Multilineage differentiation
Equine Bone Marrow Derived Mesenchymal Stem Cells: Isolation and Multilineage Differentiation
... To isolate mononuclear cell fraction (MNCF), each BM aspirate was diluted with equal volumes of Dulbecco modified Eagle medium (DMEM, Gibco,UK). Atotal of 30ml diluted aspirate was loaded on 9ml of ficollhypaque ...
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Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation
... subsequent multilineage differentiation into representa- tives of the three germ layers, namely cardiomyocytes, hepato- cyte-like cells, and neural ...
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Insulin acts as a myogenic differentiation signal for neural stem cells with multilineage differentiation potential
... the differentiation potential of MLNSC may be restricted in the central nervous system, as secondary and tertiary stem cells undergo a restriction in their differentiation potential during expansion (Table ...
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Multilineage Differentiation Activity by the Human Umbilical Vein-Derived Mesenchymal Stem Cells
... Recently, instead of using the cord blood, Romanov et al. [3] and Covas et al. [13] obtained MSC starting from cells detached from the UC vein, in a manner similar to that initiating human umbilical vein endothelial cell ...
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Role of Wnt canonical pathway in hematological malignancies
... blocks multilineage differentiation; including B cell differentiation at early stages, suggesting the importance of fine tuning of Wnt/b-catenin signaling pathway for normal B cell development and ...
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Current developments in 3D bioprinting for tissue engineering
... Das S, Pati F, Choi Y-J, Rijal G, Shim J-H, Kim SW, Ray AR, Cho D-W, Ghosh S: Bioprintable, cell-laden silk fibroin–gelatin hydrogel supporting multilineage differentiation of stem cells[r] ...
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Generation of mesenchymal stromal cells from cord blood: evaluation of in vitro quality parameters prior to clinical use
... Background: Increasing evidence suggests the safety and efficacy of mesenchymal stromal cells (MSC) as advanced therapy medicinal products because of their immunomodulatory properties and supportive role in ...
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The spatiotemporal development of adipose tissue
... measuring multilineage differentiation potential, we characterized the SVF cells derived from primitive EAT as containing a population of predetermined progenitor cells that meet the criteria of MSCs, ...
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Fibroblasts derived from human embryonic stem cells direct development and repair of 3D human skin equivalents
... underwent multilineage differentiation in monolayer culture, but were unable to support epithelial tissue development and repair and produced significantly lower levels of ...
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Clinicohematological and cytogenetic profile of myelodysplastic syndromes in Pakistan-compare and contrast
... with multilineage dysplasia (RCMD) was the most common encountered WHO sub category of MDS followed by refractory anemia with excess blast (RAEB) as observed in previous study from Pakistan [3] and the least ...
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CD38 MOLECULE-A MULTILINEAGE GLYCOPROTEIN AND ITS UNIQUE EXPRESSION ON PLASMA CELLS
... KDa multilineage glycoprotein clustered as CD38 antigen which is transiently expressed on early lymphocyte precursors and more consistently on plasma cells is worth ...
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Etiology and Clinicopathological Profile of Patients with Pancytopenia
... AIDS was diagnosed in 25.1% of the study cases in patients with multilineage blood cytopenia and is now the commonest clinical condition associated with it in a central referral hospital[r] ...
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pom_14_inppt_07_CF_GE.pptx
... Choosing a Differentiation and Positioning Strategy Product differentiation Service differentiation Channel differentiation People differentiation Image differentiation.. Differentiatio[r] ...
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Biocompatibility of bone graft substitutes: effects on survival and proliferation of porcine multilineage stem cells in vitro
... Multilineage stem cells were harvested from the ili- ac bone marrow of an adult (23 months old) Göttin- gen minipig (Ellegaard Göttingen Minipigs A/S, Dal- mose, Denmark), as previously described [20]. Briefly, ...
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Umbilical cord blood expansion with nicotinamide provides long term multilineage engraftment
... We tested the hypothesis that a UCB-derived cell product consisting of stem cells expanded for 21 days in the presence of nicotinamide and a noncultured T cell fraction NiCord can accele[r] ...
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Myelodysplastic Syndrome (MDS) in a Child who Transformed to Acute Myeloid Leukaemia with Myelodysplasia-Related Changes (AML-MRC)
... and multilineage dysplasia of more than 50% with a history of previous MDS, which supports the direction of Myelodysplastic Syndrome (MDS) that transformed to acute myeloid leukemia with myel- odysplasia-related ...
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NRF2 mediated Notch pathway activation enhances hematopoietic reconstitution following myelosuppressive radiation
... medium (IMDM) with 2% FBS. Before staining, BM rbc were lysed using ACK Lysing Buffer (Quality Biological Inc.). For detection of LSK cells, whole BM cells were incubated with biotin-conju- gated monoclonal antibodies ...
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Long term repopulating ability of xenogeneic transplanted human fetal liver hematopoietic stem cells in sheep
... Abstract We previously reported on the successful engraftment and long-term multilineage expression erythroid, myeloid, lymphoid of human fetal liver hematopoietic stem cells in sheep af[r] ...
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Definitive hematopoiesis initiates through a committed erythromyeloid progenitor in the zebrafish embryo
... because differentiation is not observed in this region (Godin et ...the differentiation of embryonic HSCs into multiple definitive lineages occurs only after the seeding of other tissues, such as the fetal ...
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Multilineage hematopoietic progenitor activity generated autonomously in the mouse yolk sac: analysis using angiogenesis defective embryos
... of multilineage hematopoietic cells in the YS; whether these cells migrate to the liver and eventually contribute to adult hematopoiesis is not known (as for intraembryonic ...
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