N-5 acetylneuraminic acid
Reaction mechanism of N-acetylneuraminic acid lyase revealed by a combination of crystallography, QM/MM simulation, and mutagenesis.
... In all four subunits of the crystal structure, the carboxylate moiety of the substrate forms the same hydrogen bonding pattern with the backbone of Thr48 and Ser47 and with the hydroxyl group of Thr48 as found in the ...
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Initial Interaction of Rotavirus Strains with N-Acetylneuraminic (Sialic) Acid Residues on the Cell Surface Correlates with VP4 Genotype, Not Species of Origin
... Although our results indicate that the SA dependence and SA independence phenotypes are segregated according to the VP4 P genotype, Méndez et al. (27) reported that SA residue binding may be affected by altered VP4-VP7 ...
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High fat diet-induced inflammation and oxidative stress are attenuated by N-acetylneuraminic acid in rats
... Approval for the use of animals for the present study was given by the Animal Care and Use Committee (ACUC) of the Faculty of Medicine and Health Sci- ences, Universiti Putra Malaysia (Serdang, Selangor, MY), with ...
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Binding to Gangliosides Containing N-Acetylneuraminic Acid Is Sufficient To Mediate the Immunomodulatory Properties of the Nontoxic Mucosal Adjuvant LT-IIb(T13I)
... Prior investigations have demonstrated that the functional ligands for LT-IIa and LT-IIb are one or more types of gan- gliosides (5, 17, 38, 39). Data obtained from various investiga- tions have provided strong ...
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Molecular characterization of cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) associated with the erythrocyte antigens in dogs
... Several non-synonymous SNPs were reported in the Neu5Ac expressing cats (Types B and AB) [28–31]. In ca- nine CMAH, we detected a non-synonymous, c.554A > G (p.Lys185Arg), mutation in exon 5. The allele G or GG ...
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Nontypeable Haemophilus influenzae Has Evolved Preferential Use of N Acetylneuraminic Acid as a Host Adaptation
... H aemophilus influenzae is a host-adapted human pathogen that is categorized into typeable strains that express a polysaccharide capsule (serotypes a to f) and nontypeable (noncapsulated) H. influenzae (NTHi) strains (1). ...
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Fixation of the Human-Specific CMP-N-Acetylneuraminic Acid Hydroxylase Pseudogene and Implications of Haplotype Diversity for Human Evolution
... if such an ancient MRCA results from the hitchhiking effect of neighboring polymorphic loci under balancing selection. Indeed, CMAH is located in a telomeric re- gion 5 Mb apart from the highly polymorphic human ...
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Exogenous supplement of N-acetylneuraminic acid improves macrophage reverse cholesterol transport in apolipoprotein E-deficient mice
... Fifteen apoE −/− mice with a C57BL/6 genetic back- ground (male, 20 ± 2 g body weight) were purchased from Beijing HFK Bioscience Co., Ltd. (license number: SCXK2014–0010). All-experiments were approved by the Laboratory ...
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Edible Bird’s Nest, an Asian Health Food Supplement, Possesses Skin Lightening Activities: Identification of N Acetylneuraminic Acid as Active Ingredient
... For LC-MS/MS analysis, EBN samples were ground into powder (approximately 1 - 3 mm) and mixed tho- roughly. Ten mg of each was weighed and extracted by 1 mL of fresh Milli-Q water under sonication for 10 min. Followed by ...
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N Acetylneuraminic Acid (NANA) Stimulates in situ cyclic AMP Production in Tentacles of Sea Anemone (Aiptasia pallida): Possible Role in Chemosensitization of Nematocyst Discharge
... Measuring adhesive force and counting discharged nematocysts quantifies the in situ cnidocyte response to combined chemical and mechanical stimulation. These measurements in response to NANA sensitization were determined ...
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Structural and functional characterization of the <em>Helicobacter pylori </em>cytidine 5'-monophosphate-pseudaminic acid synthase PseF: molecular insight into substrate recognition and catalysis mechanism
... pseudaminic acid biosynthesis protein F; NmCNS, Neisseria meningitidis CMP-5-N-acetylneuraminic acid synthetase; MmCNS, Mus musculus CMP-5-N-acetylneuraminic ...
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Plasmodium gallinaceum ookinetes adhere specifically to the midgut epithelium of Aedes aegypti by interaction with a carbohydrate ligand
... by N-acetylneuraminic acid suggest that ookinetes may bind to a sialic-acid-like carbohydrate on the midgut ...sialic acid assay was used to analyze the contents of acid ...
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Cytidine monophosphate <em>N</em>-acetylneuraminic acid synthetase enhances invasion of human triple-negative breast cancer cells
... Cambridge, MA, USA) was added directly to the medium and cells were incubated for an additional ~ 2 hours. Cells were washed twice with PBS, trypsinized with Tryple Express (Invitrogen, Carlsbad, CA, USA), and counted ...
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Combination of aspirin with essential fatty acids is superior to aspirin alone to prevent or ameliorate sepsis or ARDS
... encodes 5-lipoxygenase (5-LOX), and enhanced concentrations of IL-1β in peritoneal macrophages and serum, while treatment with insulin reduced LTB4 ...lacking 5-LOX or the receptor for LTB4 produced ...
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Long-Term Evolution of the Hypervariable Region of Hepatitis C Virus in a Common-Source-Infected Cohort
... amino acid replacement are observed among epide- miologically unrelated sequences of subtype 1b or of more distantly related virus genotypes ...amino acid substitutions in the HVR while at the major- ity of ...
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Crystal structure of N (2 hydroxyethyl) 5 nitroisophthalamic acid monohydrate
... Monomethyl 5-nitrobenzene-1,3-dihydrogencarboxylate (900 mg, 4 mmol) was dissolved in methanol (5 ml), then ethanolamine (610 mg, 10 mmol) was added and the mixture was refluxed for 16 ...hydrochloric ...
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SIALIC ACID TRANSPORT IN E. COLI: ROLE OF OUTER MEMBRANE PORIN NANC
... use N-acetylneuraminic acid (Neu5Ac) as its sole carbon source even if the general outer membrane proteins OmpF and OmpC are not expressed: NanC - a monomeric outer membrane channel - allows Neu5Ac ...
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Polyunsaturated fatty acids in various macroalgal species from north Atlantic and tropical seas
... Our observations substantiate the conclusion that an appropriate choice of macroalgal species, given their high PUFA content and low n-6: n-3 ratio, may form a promising strategy to enhance food quality, ...
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Peroxyacid Oxidations: A Kinetic and Mechanistic Study of Oxidation of 3-Aminopyridine by Peroxomonosulfuric Acid
... peroxomonosulfuric acid (PMSA) have been investigated in aqueous medium in the pH range 0 – 13 at ...peroxy acid and in substrate concentration at constant ...
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5 Fluorosalicylic acid
... hydrogen-bonded chains in the packing. This feature is absent in (I). 5-Bromosalicylic acid (Liu et al., 2004) also shows a deviation between the plane of the phenyl ring and the carboxyl group. The bromo ...
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