Nicotiana benthamiana
Recombinant expression of beak and feather disease virus capsid protein and assembly of virus like particles in Nicotiana benthamiana
... N. benthamiana using an Agrobacterium-mediated system and plant expression vectors that included a bean yellow dwarf virus (BeYDV)-based replicating DNA ...
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NS3 Protein from Rice stripe virus affects the expression of endogenous genes in Nicotiana benthamiana
... RNA silencing is a defense system that interacts with viral genomic RNAs in plants. VSRs from different viral families do not share any obvious sequence similarity, but they generally bind to double stranded RNAs ...
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Influence of retinoblastoma related gene silencing on the initiation of DNA replication by African cassava mosaic virus Rep in cells of mature leaves in Nicotiana benthamiana plants
... a Nicotiana benthamiana pOri2 line, which is transformed with a transgene consisting of a direct repeat of the African cassava mosaic virus (ACMV)-replication origin (Ori) flanking a non-viral DNA region, ...
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Suppression of Bamboo Mosaic Virus Accumulation by a Putative Methyltransferase in Nicotiana benthamiana
... complete life cycle of the viruses. In regard to the replication of positive-strand RNA viruses, cellular factors of the host RNA- processing and translational machinery are often recruited for use in the viral ...
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Agrobacterium tumefaciens - mediated transformation of nicotiana benthamiana with dehalogenase gene resistant to monochloroacetic acid
... study, Nicotiana benthamiana was used as model plant in this study because this plant can genetically transform and regenerate with good efficiency (Martin et ...
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Tissue Specific Expression of a Terpene Synthase in Nicotiana benthamiana Leaves
... To study transient tissue specific linalool emission and to examine its fate, plastid targeted dual linalool/nerolidol synthase (FaNES1) was introduced into Nicotiana benthamiana leaves under LTP1, SUC2, ...
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Involvement of RDR6 in short range intercellular RNA silencing in Nicotiana benthamiana
... In plants, non-cell autonomous RNA silencing spreads between cells and over long distances. Recent work has revealed insight on the genetic and molecular components essential for cell-to-cell movement of RNA silencing in ...
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High-level diterpene production by transient expression in Nicotiana benthamiana
... Nicotiana benthamiana, which is now widely used for transient expression assays in plants, is an alternative host which may circumvent the need for optimization required with microbial ...N. ...
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Within leaf variation is the largest source of variation in agroinfiltration of Nicotiana benthamiana
... of Nicotiana benthamiana have proven to be rewarding targets for ...N. benthamiana for their characterization [22–24] or allowing their function to take place in the plant cells leading to changes in ...
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Protein Body Biogenesis and Utility in Recombinant Protein Production in Nicotiana benthamiana
... of Nicotiana benthamiana has become one of the most popular platforms for recombinant protein production and offers several advantages including flexibility and rapid scalability for protein production ...
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A Multifunctional Protein Encoded by Turkey Herpesvirus Suppresses RNA Silencing in Nicotiana benthamiana
... Many plant and animal viruses counteract RNA silencing-mediated defense by encoding diverse RNA silencing suppressors. We characterized HVT063, a multifunctional protein encoded by turkey herpesvirus (HVT), as a ...
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Functional Aspects of Silencing and Transient Expression of psbS in Nicotiana benthamiana
... as nonphotochemical quenching (NPQ) [8]. Mutation of psbS in Arabidopsis thaliana does not impair growth nor dramatically alter Photosystem I/II (PSI/PSII) processes other than NPQ [8-12]. We sought to determine if a ...
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The Regulation of Nicotiana benthamiana Gene Expression at the Early Stages of Red Clover Necrotic Mosaic Virus Infection.
... the Nicotiana benthamiana genome and its estimated size at 3 Gb, sequencing of the expressed portion provides an alternative approach to rapidly study gene ...N. benthamiana expressed sequence tags ...
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A gene expression microarray for Nicotiana benthamiana based on de novo transcriptome sequence assembly
... Background: Nicotiana benthamiana has been widely used in laboratories around the world for studying plant‑pathogen interactions and posttranscriptional gene expression ...N. benthamiana ...
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Engineering and expression of a human rotavirus candidate vaccine in Nicotiana benthamiana
... in Nicotiana benthamiana by transient expression, as plants allow rapid production of antigens at lower costs, without the risk of contamination by animal ...
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In Situ Characterization of Cymbidium Ringspot Tombusvirus Infection-Induced Posttranscriptional Gene Silencing in Nicotiana benthamiana
... In plants, posttranscriptional gene silencing (PTGS) is an ancient and effective defense mechanism against viral infection. A number of viruses encode proteins that suppress virus-activated PTGS. The p19 protein of ...
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A common set of developmental miRNAs are upregulated in Nicotiana benthamiana by diverse begomoviruses
... N. benthamiana plants were inoculated with begomoviruses and their miRNA profiles were analysed by northern blotting using specific miRNA ...N. benthamiana by TYLCV, CLCuMV/CLCuMB and CbLCuV infection with ...
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Engineer complete resistance to Cotton Leaf Curl Multan virus by the CRISPR/Cas9 system in Nicotiana benthamiana
... N. benthamiana plant leaves were co-infiltrated with two Agrobacterium strains with one harboring a binary vector expressing the Cas9 gene by 35S promoter and gRNA-1 by U6 promoter and the other harboring the ...
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Characterization of cDNAs from Nicotiana benthamiana that encode proteins which interact with tomato golden mosaic virus AL2 protein in the yeast two-hybrid system
... The macrocarrier was placed into a holder. 12.5 µl of the DNA-coated gold particle ethanol suspension were pipetted in a spiral onto the center of the installed macrocarrier, and allowed to dry completely. A stopping ...
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RDR6 Has a Broad-Spectrum but Temperature-Dependent Antiviral Defense Role in Nicotiana benthamiana
... N. benthamiana homolog of RDR6 and investi- gated the role of NbRDR6 in plant antiviral ...N. benthamiana line greatly diminished in NbRDR6 expression was used to test three RNA viruses belonging to ...
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