Nested Polymerase Chain Reaction (PCR)
Identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification
... through nested polymerase chain reaction ( PCR ) on the amelogenin ( AMEL ) sex marker, to calculate the specificity and sensitivity, and to compare with previous relevant ...
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The use of direct immunofluorescence and nested polymerase chain reaction in diagnosing perinatal infections of Chlamydia trachomatis
... ed polymerase chain reaction technique (nested-PCR) – PCR-Chlamydia trachomatis test (DNA Gdańsk, Poland) in order to detect chlamydia ...
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Nested polymerase chain reaction technique for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases.
... the sham group was administered with an equivalent volume of normal saline. Gpc3 and Afp mRNA was detected using nested PCR. Analysis was performed to determine statistical significance. Compared with the sham ...
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A simple and rapid method for detection of Trypanosoma evansi in the dromedary camel using a nested polymerase chain reaction
... the nested 270-bp PCR products were not amplified even from concentrations of 10 ng of DNA from other blood parasite including Thieleria annulata, Babesia bigemina and blood cells from non infected mice and cam- ...
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Comparison of the diagnostic performance of microscopic examination with nested polymerase chain reaction for optimum malaria diagnosis in Upper Myanmar
... PCR reaction, 2 μl of purified genomic DNA (approximately 10 ng of DNA) were used in a 30 μl ...the nested PCR, 1 μl of the primary PCR reactant were used as a template in a 30 μl ...
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Unusual fatal avian polyomavirus infection in nestling cockatiels (Nymphicus hollandicus) detected by nested polymerase chain reaction
... The presence of APV was proved by the nested PCR in the young cockatiel raised by the adult pair in the third nesting cycle. The PCR product am- plified from its blood sample was detected in the second ...
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Development of nested polymerase chain reaction detection of Mycosphaerella spp and its application to the study of leaf disease in Eucalyptus plantations
... that nested PCR is a suitable tech- nique for differentiating Mycosphaerella ...The nested PCR is a reliable and cost-effective method, useful for studies on the developmental ecology and epidemiology of ...
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Real-time and nested polymerase chain reaction in the diagnosis of multifocal serpiginoid choroiditis caused by Mycobacterium tuberculosis - a case report
... However, the use of real-time PCR (RT-PCR) and nested PCR (N-PCR) in MSC has not been reported. This paper aims to highlight the usefulness of PCR in identifying MTb as a causative agent for MSC leading to its ...
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A Nested Allele-Specific Multiplex Polymerase Chain Reaction Method for the Detection of DRD2 Polymorphisms
... The nested multiplex allele-specific PCR in this study offers a simple, inexpensive, and specific method that does not require additional equipment or additional ...
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Polymerase Chain Reaction Ppt Presentation
... or polymerase chain reaction is slidesfinder account? They are hybridized and polymerase ppt presentation on all of these can only available for optimizing pcr shares the purpose of ...
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The polymerase chain reaction in the charaterisation and diagnosis of lymphomas
... sizes. U sing th is approach, m onoclonality w as detected in 19 of 23^^^ an d 8 of 10^^® B-cell m alignancies. The sensitivity of detection of a m onoclonal population m ixed w ith polyclonal lym phoid cells w as 2 to ...
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Investigation of Bacterial Profile Present in Primary and Secondary Endodontic Infections by Nested and Multiplex Polymerase Chain Reaction - An Invivo study
... J.F. Siqueira et al (2005) 38 investigated the prevalence of several uncultivated oral phylotypes, as well as newly named species in primary and persistent endodontic infections associated with chronic periradicular ...
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Detection and identification of azithromycin resistance mutations on Treponema pallidum 23S rRNA gene by nested multiplex polymerase chain reaction
... pallidum. We recommend that it is important to perform the specificity test against larger representative microorganisms in the future. From the sensitivity test, we found that the detection limit of DNA of the assay was ...
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Evaluation of Polymerase Chain Reaction for Diagnosis of “Tuberculous Pleurisy”
... a nested PCR reaction was performed (22). A PCR reaction solution was prepared by mixing 15ul of a previously mixed reaction mixture (1 × PCR buffer, ...DNA polymerase enzyme and 10µl ...
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THE advent of the polymerase chain reaction in the
... Ruane and Austin first identified 21 snakes from museum collections (primarily the California Academy of Sciences and the Museum of Comparative Zoology at Harvard University), representi[r] ...
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Polymerase chain reaction: trenches to benches
... The application of molecular diagnostic techniques to the diagnosis of infectious disease is based on the identification of unique "signature sequences" in the DNA or RNA of a pa[r] ...
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Polymerase chain reaction on a viral nanoparticle
... 3 An alternative approach to the construction of materials from biological components has been to exploit an existing fibrous chassis that has been evolved within nature, which concentrates on functionalization of a ...
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Detection of leptospires in urine by polymerase chain reaction
... Under experimental conditions an amplification was obtained with DNA of Leptospira interrogans of some serovars belonging to serogroup sejroe.. However, very little or no amplification w[r] ...
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Polymerase chain reaction for detection of Mycobacterium tuberculosis
... The sensitivity of the amplification performed with target DNAs extracted by using the lysis procedure described above was estimated by using serial dilutions of a culture containing app[r] ...
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