Membrane-associated proteins
The Conserved ATPase Get3/Arr4 Modulates the Activity of Membrane-Associated Proteins in Saccharomyces cerevisiae
... Sepharose. Proteins copurifying with Get3-ProA were eluted by TEV pro- tease cleavage, separated by SDS–PAGE, and detected by silver ...two proteins were also found among the proteins pu- rified with ...
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Benevides Martins, Gustavo (2019): Subcellular localization and dynamics of transmembrane and membrane associated proteins in Corynebacterium glutamicum. Dissertation, LMU München: Fakultät für Biologie
... cytoplasmic membrane of Corynebacterium glutamicum Corynebacterium glutamicum is a facultative anaerobic, gram-positive, non- motile, non-sporulating bacterium that in nature, inhabits mainly the surface layers of ...
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Structural Analysis of the Roles of Influenza A Virus Membrane-Associated Proteins in Assembly and Morphology
... the membrane of the protrusions appeared thicker than that in pleomorphic protrusions lacking ...the membrane was covered by a uniform layer of glycoproteins, both along the pro- trusions and on the ...
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Localization of Membrane-Associated Proteins in Vesicular Stomatitis Virus by Use of Hydrophobic Membrane Probes and Cross-Linking Reagents
... When any of these reagents was reacted with virus in the presence of 1 mg of cytochrome c per ml, there was no labeling of the cytochrome c data not shown, indicating that this system of[r] ...
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A New Mutant Class, Made by Targeted Mutagenesis, of Phage PRD1 Reveals That Protein P5 Connects the Receptor Binding Protein to the Vertex
... the proteins being precipitated with the viral ...of membrane-associated proteins was sur- prising, and further studies have revealed that the structural proteins can be divided into ...
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A proteomic and phosphoproteomic analysis of Oryza sativa plasma membrane and vacuolar membrane
... 230 membrane and membrane associated proteins in plasma membrane and tonoplast fractions of rice root and shoot often occurs in a tissue specific manner and that around 20% of the ...
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Artificial membranes for membrane protein purification, functionality and structure studies
... Membrane proteins account for ~30% of both prokaryotic and eukaryotic ...Integral proteins such as transporters or ion channels, as well as peripheral membrane proteins such as ...
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Vaccinia Virus L2 Protein Associates with the Endoplasmic Reticulum near the Growing Edge of Crescent Precursors of Immature Virions and Stabilizes a Subset of Viral Membrane Proteins
... two proteins needed for crescent formation: A17 (56) and A11 ...protein associated with the entry-fusion com- plex embedded in the MV ...the proteins were resolved by SDS- PAGE and analyzed by ...
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Membrane-Bound Tomato Mosaic Virus Replication Proteins Participate in RNA Synthesis and Are Associated with Host Proteins in a Pattern Distinct from Those That Are Not Membrane Bound
... replication proteins were associated with membranes ...replication proteins in the iBYL S15 fraction did not show an RdRp activity even when exogenous ToMV RNA was added ...replication ...
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Interactions of the TGB1 Protein during Cell-to-Cell Movement of Barley Stripe Mosaic Virus
... The requirements for systemic plant virus infections differ from those of animal viruses because the plant cell wall poses a barrier that must be breached to permit initial infections, cell-to-cell movement, and entry ...
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A versatile strategy for production of membrane proteins with diverse topologies: application to investigation of bacterial homologues of human divalent metal ion and nucleoside transporters
... was membrane-spanning (Fig 1B) but that it was not a signal sequence, unlike in some eukaryotic family members ...the proteins would be located on the cyto- plasmic side of the ...such proteins, one ...
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A 12-Residue-long Polyleucine Tail Is Sufficient to Anchor Synaptobrevin to the Endoplasmic Reticulum Membrane
... ER membrane and (ii) the minimum length of the hydrophobic segment required for transmembrane inte- gration, the wild-type C-terminal region of Syb2 was replaced by an idealized transmembrane segment composed of ...
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Strategies for the purification of membrane proteins
... Although membrane proteins account for 20–30% of the coding regions of all sequenced genomes and play crucial roles in many fundamental cell processes, there are relatively few membranes with known 3D ...
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Abstract: In order to alter and adjust the shape of the membrane, cells harness various mechanisms of curvature
... for membrane curvature generating ...between membrane shape, membrane curvature, and membrane proteins ...convex proteins (indicated with red cones) aggregate and flow toward the ...
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An investigation into FtsE and the 76 minute morphogene cluster in "Escherichia coli"
... the proteins in the samples were acetone precipitated, a variety o f strategies were tried to overcome ...these proteins could not be successfully manipulated at ...
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A quantitative proteomic analysis of the tegumental proteins from Schistosoma mansoni schistosomula reveals novel potential therapeutic targets
... the membrane has proven to be a valid method to locate surface exposed proteins on the api- cal membrane of adult parasites (Braschi and Wilson, 2006; Mulvenna et ...tegumental proteins from ...
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Strategies for the purification of membrane proteins
... solubilize proteins by binding to the hydrophobic parts of the protein on one side and interacting with the aqueous parts on the other side ...the membrane protein without irreversibly denaturing ...
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Lu, Aiping (2008): Development of proteomics techniques for individual protein and membrane protein mapping. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... of proteins, proteomics potentially can provide insights into the entire ...single proteins, their posttranslational modifications, and protein-protein interactions as well as on a global scale such as ...
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Adherence and invasive properties of Corynebacterium diphtheriae strains correlates with the predicted membrane-associated and secreted proteome
... secreted proteins (407/ 549 proteins) was predicted to be non-classical secreted proteins without any signal peptide, although only 49 of them are conserved in all 20 ...(1–7 proteins) are ...
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Characterization of Nonstructural Protein Membrane Anchor Deletion Mutants Expressed in the Context of the Hepatitis C Virus Polyprotein
... NS5B proteins of the ex- pected molecular masses of 21, 70, 56 to 58, and 68 kDa, respectively, could be detected in lysates of cells transfected with pUHDHCV(H)con, indicating robust expression and cor- ...of ...
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