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0.01 M Tris-maleate buffer, pH 6.5 containing 0.15 M NaCl

Use of a Novel Enzyme Immunoassay Based on Detection of Circulating Antigen in Serum for Diagnosis of Helicobacter pylori Infection

Use of a Novel Enzyme Immunoassay Based on Detection of Circulating Antigen in Serum for Diagnosis of Helicobacter pylori Infection

... 0.05 M Tris buffer containing 200 mM NaCl and Tris-buffered saline [TBS; pH ...and 5 noninfected individuals, as proven by microbiological culture, were used to ...

5

Dissociation of polyoma virus by the chelation of calcium ions found associated with purified virions.

Dissociation of polyoma virus by the chelation of calcium ions found associated with purified virions.

... After adding virus to the dissociation shows the dissociated virions after a 15-min mixture 10 mM EGTA, 3 mM DTT, and 0.15 M NaCl in 0.01 M Tris buffer, pH 8.5, portions were exposure to[r] ...

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Quantitative and Qualitative Chemical Extraction of Deoxyribo Nucleic Acid DNA from Human Cell Organelles

Quantitative and Qualitative Chemical Extraction of Deoxyribo Nucleic Acid DNA from Human Cell Organelles

... The pH was adjusted ...the pH adjustment. iii. 5M NaCl: 292.2 grams NaCl was dissolved in 800 ml of distilled water and distilled water added to make final volume of 1 ...Lysis Buffer ...

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Forms of Deoxyribonucleic Acid Produced by Virions of the Ribonucleic Acid Tumor Viruses

Forms of Deoxyribonucleic Acid Produced by Virions of the Ribonucleic Acid Tumor Viruses

... Endonuclease assay Three forms of DNA might be expected to buffer contained 0.1 M Tris-hydrochloride, pH 7.6, occur in the reaction: double-stranded DNA 0.1 M NaCl, and 0.01 M magnesium [r] ...

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Internal initiation of translation on the vesicular stomatitis virus phosphoprotein mRNA yields a second protein.

Internal initiation of translation on the vesicular stomatitis virus phosphoprotein mRNA yields a second protein.

... VSV mRNA was synthesized in vitro in a reaction mixture containing final concentration Tris hydrochloride pH 8.0 50 mM, NaCI 0.1 M, dithiothreitol 4 mM, MgCl2 5 mM, ATP, CTP, and GTP 1 m[r] ...

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An efficient order reduction strategy in earthquake nonlinear response analysis of structures

An efficient order reduction strategy in earthquake nonlinear response analysis of structures

... The first paper regarding the field of structural dynamics was written by Cusumano et al. [9] in the early 1990’s, who presented an experimental study of dimensionality in an elastic impact oscillator. In the papers [10] ...

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Evidence for the Essential Arginine and Histidine Residues in Catalytic Activity of Glucose 6-Phosphate Dehydrogenase from Streptomyces aureofaciens

Evidence for the Essential Arginine and Histidine Residues in Catalytic Activity of Glucose 6-Phosphate Dehydrogenase from Streptomyces aureofaciens

... reagent, with a molar ratio to enzyme concentration of 100, about 80% of the enzyme activity was lost within 5 min, followed by a slow phase of inactivation. The slow phase could be due to a conformational change ...

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High Sensitive Voltammetric Determination of Paroxetine on Glassy Carbon Electrode Modified with Nafion/MWCNTs

High Sensitive Voltammetric Determination of Paroxetine on Glassy Carbon Electrode Modified with Nafion/MWCNTs

... rinsed and sonicated 5 min in distilled water. Next 10 mg of MWNTs was added to 10 mL ethanol and Nafion (final Nafion concentration 0.1 %), and then sonicated for 2 hours to obtain a homogenous suspension. The ...

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Volumetric properties of aqueous solutions of sodium cyclamate in presence of (0 1, 0 3, and 0 5) m fructose at different temperatures

Volumetric properties of aqueous solutions of sodium cyclamate in presence of (0 1, 0 3, and 0 5) m fructose at different temperatures

... Na-Cyclamate + water + 0.1 m fructose 0.00 1003.98 1002.53 1000.92 999.13 0.02 1005.53 1004.07 1002.44 1000.63 0.04 1007.07 1005.60 1003.95 1002.12 0.06 1008.60 1007.12 1005.45 1003.60 0.08 1010.12 1008.63 1006.94 ...

