Mre11-Rad50-Xrs2 complex
Mutations in Mre11 Phosphoesterase Motif I That Impair Saccharomyces cerevisiae Mre11-Rad50-Xrs2 Complex Stability in Addition to Nuclease Activity
... with mre11-D16A in high copy was not restored to the length observed in the mre11-D56A, suggesting that other functions at the telomere might be impaired by the mre11-D16A ...showed ...
10
Multiple Endonucleases Function to Repair Covalent Topoisomerase I Complexes in Saccharomyces cerevisiae
... and Mre11 participate in repairing campto- thecin-induced damage: Sae2 was originally identified by its requirement in meiotic recombination, a require- ment that can be bypassed by a mutation in SPO11 (McKee and ...
10
The Saccharomyces cerevisiae mre11(ts) Allele Confers a Separation of DNA Repair and Telomere Maintenance Functions
... yeast Mre11 protein participates in important cellular functions such as DNA repair and telomere ...of Mre11 has led to identification of several separation-of-function mutations as well as N- and ...
8
The Drosophila Nbs Protein Functions in Multiple Pathways for the Maintenance of Genome Stability
... and rad50 nbs double mutants are significantly higher than those observed in the corresponding single mutants (Table ...ATM/ Rad50/Mre11 ...homolog), Rad50, Mre11, and Xrs2 (the ...
8
Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe
... MRN complex is called Mre11- Rad50-Xrs2 (MRX), NHEJ requires MRX in an end-joining assay that monitors recircularization of restriction enzyme- cut plasmids transformed into yeast (Zhang and ...
16
Differential Suppression of DNA Repair Deficiencies of Yeast rad50, mre11 and xrs2 Mutants by EXO1 and TLC1 (the RNA Component of Telomerase)
... in rad50 strains, but was reduced ⵑ50- fold in exo1 rad50 double mutants (similar to Rec ⫺ rad51 cells), demonstrating that basal levels of Exo1 can substi- tute for the ...
14
The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldarius
... conserved Rad50 and Mre11 proteins must play important roles in these processes even if their precise function is still ...Eucarya, Rad50 and Mre11 proteins are associated with a third ...
10
The Mre11p/Rad50p/Xrs2p Complex and the Tel1p Function in a Single Pathway for Telomere Maintenance in Yeast
... MRX complex could affect accessi- bility of the telomere to cellular exonucleases as well as ...by mre11, rad50, and xrs2 strains, lack of phosphorylation by Tel1p does not affect all of the ...
5
Role of the Nuclease Activity of Saccharomyces cerevisiae Mre11 in Repair of DNA Double-Strand Breaks in Mitotic Cells
... RMX complex has ssDNA endonuclease and 3⬘-to-5⬘ dsDNA exo- nuclease activities, as well as a weak DNA helicase activ- ...the Mre11 subunit forms specific associ- ations with DNA, Rad50, Xrs2, ...
14
Alteration of N-Terminal Phosphoesterase Signature Motifs Inactivates Saccharomyces cerevisiae Mre11
... cerevisiae Mre11, Rad50, and Xrs2 function in a protein complex that is important for nonhomologous ...of MRE11, RAD50, and XRS2 are characterized by ionizing radiation ...
10
Ataxia-telangiectasia Mutated Kinase (ATM) as a Central Regulator of Radiation-induced DNA Damage Response
... G1/S checkpoint is controlled by interaction of E2F pro- tein (a member of essential transcription factors family) with pRb (retinoblastoma susceptibility protein). E2F is associated with DNA in the promoter region; ...
5
A Novel Recombination Pathway Initiated by the Mre11/Rad50/Nbs1 Complex Eliminates Palindromes During Meiosis in Schizosaccharomyces pombe
... tative complex has properties similar to those of the originated through a pathway that did not involve reso- Rad1/Rad10 complex (Carr et al. 1994). lution of a H J by the Mus81/Eme1 complex. Hence, ...
14
Post-transcriptional regulation of MRE11 expression in muscle-invasive bladder tumours.
... tumour MRE11 expression is predictive of poor response to radiotherapy but not cystectomy was recently independently ...low MRE11 expression seen in poorly-responding patients. MRE11 RNA and protein ...
11
Coordination and Processing of DNA Ends During Double-Strand Break Repair: The Role of the Bacteriophage T4 Mre11/Rad50 (MR) Complex
... MR complex, which is necessary for DNA end processing in vivo but dispensable for strand exchange in vitro (in reactions that are initiated with a single-stranded ...MR complex consists of gp47 (the ...
17
Relocalization of the Mre11-Rad50-Nbs1 Complex by the Adenovirus E4 ORF3 Protein Is Required for Viral Replication
... against Mre11 (B), Rad50 (F), Nbs1 (J), and PML (N) (MRNP) and against E4 ORF3 (C, G, K, and ...O). Mre11, Rad50, Nbs1, and PML were visualized with FITC-labeled secondary antibody, and E4 ORF ...
9
DNA damage induces nucleoid compaction via the Mre11 Rad50 complex in the archaeon Haloferax volcanii
... the Mre11-Rad50 complex in nucleoid reorganization, co-ordination of DNA repair pathways and rapid cell recov- ery after DNA damage suggests that all these process are linked in ...
12
Ataxia Telangiectasia-Mutated Damage-Signaling Kinase- and Proteasome-Dependent Destruction of Mre11-Rad50-Nbs1 Subunits in Simian Virus 40-Infected Primate Cells
... toskeleton buffer containing 0.5% Triton X-100 and protease inhibitors (0.15 M aprotinin, 1 mM phenylmethylsulfonyl fluoride, and 1 M leupeptin) in an ice bath. The cells were then washed with PBS, fixed in 4% ...
13
Mimivirus reveals Mre11/Rad50 fusion proteins with a sporadic distribution in eukaryotes, bacteria, viruses and plasmids
... these Mre11/ Rad50-like fusion proteins as well as in their subsequent propagation into different cellular ...of Mre11/Rad50 homologs from viruses (such as gp46/gp47 of T4 and other marine ...
10
Adenovirus Regulates Sumoylation of Mre11-Rad50-Nbs1 Components through a Paralog-Specific Mechanism
... of Mre11 and Nbs1 ...of Mre11 and Nbs1. These results do not reveal if Mre11 and Nbs1 sumoylation is the result or the cause of MRN relocalization into E4-ORF3-con- FIG 1 Ad infection induces ...
10
Serotype-Specific Reorganization of the Mre11 Complex by Adenoviral E4orf3 Proteins
... cellular Mre11 complex and is prevented by both the E4orf3 and E4orf6 ...The Mre11/Rad50/Nbs1 proteins are targeted for proteasome-mediated degradation by the Ad5 viral E1b55K/E4orf6 ...of ...
10