breeding. Here, we describe induced pluripotent stem (iPS) cells derived from bovine fetal fibroblasts by lentiviral ...
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In vivo selection of hematopoietic progenitor cells and temozolomide dose intensification in rhesus macaques through lentiviral transduction with a drug resistance gene
... with lentiviral vectors encoding MGMT* and a fluorescent marker, with or without homeobox B4 (HOXB4), a potent stem cell self-renewal ...confirmed transduction of long-term repopulating ...
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Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages
... used lentiviral vectors to transduce CD34+ cells with a Tar decoy alone or in combination with an anti-CCR5 ribozyme that down regulates an essential HIV-1 ...
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High level β globin expression and preferred intragenic integration after lentiviral transduction of human cord blood stem cells
... human HSCs. The products of the LM-PCR include genomic sequenc- es flanking the integrated provirus, thus allowing the site of provi- ral integration to be ascertained by DNA sequencing. Using this approach, we analyzed ...
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Alteration of T cell immunity by lentiviral transduction of human monocyte derived dendritic cells
... Polarization of naïve Th cells into Th1 cells is critical for the induction of cellular immunity against intracellular pathogens and cancer cells. The observed impairment of the Th1 response by LV-transduced DCs raises a ...
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Leukemogenic Fusion Gene (p190 BCR ABL) Transduction into Hematopoietic Stem/Progenitor Cells in the Common Marmoset
... BCR-ABL lentiviral vector (HIV-CMV/ PGK- BCR-ABL) and cultured without ...after lentiviral transduction, RT-PCR for the BCR- ABL gene was performed in BCR-ABL- or mock-trans- duced cell ...after ...
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Lentiviral vector transduction of spermatozoa as a tool for the study of early development
... Integration of viral vectors in porcine spermatozoa was con- firmed by LAM-PCR analysis. However, no integration sites in the mouse genome were detected following lentiviral transduction of murine ...
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Anti-tat Hutat2:Fc mediated protection against tat-induced neurotoxicity and HIV-1 replication in human monocyte-derived macrophages
... by lentiviral vector ...signal transduction-related genes, including Fas, P53, CASP3, and NFKB2, in 15 candidate genes were not significantly changed following transduction as com- pared to ...
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Deciphering the molecular basis of invasiveness in Sdhb deficient cells
... KRT19 lentiviral transduction induced a massive up-regulation of Krt19 mRNA level in both cell types (1,000-fold induction, Supplementary Figure 6A), immunofluorescence analysis showed no differences on ...
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Role of Phosphatidylserine Receptors in Enveloped Virus Infection
... MFG-E8 enhances transduction by lentiviral vectors. We first determined whether MFG-E8 can increase lentivirus binding and transduction by bridging virus and cells. Using HMVECs that express Axl ...
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Regulation of ICP0-Null Mutant Herpes Simplex Virus Type 1 Infection by ND10 Components ATRX and hDaxx
... Nomenclature for HepaRG-derived cell lines generated by lentiviral transduction Name Phenotype Drugs to which cells are resistant HALL HAA HALD2 HL-E HL-ED HD-E HD-ED HepaRG shLuci HepaR[r] ...
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Induction of Human Blood Group A Antigen Expression on Mouse Cells, Using Lentiviral Gene Transduction
... Because the EF-1 a promoter is not liver specific it is possi- ble that confounding factors played a role in modulating the humoral response observed in our experiments. It has been reported previously that ...
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Enhanced Central Nervous System Transduction with Lentiviral Vectors Pseudotyped with RVG/HIV-1gp41 Chimeric Envelope Glycoproteins
... HIV-1 lentiviral vector with its homologous gp41 envelope glycoprotein (GP) cytoplasmic tail (CT), we created chimeric RVG/HIV-1gp41 GPs composed of the extracellular and transmembrane sequences of RVG and either ...
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Development of a Human Immunodeficiency Virus Type 1-Based Lentiviral Vector That Allows Efficient Transduction of both Human and Rhesus Blood Cells
... inhibiting transduction, several reasons explain why the addition of SIV Vif did not further improve transduction rates in rhesus blood ...our lentiviral vectors do not contain the APOBEC3G protein ...
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Transduction with Lentiviral Vectors Altered the Expression Profile of Host MicroRNAs
... Stable transduction with shRNA vectors exhibited nonspecific off-target ef- ...deployed lentiviral shRNA vectors (obtained from the com- monly used TRC RNAi library [Sigma-Aldrich, Switzerland]) to knock ...
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Expressing exogenous functional odorant receptors in cultured olfactory sensory neurons
... Investigations of OR ligand specificity have been challeng- ing due to the difficulty of expressing ORs in heterologous cells. Amino-terminal modification by adding a rho- dopsin tag to the OR coding sequence enhanced ...
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Efficient and Highly Specific Gene Transfer Using Mutated Lentiviral Vectors Redirected with Bispecific Antibodies
... Chen and colleagues developed a redirection system based on covalent coupling of Sindbis pseudotyped lentiviruses and cell-specific adaptors that relied on the high- affinity, covalent pairing of bacterial proteins SpyTag ...
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Original Article VE-statin/Egfl7 expression in malignant glioma and its relevant molecular network
... signal transduction is a relatively definite linear cas- cade that involves the ligand TGF-β/BMP, the receptor complex TGFβR1/2, and signal trans- duction factors of the Smad ...signal transduction of ...
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