Molecular beacon
Quantitative Detection of the M204V Hepatitis B Virus Minor Variants by Amplification Refractory Mutation System Real Time PCR Combined with Molecular Beacon Technology
... with molecular beacon ...the molecular beacon ...with molecular beacon technology has a much higher discriminatory ability ...
7
Use of a Multiplex Molecular Beacon Platform for Rapid Detection of Methicillin and Vancomycin Resistance in Staphylococcus aureus
... and molecular beacon sequences for six unique targets are listed in Table ...BAC16S molecular beacon probe, 0.4 M of SG16S molecular beacon probe, ...mecA molecular ...
7
MicroRNA Detection Using a Double Molecular Beacon Approach: Distinguishing Between miRNA and Pre-miRNA
... double molecular beacon approach to miRNA assessment that can distinguish and quantify mature versus precursor forms of miRNA in a single assay, an essential feature for use of miRNAs as biomarkers for ...
13
The use of nanocrystal quantum dot as fluorophore reporters in molecular beacon-based assays
... The utilization of molecular beacon (MB) biosensor probes to detect nucleic acid targets has received enormous inter- est within the scientific community. This interest has been stimulated by the ...
13
Use of Sloppy Molecular Beacon Probes for Identification of Mycobacterial Species
... ular beacon probes in PCR assays generates hybrids that are sometimes relatively weak because they possess many mis- matched base ...sloppy molecular beacon probe A and seven different myco- ...
9
Molecular beacon probes-base multiplex NASBA Real-time for detection of HIV-1 and HCV.
... Primers and probes design. We designed two sets of primers and molecular beacon probes on pol gene and 5’NCR region of HIV-1 and HCV viruses, respectively. All the conserved sequences were selected by ...
8
Simultaneous Detection of Pathogens in Clinical Samples from Patients with Community Acquired Pneumonia by Real Time PCR with Pathogen Specific Molecular Beacon Probes
... of molecular probes of original design on demand and fluorescent oligonucleotides in clinical labora- ...a molecular beacon (MB) probe (15, 31) and a TaqMan probe (2, 8, 9, 33) have allowed more ...
7
Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis Isolates from India and Mexico by a Molecular Beacon Assay
... a molecular beacon-based real- time PCR assay for this purpose (14, 15) and later converted this method into a multicolor, single-tube assay format ...single-well molecular beacon assay used ...
5
Development of real time NASBA assays with molecular beacon detection to quantify mRNA coding for HHV 8 lytic and latent genes
... A method for the detection of RNA is RT-PCR. To increase sensitivity an additional amplification step can follow the RT-PCR. This method is time consuming and also increas- es the likelihood of false positive reactions ...
10
APPLICATION OF MOLECULAR BEACON BASED BIOSENSOR AGAINST rs699 SNP IN HYPERTENSIVE AND NON- HYPERTENSIVE PUNJABI POPULATION
... the molecular beacon assays on a solid surface, the specific/nonspecific signal ratios obtained are in the range of only 2 - 6 46 - 48 ...immobilized molecular beacons can distinguish a G/C mismatch ...
14
Rapid Identification of Candida dubliniensis Using a Species Specific Molecular Beacon
... develop molecular beacon probes for rapid, high-fidelity identification of ...the molecular beacons were independently verified by random amplified polymorphic DNA analysis-based geno- typing and by ...
8
Development of a Molecular Beacon Assay To Detect the G1896A Precore Mutation in Hepatitis B Virus Infected Individuals
... The molecular-beacon assay is inexpensive, rapid, specific, and sim- ple compared to other assays used to detect the 1896 PC mutation, such as direct sequencing, primer-extension assays, allele-specific ...
5
Genotypic Susceptibility Testing of Mycobacterium tuberculosis Isolates for Amikacin and Kanamycin Resistance by Use of a Rapid Sloppy Molecular Beacon Based Assay Identifies More Cases of Low Level Drug Resistance than Phenotypic Lowenstein Jensen Testing
... Resistance to amikacin (AMK) and kanamycin (KAN) in clinical Mycobacterium tuberculosis strains is largely determined by specific mutations in the rrs gene and eis gene promoter. We developed a rapid, multiplexed sloppy ...
9
New Panfungal Real Time PCR Assay for Diagnosis of Invasive Fungal Infections
... and others (7/60). An overall sensitivity of 83.3% was achieved, showing similar sensitivity values in patients with proven and probable IFI. We were able to provide identification to the species level for 77.8% of those ...
9
Multiplex Real Time PCR for Rapid Staphylococcal Cassette Chromosome mec Typing
... all molecular beacon targets using conserved regions, the universality of any given target cannot be guaranteed, due to the heterogeneity of SCCmec ...hybrid beacon is used to detect both ccrB1 and ...
15
Nucleic Acid Sequence Based Amplification Assays for Rapid Detection of West Nile and St Louis Encephalitis Viruses
... Manual. Molecular beacon probes for the NASBA assays were de- signed with the help of the NucliSens Basic Kit World Wide Web-hosted help ...virus molecular beacon ...
8
Development of a Nucleic Acid Sequence Based Amplification Assay That Uses gag Based Molecular Beacons To Distinguish between Human Immunodeficiency Virus Type 1 Subtype C and C′ Infections in Ethiopia
... the molecular beacons for the present study, we utilized the principle of incorporating additional mismatches as a way to enhance the specificity, as described earlier ...The molecular beacons were targeted ...
8
Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells
... siRNA molecular beacon ...employing molecular beacon siRNA technology one can start to assess the target RNA engagement – a stage between initial cellular internalization and final gene ...
9
Comparison of RNA and DNA-Based Amplification Methods for the Discrimination of Viable from Non-Viable Salmonella Typhimurium
... Treatment 1, Pure Culture: Thirty-five ml of TSB-A was inoculated with 10 µl of an overnight culture of S. Typhimurium and grown at 37 ° C for 10 h. After incubation, cells were washed twice to remove residual DNases and ...
94
Ultrasensitive Amplification Refractory Mutation System Real Time PCR (ARMS RT PCR) Assay for Detection of Minority Hepatitis B Virus Resistant Strains in the Era of Personalized Medicine
... with molecular beacon assay quantitatively detected, apart from the dominant clones, minor variant popula- tions, even when they represented ...with molecular beacon assay is more sensitive in ...
8