Maltose-binding protein, MBP
RNA-Stimulated ATPase and RNA Helicase Activities and RNA Binding Domain of Hepatitis G Virus Nonstructural Protein 3
... nonstructural protein 3 (NS3) contains amino acid sequence motifs typical of ATPase and RNA helicase ...NS3 protein, the NS3 region (amino acids 904 to 1580) was fused with maltose-binding ...
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Sequence requirements for stable binding and function of Rep68 on the adeno-associated virus type 2 inverted terminal repeats.
... This report demonstrates the ability of both recombinant fusion maltose-binding protein MBP-Rep68A produced in Escherichia coli and wild-type wt Rep68 to bind to a linear truncated form [r] ...
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A maltose-binding protein/adeno-associated virus Rep68 fusion protein has DNA-RNA helicase and ATPase activities.
... Rep68 protein produced in Escherichia coli as a fusion protein with maltose-binding protein (MBP-Rep68 D ) has previously been shown to possess DNA-DNA helicase activity, as does ...
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Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1
... recombinant protein aggregation are not only host-specific but also target protein specific, no univer- sal remedy for inclusion body formation is ...with maltose-binding protein ...
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Construction of a strep-tag II mutant maltose binding protein for reagentless fluorescence sensing
... Maltose binding protein (MBP) changes its conformational structure upon its ligand ...make MBP an ideal candidate for reagentless fluorescence sensing. MBP gene, (malE) was ...
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Strep-tag ii mutant maltose-binding protein for reagentless fluorescence sensing
... Abstract: Maltose-binding protein (MBP) is a periplasmic binding protein found in Gram negative ...bacteria. MBP is involved in maltose transport and bacterial ...
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Late onset X linked sideroblastic anemia Missense mutations in the erythroid delta aminolevulinate synthase (ALAS2) gene in two pyridoxine responsive patients initially diagnosed with acquired refractory anemia and ringed sideroblasts
... Abbreviations used in this paper: ALAS, aminolevulinate synthase; ALAS2, erythroid-specific 6-ALAS gene; Hb, hemoglobin; MBP, maltose-binding protein; MCV, mean corpuscular volume; nt, n[r] ...
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Antigenicity of Recombinant Maltose Binding Protein-Mycobacterium avium subsp. paratuberculosis Fusion Proteins with and without Factor Xa Cleaving
... as MBP fusion proteins at the Bacterial Diseases of Livestock Research Unit, USDA-ARS Agricultural Research Service National Animal Disease Cen- ter (NADC) (Ames, IA), and one (MAP1272c) was produced as a His- ...
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Characterization of a tryptophanyl-tRNA synthetase Urzyme and its intramolecular complementation establishes a model to study the catalytic mechanism and evolution of the contemporary Class I aminoacyl-tRNA synthetases
... the maltose binding protein carrier which probably will increase the storage time of purified material in 50% glycerol at -20 o ...considering MBP a protecting cage that helps preserve the ...
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ATPase Regulation in the Maltose Transporter
... soluble protein enables it to be expressed in far greater copy ...associated binding partners in the membrane, up to 4.6x10 4 MBP molecules are typically present kjfin the periplasm of ...1000 ...
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Human eosinophil major basic protein is an endogenous allosteric antagonist at the inhibitory muscarinic M2 receptor
... basic protein (MBP) as well as other eosinophil proteins, on binding of [3H]N-methyl-scopolamine ([3H]NMS: 1 x 10(-10) M) to muscarinic M2 receptors in heart membranes and M3 receptors in ...
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Myelin associated serological targets as applicable to diagnostic tools to be used at the preclinical and transient stages of multiple sclerosis progression
... One of the most significant constituents of myelin sheath in etiopathogenesis of MS is myelin-associated oligodendrocytic basic protein (MOBP), a potential tar- get for the autoreactive T cell clones. In murine ...
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Low-dose monobutyl phthalate stimulates steroidogenesis through steroidogenic acute regulatory protein regulated by SF-1, GATA-4 and C/EBP-beta in mouse Leydig tumor cells
... 1 binding sites resulted in decreased StAR expression in in vitro reporter gene assays [24,40], and it has been reported that SF-1 knockout mice did not express StAR mRNA, indicating SF-1 is required for StAR gene ...
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A Leucine Zipper-Like Domain Is Essential for Dimerization and Encapsidation of Bluetongue Virus Nucleocapsid Protein VP4
... SDS-PAGE and Western blot analysis. Protein samples were analyzed by SDS-PAGE with either a 10 or 8% polyacrylamide resolving gel (11). The gels were either stained with Coomassie brilliant blue or electroblotted ...
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Biologically active Rep proteins of adeno-associated virus type 2 produced as fusion proteins in Escherichia coli.
... Recombinant MBP-Rep68 and MBP-Rep78 proteins displayed the following activities reported for wild-type Rep proteins when assayed in vitro: i binding to the AAV inverted terminal repeat I[r] ...
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Reactivity to myelin antigens in multiple sclerosis Peripheral blood lymphocytes respond predominantly to myelin oligodendrocyte glycoprotein
... basic protein (MBP) and proteolipid protein (PLP), are likely to be of importance in the course of multiple sclerosis (MS), cell-mediated autoimmune responses to other myelin antigens, in particular ...
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Myelin in multiple sclerosis is developmentally immature
... involving protein chemical, mass spectrometric, and electron microscopic techniques we have determined that myelin obtained from victims of MS is arrested at the level of the first growth spurt (within the first 6 ...
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Decreased dependence of myelin basic protein reactive T cells on CD28 mediated costimulation in multiple sclerosis patients A marker of activated/memory T cells
... basic protein (MBP)-specific T cells, the activation state of these cells has not been well ...of MBP-reactive T cells on CD28-mediated costimulation in MS patients, healthy controls, and stroke ...
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Design of Linear and Cyclic Mutant Analogues of Dirucotide Peptide (MBP82–98) against Multiple Sclerosis: Conformational and Binding Studies to MHC Class II
... shown to suppress autoreactive T cells following intravenous injections in preclinical and clinical studies [15]. In a phase II human clinical trial in HLA-DR2 and HLA-DR4 patients with MS, dirucotide was shown to be ...
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Impact of Human Immunodeficiency Virus Type 1 gp41 Amino Acid Substitutions Selected during Enfuvirtide Treatment on gp41 Binding and Antiviral Potency of Enfuvirtide In Vitro
... ENF binding to the mutated target and their effects on susceptibility to inhibition by ...ENF binding or susceptibility conferred by the aa substitutions studied (Spear- man nonparametric correlation ...
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