Lifetime imaging
Mapping the lignin distribution in pretreated sugarcane bagasse by confocal and fluorescence lifetime imaging microscopy
... fluorescence lifetime imaging microscopy (FLIM) are able to provide important information about the concen- tration and the spatial distribution of a fluorophore within a sample, since this molecule has a ...
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Time-domain fluorescence lifetime imaging techniques suitable for solid-state imaging sensor arrays
... high-speed lifetime imaging is lifetime ...the lifetime calculations while maintaining enough imaging ...conventional imaging systems or simply inverters and digital buffers in ...
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Signal peptide peptidase (SPP) dimer formation as assessed by fluorescence lifetime imaging microscopy (FLIM) in intact cells
... Fluorescence lifetime imaging microscopy (FLIM) can be is used to determine intra- or intermolecular interactions by fluorescently labeling epitopes on one or two different ...fluorophore lifetime ...
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Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches
... Time-correlated single photon counting (TCSPC) systems are widely as the gold standard fluorescence lifetime imaging (FLIM) owing to high temporal resolution. The acquisition speed has been significantly ...
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Using in vivo fluorescence lifetime imaging to detect HER2-positive tumors
... Fluorescence lifetime imaging is based on evaluation of the average time that electronically excited fluoro- phore stays in that excited state before its transition to a ground state, accompanied by photon ...
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Single Cell Assay for Molecular Diagnostics and Medicine: Monitoring Intracellular Concentrations of Macromolecules by Two-photon Fluorescence Lifetime Imaging
... fluorescence lifetime imaging ...fluorescence lifetime of fluorophore and the refractive index of its microen- vironment varying due to changes in the concentrations of macromolecules, mainly ...
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Fluorescence lifetime imaging of E combretastatin uptake and distribution in live mammalian cells
... fluorescence lifetime imaging was used with two-photon excitation ...fluorescence lifetime imaging with 2PE to study uptake and intracellular disposition of E-CA4 and an ...
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Towards unsupervised fluorescence lifetime imaging using low dimensional variable projection
... fluorescence lifetime imaging (FLIM) data is becoming overwhelming; the latest FLIM systems easily produce massive amounts of data, making an efficient analysis more challenging than ...
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Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
... Fluorescence lifetime, defined as how long a fluorophore remains in its excited state, is sensitive to intermolecular interactions and changes of microenvironment while it is independent of in- tensity variations ...
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Single-shot time-gated fluorescence lifetime imaging using three-frame images
... fluorescence lifetime imaging (FLI) technology with high ...fluorescence lifetime imaging using three-frame images (TFI-TGFLI), is ...rapid lifetime determination (RLD) scheme and a ...
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A fast regularized least-squares method for retinal vascular oxygen tension estimation using a phosphorescence lifetime imaging model
... The oxygen tension of retinal vessels can also be measured using a phosphorescence lifetime imaging model (PLIM) [5-7]. PLIM is a noninvasive method, though it requires intravenous injection, and does not ...
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GPU acceleration of time-domain fluorescence lifetime imaging
... Fluorescence lifetime imaging microscopy (FLIM) plays a significant role in biological sciences, chem- istry, and medical ...up lifetime calculations compared to CPU-OpenMP (parallel computing with ...
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Molecular rheometry : direct determination of viscosity in Lo and Ld lipid phases via fluorescence lifetime imaging
... required, with about 10–15% of a fast (B1 ns) component. The value or the amplitude of this component did not change with lipid composition. The more dominant (85–90%) longer lifetime compo- nent (2–3.7 ns), on ...
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Time resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A 4 in solution and in cellular systems
... fluorescence lifetime of E-DMACA4 in DMSO ...the lifetime image in CHO cells at ...fluorescence lifetime is longer than those of the other combretastatins ...the lifetime of the other ...
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Direct Vpr Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging
... Investigating intracellular Vpr-Vpr interaction by FLIM Due to its exquisite dependence on the inter-chromo- phore distance, FRET between eGFP- and mCherry tagged proteins will occur only if they are less than 10 nm ...
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Luminescence lifetime imaging microscopy by confocal pinhole shifting (LLIM-CPS)
... Imaging was performed with a Zeiss LSM 510 META confocal microscope (Carl Zeiss, GmbH) equipped with internal Argon (Ar) and Helium-Neon (He-Ne) lasers and a Coherent femtosecond pulsed Ti-Sapphire laser for ...
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A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging
... Further to imaging EGFR/Grb2 signalling dynamics in living cells MM-FLIM was appied to the analysis of ligand-dependent receptor heterodimerisation. We examined the interaction between the EGFR family members HER2 ...
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International Journal of Materials Lifetime
... Here, it has been deemed appropriate to study and analyze the performance characteristics of a magnetic fluid based rough short bearing considering the thin film lubrication at nano s[r] ...
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(Anti)hypertriton lifetime puzzle
... the lifetime of (anti)hypertriton gave a similar lifetime which is close to the lifetime of free Λ ...(anti)hypertriton lifetime demonstrate a much short ...(anti)hypertriton lifetime ...
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Comparison of effective dose and lifetime risk of cancer Incidence of CT attenuation correction acquisitions and radiopharmaceutical administration for myocardial perfusion imaging
... different to the quoted figures due to the absence of additional breast tissue that would be present in the female phantom but as a comparison within the context of this study, some interesting results were found. This ...
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