Na+/H+ antiporter
Isolation and Semi Quantitative PCR of Na+/H+ Antiporter (SOS1 and NHX) Genes under Salinity Stress in Kochia scoparia
... plants. Na + /H + antiporters (NHX1 and SOS1) maintain the appropriate concentration of ions in the cytosol, thereby minimizing ...cytosolic Na + concentration by pumping it in the vacuole [5], ...
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Role of the Na+/H+ antiporter in rat proximal tubule bicarbonate absorption
... the antiporter in vivo as in brush border membrane ...the Na+-dependent, amiloride-sensitive Na+H+ ...is Na+-independent and ...
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Improved salt tolerance in canola (Brasica napus) plants by overexpression of Arabidopsis Na+/H+ antiporter gene AtNHX1
... limit Na+ uptake or its compartmentalization into ...vacuolar Na + /H + antiporters appear to hold great promise in improving agricultural productivity under salt and drought ...vacuolar Na + ...
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Kinetic properties of the Na+/H+ antiporter of lymphocytes from the spontaneously hypertensive rat: role of intracellular pH
... the Na+/H+ antiporter is increasingly reported as a feature of cells from hypertensive subjects but the intracellular pH (ipH) dependency of its activity has not been ...the Na+/H+ ...
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Basolateral membrane H/OH/HCO3 transport in the rat cortical thick ascending limb Evidence for an electrogenic Na/HCO3 cotransporter in parallel with a Na/H antiporter
... basolateral H/OH/HCO3 transport in the in vitro microperfused rat cortical thick ascending limb were examined by the microfluorometric determination of cell pH using ...a Na/(HCO3)n greater than 1 ...
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Apical Na+/H+ antiporter and glycolysis dependent H+ ATPase regulate intracellular pH in the rabbit S3 proximal tubule
... recovery rate by 73%, 55%, 77%, and 86%, respectively, whereas 1 mM KCN (lumen, bath) did not inhibit pHi recovery. Reduction of intracellular, but not extracellular chloride, also decreased the Na+-independent ...
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Expression of rat renal Na/H antiporter mRNA levels in response to respiratory and metabolic acidosis
... luminal Na/H ...expression, Na/H antiporter mRNA levels were measured by Northern analysis in renal cortex of rats with metabolic (6 mmol/kg body wt NH4Cl for 2 or 5 d) and respiratory ...
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Cyclic adenosine monophosphate acutely inhibits and chronically stimulates Na/H antiporter in OKP cells
... induced Na/H antiporter ...U, Na/H antiporter stimulation could be dissociated from cell ...inhibits Na/H antiporter activity, it chronically increases ...
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Effect of adaptogenic preparations on Na(+)/H(+)-antiporter function in plasma membrane of corn root cells under salinity conditions
... ried out on corn seedling roots of Zea mays L. ex- posed at 0.1 M NaCl. Before we have established that Methyure used by seed pretreating possesses a high salt protective ability as against Ivine. It was found that ...
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Ontogeny of Na/H antiporter activity in rabbit renal brush border membrane vesicles
... the Na/H antiporter was studied in renal brush border membrane vesicles (BBMV) from fetal and adult rabbits using isotopic and fluorescent ...the antiporter studied by 22Na+ uptake revealed ...
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Differential regulation of Na/H antiporter by acid in renal epithelial cells and fibroblasts
... the Na/H antiporters were different in these two cell lines (MCT IC50 = 65 nM; OKP IC50 = ...cells, Na/H antiporter mRNA abundance measured by RNA blots increased by two- to fivefold ...
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Na/H antiporter mRNA expression in single nephron segments of rat kidney cortex
... basolateral Na/H antiport activity could be involved in transcellular HCO3- secretion as well as cell volume and pH ...one Na/H antiporter, we studied the tissue distribution of ...
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Chronic metabolic acidosis causes an adaptation in the apical membrane Na/H antiporter and basolateral membrane Na(HCO3)3 symporter in the rat proximal convoluted tubule
... changed from 25 to 10 mM in the presence or absence of chloride, or peritubular [Na] was changed from 147 to 50 mM, respectively. dpHi/dt was 57% faster in tubules from acidotic animals when luminal [Na] ...
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Molecular physiology of the Na+/H+ antiporter in Escherichia coli
... 1987; Karpel et al. 1988) and nhaB (Pinner et al. 1992). The respective genes have been cloned and mapped (Fig. 2) and their nucleotide sequences determined. The deduced amino acid sequences suggest that both proteins ...
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Long term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells
... in Na/H antiporter activity that is independent of transcription and translation, whereas long-term activation of protein kinase C causes a persistent increase in antiporter activity that is ...
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A mechanism for branchial acid excretion in marine fish: identification of multiple Na+/H+ antiporter (NHE) isoforms in gills of two seawater teleosts
... Analysis of sequences from the RT-PCR products revealed the possibility of two distinct NHE isoforms in the sculpin and one in the mummichog. To our knowledge, this is the first description of an NHE-2-like isoform in ...
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Molecular cloning, phylogeny and localization of AgNHA1: the first Na+/H+ antiporter (NHA) from a metazoan, Anopheles gambiae
... (pseudo-blue; DRAQ-5) in isolated tissues of fourth instar An. gambiae larvae. (A) A composite whole mount, showing AgNHA1 expressed in the gastric caeca (GC), anterior midgut (AMG), selected cells of the posterior ...
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Abnormal Na+/H+ antiporter phenotype and turnover of immortalized lymphoblasts from type 1 diabetic patients with nephropathy
... Cellular Na+/H+ exchanger (NHE) activity is elevated in type 1 diabetic patients with nephropathy and patients with essential ...internal H+ binding was lowered in cells from patients with diabetic ...
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Na+/H+ antiporter, V H+ ATPase and Na+/K+ ATPase immunolocalization in a marine teleost (Myoxocephalus octodecemspinosus)
... of Na + /H + -exchanger 2 protein, sometimes located near the apical surface, in the sculpin ...as Na + /K + -ATPase. We also detected V-H + -ATPase staining, predominantly in cells at the ...
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Characterization of a basolateral electroneutral Na+/H+ antiporter in Atlantic lobster (Homarus americanus) hepatopancreatic epithelial vesicles
... successful clone, referred to as NHE1 (Sardet et al. 1989), is a ubiquitous housekeeping protein found within the basolateral membrane of nearly every polarized epithelial cell examined (Clark and Limbird, 1991). It is ...
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