Minor groove
Shape selective recognition of the DNA minor groove by hairpin polyamides
... of minor groove-binding polyamides as tools for genomic analysis and expansion of the minor groove recognition code by designing internal and N-terminal replacements for ...
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Sequence-selective minor groove recognition of a DNA duplex containing synthetic genetic components
... impact minor groove ...other minor groove binders such as Hoechst33258 in complex with A-tract dsDNA ...uences groove width but also reduces allosteric modulation in a DNA ...of ...
9
Synthesis of New Series of Bis Benzothiazole Derivatives as DNA Minor Groove Binding Agents
... Based on these structural studies, molecular modelling has suggested to us that compound 5 could effectively bind into the DNA minor groove (Figure 1). Then, a series of derivatives with a head-to-head ...
6
Comparative effects of A.T and G.C sequence selective DNA minor groove binding agents
... in minor groove binder-DNA interactions is the 'phasing' of the bond forming moieties of the ligand and the DNA (Lown, ...the minor groove of DNA (Goodsell & Dickerson, ...
227
Minor groove binders for DNA as antitypanosomal agents : the veterinary context
... to minor groove binding drugs from Strathclyde (S-MGBs) which is important because compounds useful in the field must have activity across the range of infectious species so that ...
19
Four pyrrole derivatives used as building blocks in the synthesis of minor groove binders
... amido)-N-[2-(morpholin-4-yl)ethyl]-1H-pyrrole-2-carboxamide}, are inter- mediates used in the synthesis of modified DNA minor-groove binders. In all four compounds, the nitro groups lie in the plane of the ...
24
Rationalising sequence selection by ligand assemblies in the DNA minor groove : the case for thiazotropsin A
... Selective recognition of short sequence nucleic acid targets or larger transcription factor binding sites by sequence reading, minor groove binding small molecules (MGBs) remains rela- tively difficult, ...
12
Four pyrrole derivatives used as building blocks in the synthesis of minor-groove binders
... amido)-N-[2-(morpholin-4-yl)ethyl]-1H-pyrrole-2-carboxamide}, are inter- mediates used in the synthesis of modified DNA minor-groove binders. In all four compounds, the nitro groups lie in the plane of the ...
25
Detection of the Bacillus anthracis gyrA Gene by Using a Minor Groove Binder Probe
... Identification of chromosomal markers for rapid detection of Bacillus anthracis is difficult because significant chromosomal homology exists among B. anthracis, Bacillus cereus, and Bacillus thuringiensis. We evaluated ...
7
Thiazotropsin aggregation and its relationship to molecular recognition in the DNA minor groove
... Nuclear Magnetic Resonance (NMR) spectroscopy and isothermal titration calorimetry (ITC) studies of DNA binding by the thiazotropsin class of molecules show no observable evidence of single molecule binding in a ...
37
Chemical and photo-induced nuclease activity of a novel minor groove DNA binder Cu(II) complex
... a groove binder molecule DAPI was studied by fluor- escence spectroscopy in order to understand whether the complex is able to displace DAPI molecules from DNA minor groove or EB molecule from DNA ...
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Impact of pyrrolidine-bispyrrole DNA minor groove binding agents and chirality on global proteomic profile in Escherichia Coli
... target minor grooves of duplex DNA in order to control specific gene expression implicated in a disease ...of minor groove bin- ders on DNA ...
9
Establishment of a minor groove binder probe based quantitative real time PCR to detect Borrelia burgdorferi sensu lato and differentiation of Borrelia spielmanii by osp A specific conventional PCR
... Background: Borrelia burgdorferi sensu lato (sl), the causative agent of Lyme borreliosis, is transmitted by ticks of the genus Ixodes as vector. For identification of Borrelia infections in ticks a TaqMan™ minor ...
10
A simplified duplex real time PCR incorporating TaqMan minor groove binder (MGB) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae cultures
... cause their small size enables binding in the minor groove of double-stranded DNA, thus stabilising the duplex, which results in high melting temperature (Tm) values (Kutyavin et al. 2000; Guo et al. 2009), ...
6
Activity of the DNA minor groove cross-linking agent SG2000 (SJG-136) against canine tumours
... SG2000 demonstrated a similar level of potent in vitro ac- tivity against a panel of canine tumour cell lines as has been previously demonstrated against human tumour cells. Cytotoxicity correlates with the formation and ...
16
Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes
... and minor groove binders, which attach between the helical ...the minor groove binders, such as SYTO-13, have been less well studied despite being potentially less perturbative to the DNA ...
29
Design, synthesis and antibacterial activity of minor groove binders: the role of non-cationic tail groups
... non-cationic minor groove binders with neutral tail groups attached to the three backbones that gave highest antibacterial activity in earlier studies ...
9
Protein and drug interactions in the minor groove of DNA
... In order to investigate whether these small differences in inter-atomic distance can explain the differences in frequencies of one-atom motifs from Table 4, energies of the averaged motifs have been compared with ...
10
An evaluation of minor groove binders as anti-lung cancer therapeutics
... major groove; however, with the minor groove binding affinity conferred by the distamycin framework, tallimustine has been shown to preferentially monoalkylate the 3’-adenine-N3 atom of 5’-TTTTGA-3’ ...
11
Ranking ligand affinity for the DNA minor groove by experiment and simulation
... DNA minor groove it has become possible to develop tailored approaches to drug ...of minor groove binders (MGBs) proceeded from the observation that netropsin and distamycin bound selectively ...
13