MTT Method
IMMUNOLOGICAL ACTIVITY AND CRYSTALLOGRAPHICAL STUDIES OF THE CARBAZOLE DERIVATIVE FROM ANGOPHORA COSTATA
... The present study involved the isolation of natural product that has been identified as carbazole derivative, reported first time from Angophora costata of South Australian origin. Carbazole derivatives have been of ...
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Antiproliferation and cell apoptosis inducing bioactivities of constituents from Dysosma versipellis in PC3 and Bcap-37 cell lines
... Dysosma versipellis, an endangered and endemic Bereridaceae plant species of China, has been proved to be an important Chinese herbal medicine because of its biological activity. In this study, fifteen compounds were ...
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Comparison of a photometric method with standardized methods of antifungal susceptibility testing of yeasts
... (iii) MTT method. After the MICs were visually determined on each of the microtiter plates, the plates were used for MTT testing. Twenty-five microliters of RPMI 1640 medium containing 5 mg of ...
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Role of Dendrtic Cells in IgA Nephropathy Pathogenesis
... attack. Method Stimulating factors such as recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF), recombinant human interleukin-4 (rhIL-4) and tumor necrosis factor-a (TNF-a) ...the ...
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Design, Synthesis and Biological Evaluation of Novel Osthole Derivatives as Potential Agents for the Treatment of Cancer
... activity. Method: After the demethylation of osthole, the target derivatives 4a-l were successfully synthesized through two steps in high ...using MTT method, and the most potent compound was ...
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<p>Synthesis of Novel Xanthone Analogues and Their Growth Inhibitory Activity Against Human Lung Cancer A549 Cells</p>
... (MTT) method to measure the cytotoxic activity of the target compounds against human lung cancer A549 ...later, MTT (20 μ L, 5 mg/mL) was added to the cells, and the cells were incubated at 37°C for ...
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Determination of MICs for Mycobacterium avium M intracellulare complex in liquid medium by a colorimetric method
... the MTT test to assess mycobacterial growth, as demonstrated in this study, enabled us to determine the MICs of drugs against MAC (Tables 1, 2, and ...the MTT reduction test. The MTT test’s ...
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Vol 68, No 3 (2016)
... NO is released from activated macrophages and functions as a marker for inflammatory progression and cytotoxic activity. Even though NO itself possess bactericidal activity, as a result of its coupling with the ...
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Antiviral activity of garlic extract on Influenza virus
... Inhibition of influenza virus adsorption to MDCK cells and chicken red blood cells by the Garlic extract was estimated by MTT and HA methods. Its effect on the viral genome replication was determined by RT-PCR ...
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<p>Novel HDAC/Tubulin Dual Inhibitor: Design, Synthesis and Docking Studies of α-Phthalimido-Chalcone Hybrids as Potential Anticancer Agents with Apoptosis-Inducing Activity</p>
... A novel series of α -phthalimido-substituted chalcones-based hybrids as dual HDAC/tubulin inhibitors was synthesized and evaluated for its in vitro anticancer activity by MTT method agai[r] ...
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Dihydroflavonols from the leaves of Macaranga recurvata and their cytotoxic and antioxidant activities
... MTT assay: The in vitro cytotoxic activity was determined by the MTT method [15,16,17]. The breast cancer cells (MCF7, and MCF7/TAMR) were grown in RPMI 1640 medium containing 10% fetal bovine serum, ...
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Synthesis of maleimide derivatives via CuAAC click chemistry and biological evaluation of their antitumor activity against cancer cell lines
... Biological Activity Compounds 4 and 6a-g were evaluated for their cytotoxicity in vitro against hela cell line by using the MTT method [24] with doxorubicin as the positive control.. The[r] ...
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12) Gingerly effervescent tablets: investigating the effect of cytotoxicity on gingival fibroblasts and antimicrobial properties under laboratory conditions
... by MTT method, after 24, 48 and 72 hours, and then analyzing the obtained data, the results showed that the cell cytotoxicity rate of the ginger tablets is different in the passing of time, although the ...
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Cooperation of IRAK1/4 inhibitor and ABT-737 in nanoparticles for synergistic therapy of T cell acute lymphoblastic leukemia
... In vitro release of either IRAK1/4 inhibitor or ABT- 737 from optimized IRAK/ABT-NP was carried out with a dialysis method. Before the experiment, dialysis membranes (molecular weight cut-off 10 kDa) were immersed ...
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Comparative Study on Janus Kinase Enzyme Activity of Pomegranate Leaf Extract and its Active Component Ellagic Acid for Asthma
... Ellagic acid (HPLC grade) purchased from Sigma Aldrich, JNK activity assay kit, Kinase star purchased from RayBiotech, Inc., RAW 264.7 cells was purchased from National Centre for Cell Sciences (NCCS), Pune, India and ...
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SYNTHESIS, CHARACTERISATION AND BIOLOGICAL SCREENING OF Cu (II) COMPLEXES OF PINCER LIGANDS
... antibacterial activity against Candida albicans and Staphylococcus aureus (gram positive), Escherichia coli (gram negative) respectively, by using modified Kirby- Bauer disc diffusion method. The screening results ...
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Papain-mediated reduction of MTT is an artifact but not an indicator of cell apoptosis in Saccharomyces cerevisiae
... the MTT interaction in the presence and absence of papain without the cells ...the MTT was dissociated in the presence of papain and not because of the cellular ...the MTT did not move inside the ...
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GC–MS ANALYSIS AND IN VITRO CYTOTOXIC ACTIVITY OF METHANOLIC EXTRACT OF ANTIGONON LEPTOPUS FLOWERS
... Determination of cell viability by MTT Assay: The monolayer cell culture was trypsinized and the cell count was adjusted to 1.0 x 10 5 cells/ml using DMEM containing 10% FBS. To each well of the 96 well microtitre ...
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CYTOTOXIC AND ANTHELMINTIC ACTIVITIES OF METHANOLIC EXTRACT OF PARTHENIUM HYSTEROPHORUS L.
... The present study has been designed to evaluate the cytotoxic and anthelmintic activities of crude methanolic extract of whole plant of Parthenium hysterophorus L. The in-vitro cytotoxic activity of methanolic extract ...
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SYNTHESIS AND EVALUATION OF SOME NEW QUINAZOLINONES FOR THEIR PHARMACOLOGICAL ACTIVITIES
... The synthesized compounds (5a-l) were assayed for antibacterial activity against Gram-positive bacteria Bacillus subtilis, Staphylococcus aureus and Gram-negative bacteria Escherichia coli, Klebsiella pneumonia. The ...
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