Multi-Locus Sequence Typing analysis of E. coli
Multi locus sequence typing of Escherichia coli isolates with acquired ampC genes and ampC promoter mutations
... England. Multi-locus sequence typing (MLST) was used to determine the genetic relatedness of ...E. coli strains with ampC promoter mutations, with a view to identifying the ...
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Multi locus sequence typing of oenological Saccharomyces cerevisiae strains
... In this work we used MLST analysis for molecular typing of several 89.. cerevisiae wine yeast strains, including isolates from sherry and sparkling wines, 90.[r] ...
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Multi Locus Sequence Typing scheme for Acidithiobacillus caldus strain evaluation and differentiation
... 3. Results 3.1. Comparative genomics-based MLST marker selection pipeline implementation To facilitate and improve the MLST marker selection process, a bioinformatic pipeline was implemented that iden- tifies single copy ...
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A Fast, Accurate, and Automated Workflow for Multi Locus Sequence Typing of Bacterial Isolates
... Data analysis in the current MLST workflow requires 4–5 hours of post-analysis data retrieval and processing of each ...imal sequence checking, the ST of each sample can be obtained in five minutes ...
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Comparison of classical multi locus sequence typing software for next generation sequencing data
... (8) and scalability to large numbers of isolates. The interfaces to the software applications fall into two cate- gories, those that operate on the command line and those that have a graphical interface. Command line ...
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Analysis of Escherichia coli O157 clinical isolates by multilocus sequence typing
... stx-negative E. coli O157 isolates has clin- ical significance and is important from a diagnostic stand- point because in such cases, stx- and Stx-independent procedures are required to detect strains that ...
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Analysis of a Uropathogenic Escherichia coli Clonal Group by Multilocus Sequence Typing
... comparison and, therefore, does not suffer from the drawbacks of gel-based fingerprinting methods. Sequence data are easily comparable and transferable between laboratories and are highly reproducible (2, 12). ...
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Multi Virulence Locus Sequence Typing of Staphylococcus lugdunensis Generates Results Consistent with a Clonal Population Structure and Is Reliable for Epidemiological Typing
... by multi-virulence-locus sequence typing (MVLST), targeting seven known or putative virulence-associated loci (atlL R2 , atlL R3 , hlb, isdJ, SLUG_09050, SLUG_16930, and ...multilocus ...
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Evaluating the impacts of stressors of Pseudomonas syringae pathovar tomato on the effectiveness of multi-locus variable number tandem repeat analysis and multi-locus sequence typing in microbial forensic investigations
... Our MLST results correspond with the previous find- ings that the core genome of P. syringae is highly clonal and displays very little genetic heterogeneity [18]. Each passage consisted of taking 0.1 ml of culture into ...
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Evaluation of Polymorphic Locus Sequence Typing for Candida glabrata Epidemiology
... other typing methods and strain sets (14, 24, 32, 34, ...phylogenetic analysis. Specifically, CgMT- J/M typing yielded 32 total alleles and a diversity index of ...
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Multi-locus sequence typing (MLST) of C. jejuni infections in the United States Patrick Kwan, PhD
... – Similar to the Dutch model, compares the number of human cases caused by different subtypes with their prevalence in each source, but with additional weighting explicitly added for e[r] ...
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Comparison of Probe Hybridization Array Typing to Multilocus Sequence Typing for Pathogenic Escherichia coli
... cluster analysis, only two vali- dation isolates were typed as CG58 by ...commensal E. coli isolates, but isolates included in the final validation were mostly commensal ...
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Sequence Diversity of the Escherichia coli H7 fliC Genes: Implication for a DNA Based Typing Scheme for E coli O157:H7
... comprehensive analysis that we have undertaken, which involved more fliC genes and all H type strains, has shown that sequences previously thought to be specific for H7 do not have the desired specificity and has ...
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Exploration of genetic diversity of Bacillus spp. from industrial shrimp ponds in Vietnam by multi-locus sequence typing
... Taxonomic analysis by 16S rRNA sequencing does not always allow species-level identification of Bacillus ...by multi-locus sequence typing ...22 sequence types were identified ...
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Genotyping of amino acid-producing Corynebacterium glutamicum strains based on multi-locus sequence typing (MLST) scheme
... Several strains previously regarded as Brevibacterium and Corynebacterium species had been classified to C. glutamicum [3,4]. Our analysis extends the conclusion and proposes that C. glutamicum should also contain ...
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Sequence Typing and Comparison of Population Biology of Campylobacter coli and Campylobacter jejuni
... C. coli MLST primer design. C. coli sequences that corresponded to the seven loci used in a previously described ...the sequence of tkt A5 is 5 ⬘ -TTTAAGTGCTGATATGGTGC-3 ⬘ ...each locus to be ...
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Iranian clonal population of Salmonella enterica serovar Enteritidis, char- acterized by multi-locus sequence typing (MLST) method
... Seven house keeping genes represent only a fraction of the bacteria whole genome in MLST scheme which hardly exceed 0.2% of it, but have proved a stagger- ing level of diversity for numerous bacteria (34), to let it ...
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Multi-locus sequence typing of Salmonella enterica subsp. enterica serovar Enteritidis strains in Japan between 1973 and 2004
... supernatant was discarded and the pellet was resuspended in 200 μ l of 0.1 × TE buffer before being boiled for 10 min and centrifuged at 12,000 rpm for 10 min. The superna- tant was used as a template for the primary ...
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Multi locus sequence typing of Ehrlichia ruminantium strains from geographically diverse origins and collected in Amblyomma variegatum from Uganda
... Conclusions We investigated a recently developed MLST scheme that allows direct genotyping of E. ruminantium by using glo- bal strains from diverse origins and field samples from heartwater-endemic areas in ...
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Multi-locus sequence type analysis of Shigellas pp. isolates from Tehran, Iran
... five sequence types with ST 152 as the dom- inant sequence type being composed of ...among sequence types for better prevention, control and treatment of ...
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