nonhomologous end-joining
A CRISPR Assisted Nonhomologous End Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis
... Clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated protein (Cas) systems, including CRISPR–CRISPR-associated protein 9 (Cas9) and CRISPR- Cas12a (Cpf1), have been widely used as ...
14
The akuBKU80 Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus
... homologous end joining, involving direct ligation of the strand ends independently of DNA homology ...use nonhomologous end joining. The nonhomologous end-joining ...
5
Efficient Gene Replacements in Toxoplasma gondii Strains Deficient for Nonhomologous End Joining
... of nonhomologous recombination has hampered gene targeting approaches in the model apicomplexan parasite Toxoplasma ...the nonhomologous end-joining (NHEJ) DNA repair pathway could be ...
10
Overcoming obstacles to nonhomologous end joining repair of chromosome double strand breaks
... that nonhomologous end joining ligates the proper DNA ends together (signal end to signal end and coding end to coding ...coding end present a more significant obstacle. ...
112
Mitochondrial Genome Maintenance: Roles for Nuclear Nonhomologous End-Joining Proteins in Saccharomyces cerevisiae
... Directly repeated sequences are hotspots for spontaneous deletion, in which one repeat and the interrepeat sequence are lost. In the nucleus, multiple pathways are implicated in the spontaneous generation of these ...
14
Adeno-Associated Virus Site-Specific Integration Is Mediated by Proteins of the Nonhomologous End-Joining Pathway
... nisms involved in site-specific integration, many aspects remain to be elucidated. In particular, when during the cell cycle site-specific integration occurs is unknown, and the cellular proteins which are involved have ...
10
Frameshift Mutagenesis: The Roles of Primer–Template Misalignment and the Nonhomologous End-Joining Pathway in Saccharomyces cerevisiae
... the nonhomologous end-joining ...that nonhomologous end joining is uniquely required for the de novo creation of tandem duplications from noniterated ...
10
Positive selection on the nonhomologous end-joining factor Cernunnos-XLF in the human lineage
... gous end-joining contribute to our brain size? It seems natural to assume that brain expansion should reflect an increased number of cells, and thus cell divisions during brain neurogenesis ...and ...
12
CRISPR/Cas9-Induced Double-Strand Break Repair in Arabidopsis Nonhomologous End-Joining Mutants
... ABSTRACT Double-strand breaks (DSBs) are one of the most harmful DNA lesions. Cells utilize two main pathways for DSB repair: homologous recombination (HR) and nonhomologous end-joining (NHEJ). NHEJ ...
10
<p>Nonhomologous end joining key factor XLF enhances both 5-florouracil and oxaliplatin resistance in colorectal cancer</p>
... Materials and methods: Cell viability is analyzed by sulforhodamine B staining assay. The nonhomologous end joining (NHEJ) repair ability of each cell line was determined by NHEJ reporter assay. mRNA ...
10
Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe
... microhomology-mediated end-joining, its role in nonhomologous end-joining (NHEJ) is unclear as Saccharomyces cerevisiae , Schizosacchar- omyces pombe , and mammals have different ...
16
A DNA PKcs mutation in a radiosensitive T–B– SCID patient inhibits Artemis activation and nonhomologous end joining
... the nonhomologous end-joining (NHEJ) DNA repair pathway, which results in failure of functional V(D)J ...DNA end-binding capacity of DNA-PKcs itself; rather, the presence of long P-nucleotide ...
9
Injection of an SV40 transcriptional terminator causes embryonic lethality: a possible zebrafish model for screening nonhomologous end-joining inhibitors
... Introduction: DNA repair by the nonhomologous end joining (NHEJ) pathway promotes tumor recurrence after chemotherapy and radiotherapy. Discovery of rapid and high-throughput techniques to screen for ...
9
Repair of Endonuclease-Induced Double-Strand Breaks in Saccharomyces cerevisiae: Essential Role for Genes Associated with Nonhomologous End-Joining
... by nonhomologous end-joining (NHEJ) is not well characterized in the yeast Saccharomyces ...DSB end-joining and some forms of recombination, resulted in G2 arrest and rapid cell ...
18
The Human T-Cell Leukemia Virus Type 1 Basic Leucine Zipper Factor Attenuates Repair of Double-Stranded DNA Breaks via Nonhomologous End Joining
... ABSTRACT Adult T-cell leukemia (ATL) is a fatal malignancy of CD4 ⫹ T cells in- fected with human T-cell leukemia virus type 1 (HTLV-1). ATL cells often exhibit ran- dom gross chromosomal rearrangements that are ...
19
Growth Defect and Mutator Phenotypes of RecQ-Deficient Neurospora crassa Mutants Separately Result From Homologous Recombination and Nonhomologous End Joining During Repair of DNA Double-Strand Breaks
... RecQ helicases function in the maintenance of genome stability in many organisms. The filamentous fungus Neurospora crassa has two RecQ homologs, QDE3 and RECQ2. We found that the qde-3 recQ2 double mutant showed a ...
13
CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome
... remodeler cause immunodeficiency, centromeric instability, and facial anomalies (ICF) syndrome types 3 and 4. Here, we demonstrate that the classical nonhomologous end joining (C-NHEJ) proteins Ku80 ...
16
Ku DNA End-Binding Activity Promotes Repair Fidelity and Influences End-Processing During Nonhomologous End-Joining in Saccharomyces cerevisiae
... in nonhomologous end-joining (NHEJ) of DNA double-strand breaks ...DNA end-binding ...microhomology-mediated end- joining (MMEJ) and were neither promoted by Mre11 nuclease ...
14
Developmental Modulation of Nonhomologous End Joining in Caenorhabditis elegans
... T WO main pathways exist for the repair of radiation- induced DNA double-strand breaks (DSBs): ho- mologous recombination (HR) and nonhomologous end joining (NHEJ; L ieber et al. 2003). HR utilizes ...
17
Reciprocal Translocations in Saccharomyces cerevisiae Formed by Nonhomologous End Joining
... B ALANCED translocations are common findings in generated between dispersed repeated sequences or be- leukemias, lymphomas, and sarcomas and in many tween heteroalleles by homologous recombination cases are thought to be ...
12