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Quantitation of glutathione S-transferases in rice (Oryza sativa L.) roots exposed to cadmium by liquid chromatography–tandem mass spectrometry using isotope-labeled wing peptides as an internal standard

Quantitation of glutathione S-transferases in rice (Oryza sativa L.) roots exposed to cadmium by liquid chromatography–tandem mass spectrometry using isotope-labeled wing peptides as an internal standard

... medium containing kanamycin at 37 °C until the cell density reached ...mM Tris–HCl (pH 8.0) and 150 mM NaCl, and then ...supernatants containing OsGSTU6 and OsGSTF14 proteins were ...

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Convention on International Trade in Endangered Species of Wild Fauna and Flora. EC annual report 2003

Convention on International Trade in Endangered Species of Wild Fauna and Flora. EC annual report 2003

... Exp/Imp. App. Annex Taxon Term Quantity Unit Importer Exporter Origin Purpose Source I 1 A Tapirus indicus live 1 ES SG B C I 1 A Rhinocerotidae spp. carvings 1 DE CH XX T O I 1 A Rhinocerotidae spp. carvings 1 FR CH KE ...

400

Studies of light neutron-rich nuclei

Studies of light neutron-rich nuclei

... (i) Light ion reactions. These were used first, because of the 3 availability of suitable beams. For example, the (t,p) and (t, He) reactions change the isospin of the target nucleus by +1 (AT = +1). The reaction “* * * ...

189

Virus-Associated Nucleases: Location and Properties of Deoxyribonucleases and Ribonucleases in Purified Frog Virus 3

Virus-Associated Nucleases: Location and Properties of Deoxyribonucleases and Ribonucleases in Purified Frog Virus 3

... Purified FV3 was assayed for deoxyribonuclease activity in the presence of NP40 as described in text except that, as indicated, the buffer used was either 0.1 M sodium acetate, pH 5.0; 0[r] ...

9

Voltammetry of Adiponectin and its Interactions with Collagen on a Carbon Paste Electrode at Femtogram Level

Voltammetry of Adiponectin and its Interactions with Collagen on a Carbon Paste Electrode at Femtogram Level

... Further, the influence of various incubation times (0, 20, 40, 60, 80, 100, 120, 140 and 160) was studied (Fig. 4C). Signal of collagen did not change and was subtracted. The measured Y signal steadily increased ...

12

Photodynamic treatment of herpes simplex virus during its replicative cycle.

Photodynamic treatment of herpes simplex virus during its replicative cycle.

... TNE buffer contained 0.01 M Trishydrochloride pH 7.4, 0.1 M NaCl, and 0.001 M 16 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Photodynamic treatment of herpes simple[r] ...

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Equilibrium Studies, DNA Binding Studies and Anti Microbial Activity of VO(II) and Zn(II) Complexes
of 2-Acetyl-amino-5-methyl-1,3,4-thiadiazole

Equilibrium Studies, DNA Binding Studies and Anti Microbial Activity of VO(II) and Zn(II) Complexes of 2-Acetyl-amino-5-methyl-1,3,4-thiadiazole

... and chemical shift in 𝛿 ppm. Thermogravimetric analyses were carried out on Perkin-Elmer model TGS-2. Conductance of the metal complexes was determined in DMSO using Digisun digital conductivity meter model D1 909. ...

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Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

... has also not been studied extensively in vivo, especially in situations of diabetes-related healing deficit [11] [12]. Characterization of an aqueous extract of human placenta which is used as a licensed drug, leads to ...

13

A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form

A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form

... PBS buffer at pH 8 containing different molar concentration of urea (0-8 M) were used to solubilize EGFP and MMP-12-CAT inclusion bodies and the solubility achieved in 2 M urea ...

12

Differentiation among closely related organisms of the Actinobacillus Haemophilus Pasteurella group by means of lysozyme and EDTA

Differentiation among closely related organisms of the Actinobacillus Haemophilus Pasteurella group by means of lysozyme and EDTA

... Bacteriolysis in Tris-maleate buffer 0.005 M, pH 7.2 supplemented with EDTA 0.01 M and hen egg white lysozyme HEWL, 1.0 ,ug/ml was set up to assist differentiation between the taxonomica[r] ...

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