Na+/H+ exchanger 1
The human Na+/H+ exchanger 1 is a membrane scaffold protein for extracellular signal-regulated kinase 2
... The plasma membrane Na + /H + exchanger 1 (NHE1, SLC9A1) is a major regulator of pH and volume in es- sentially all cells studied. Furthermore, NHE1 is involved in the regulation of cell ...
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Effect of osmotic shrinkage and hormones on the expression of Na+/H+ exchanger 1, Na+/K+/2Cl– cotransporter and Na+/K+ ATPase in gill pavement cells of freshwater adapted Japanese eel, Anguilla japonica
... Gill epithelial culture has been established in freshwater trout for the study of unidirectional ion fluxes and for the characterization of ion transporter properties (Kelly et al., 2000; Kelly and Wood, 2001c; Kelly and ...
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Elevated Na/H exchanger 1 (SLC9A1) emerges as a marker for tumorigenesis and prognosis in gliomas
... 16]. We also reported that NHE1 protein plays a critical role in proliferation and invasion of cultured human pri- mary glioma cells [17]. Most importantly, our recent study shows that temozolomide (TMZ) treatment ...
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Treatment of peripheral diabetic neuropathy in Zucker diabetic fatty (ZDF) rats with cariporide
... of Na+/H+-ex- changer-1 was increased in dorsal root ganglion neurons from ZDF rats and that subsequent cariporide treatment for 4 weeks improved neural defects of diabetic neuro- pathy including ...
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Analysis of Plasmodium falciparum Na+/H+ exchanger (pfnhe1) polymorphisms among imported African malaria parasites isolated in Wuhan, Central China
... DNA was amplified by nested PCR (Bio-Rad Mini MJ thermal cycler). The primary primers including NHE-A and NHE-B, and the secondary primers containing NHE-C and NHE-D were described in a previous study [29 – 31]. No ...
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In vitro susceptibility to quinine and microsatellite variations of the Plasmodium falciparum Na+/H+ exchanger (Pfnhe 1) gene: the absence of association in clinical isolates from the Republic of Congo
... Quinine (QN) remains a first-line drug for the treatment of severe malaria that is still used as a second-line ther- apy for uncomplicated malaria in many countries [1]. Despite the efficacy of QN against ...
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Glucocorticoids enhance acid activation of the Na+/H+ exchanger 3 (NHE3)
... antiporter activity had been achieved. Thus, in vivo stud- ies have suggested an important role for glucocorticoids in mediating the effect of acidosis on the antiporter, but the exact role played remains unclear from ...
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Identification of the human NHE 1 form of Na(+) H+ exchanger in rabbit renal brush border membranes
... that Na(+)-H+ exchange activity is higher significantly in fraction B than fraction ...BBM Na(+)-H+ exchange activity, did not result in a change in expression of the 97-kD protein in either ...
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NHEVNAT: an H+ V ATPase electrically coupled to a Na+:nutrient amino acid transporter (NAT) forms an Na+/H+ exchanger (NHE)
... coli Na + /H + antiporter 1) is the most well-known cation exchanger; its crystal structure and mechanism of action have been explored in a series of brilliant studies (Hunte et ...
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Structure function of recombinant Na/H exchanger regulatory factor (NHE RF)
... of Na/H exchange ...BBM Na/H ...on Na/H exchange transport involves a reversible phosphorylation reaction as well as the presence NHE-RF (4, 8, ...BBM Na/H exchange ...
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Activation of protein kinase A acutely inhibits and phosphorylates Na/H exchanger NHE 3
... exchanger is likely encoded by NHE-3. The present studies examined regulation of NHE-3 by PKA. NHE-3 was stably expressed in Na/H exchanger-deficient fibroblasts (AP-1/NHE-3 cells). PKA ...
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Na+/H+exchanger regulatory factor 1 inhibits platelet derived growth factor signaling in breast cancer cells
... (NHERF1) (also known as EBP-50 or NHERF) is a candidate tumor suppressor gene in human breast cancer. Human NHERF1 cDNA encodes a protein of 358 amino acids in length. NHERF1 and its close homolog NHERF2 (also known as ...
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A vacuolar type H+ ATPase and a Na+/H+ exchanger contribute to intracellular pH regulation in cockroach salivary ducts
... of H + secretion (Schwartz and Al-Awqati, 1985; Chambrey et ...stimulated H + secretion via V-ATPase in kidney proximal tubule cells occurs by a protein kinase A-independent mechanism but that protein ...
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Restoration of Na+/H+ exchanger NHE3 containing macrocomplexes ameliorates diabetes associated fluid loss
... Gastrointestinal complications, including gastroparesis, diar- rhea, constipation, and fecal inconsistence, are common to patients with diabetes mellitus (DM). Diabetic diarrhea attains clinical significance because of ...
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The cellular distribution of Na /H exchanger regulatory factor 1 is determined by the PDZ-I domain and regulates the malignant progression of breast cancer
... For immunofluorescence staining, the TRITC- conjugated secondary antibody (Invitrogen, Paisley, Scotland, UK) was subsequently added to the slides, and the slides were incubated on a shaker platform in the dark for ...
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Basolateral Na+/HCO3– cotransport activity is regulated by the dissociable Na+/H+ exchanger regulatory factor
... Immunoblot analysis confirmed the presence of endogenous NHE-RF in BLM preparations of normal rabbit kidney. As shown in Figure 1, polyclonal NHE- RF antibody reproducibly hybridized to a single polypeptide of ...
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Suppression of breast cancer cell growth by Na+/H+ exchanger regulatory factor 1 (NHERF1)
... G 1 - to-S progression. The accelerated G 1 -to-S progression as a result of NHERF1 knockdown is accompanied by elevated Rb phosphorylation and cyclin E expression (Figure ...
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NHE3 regulatory complexes
... (Na/H exchanger) gene family (SLC9 family) consists of nine isoforms which fall into two major subdivisions (Brett et ...(NHEs 1, 2, 3, 4 and 5) and those that are present primarily on ...
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Compensatory regulation of Na+ absorption by Na+/H+ exchanger and Na+-Cl- cotransporter in zebrafish (Danio rerio)
... of Na + content suggests that stress-induced NCC cell differenti- ation is associated with functional regulation of Na + up- ...file 1: Figure S1), while Gcm2 may negatively regulate this ...
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NaCl uptake by the branchial epithelium in freshwater teleost fish: an immunological approach to ion transport protein localization
... An antigen retrieval technique was employed to enhance the immunoreactivity of tissue sections with the various antisera used (see below) (Brown et al., 1996). This technique exposes epitopes masked during tissue ...
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