Nuclear protein
Isolation of nuclear proteins from flax (Linum usitatissimum L ) seed coats for gene expression regulation studies
... binding nuclear proteins suitable for gene expression study by gel shift experiments using seed coats of immature flax- seeds as starting ...a protein to bind a labeled DNA fragment in vitro, followed by ...
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mRNA export in the apicomplexan parasite Toxoplasma gondii: emerging divergent components of a crucial pathway
... the protein transport that relies on the Ran-GTPase sys- tem are conserved in the Apicomplexa (Additional file 1: Table S1), RanGAP, an essential factor of the Ran-GTP gra- dient, does not appear to be encoded by ...
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The Fission Yeast Schizosaccharomyces pombe Has Two Importin-α Proteins, Imp1p and Cut15p, Which Have Common and Unique Functions in Nucleocytoplasmic Transport and Cell Cycle Progression
... sensitive nuclear protein import assay, efficient nuclear import of an SV40 NLS fusion protein depended upon both Imp1p and Cut15p: One draw- back of using SV40 NLS-GFP-b-Gal to monitor ...
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Nuclear targeting of the tegument protein pp65 (UL83) of human cytomegalovirus: an unusual bipartite nuclear localization signal functions with other portions of the protein to mediate its efficient nuclear transport.
... direct nuclear import, confirming that this sequence con- tained a bona fide ...fusion protein to the nucleus. The Xenopus nuclear protein N1 has a bipartite NLS with an ami- no-terminal basic ...
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The Drosophila ash1 Gene Product, Which Is Localized at Specific Sites on Polytene Chromosomes, Contains a SET Domain and a PHD Finger
... Immunohistochemical localization of the ASHl pro- tein: Since the ASHl putative protein is presumed to be a nuclear protein by virtue of its multiple nuclear local[r] ...
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Triplex formation inhibits HER 2/neu transcription in vitro
... for nuclear protein binding by incubation with a crude nuclear ...prevents protein binding to the target site in the HER-2/neu promoter in ...
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Structural and functional domains of the myb oncogene: requirements for nuclear transport, myeloid transformation, and colony formation.
... We have examined the nuclear transport of the protein products of our v-myb deletion and truncation mutants and identified two indepen dent in nuclear accumulation of p48vmyb Mutants and[r] ...
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Bioinformatics Analysis of the Interaction between Coat Protein and Nuclear Shuttle Protein in Babuvirus
... and protein interactions play important roles in many biological processes and are responsible for carrying out the function of biological reg- ulatory network in living ...coat protein (CP) interacted with ...
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The Open Reading Frame 57 Gene Product of Herpesvirus Saimiri Shuttles between the Nucleus and Cytoplasm and Is Involved in Viral RNA Nuclear Export
... encoded nuclear cytoplasmic shuttle pro- teins have the ability to bind ...Rev protein binds to the RRE contained within the introns of these mRNAs mediated by an arginine-rich region ...cellular ...
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Performance Characteristics of the 10hp Induction Machine
... do nuclear export factors interact with transport-competent hnRNPs and what are the nuclear export signals (on the RNA, the proteins, or both)? Which components are involved directly in RNA export and which ...
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Fission Yeast Mog1p Homologue, Which Interacts With the Small GTPase Ran, Is Required for Mitosis-to-Interphase Transition and poly(A)+ RNA Metabolism
... Perturbations in the spi1p GTPase cycle of Schizosaccharomyces Shibuya, T., S. Tsuneyoshi, A. K. Azad, S. Urushiyama, Y. Ohshima pombe through its GTPase-activating protein and guanine nucleo- et al., 1999 ...
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The Methyltransferase-Like Domain of Chikungunya Virus nsP2 Inhibits the Interferon Response by Promoting the Nuclear Export of STAT1
... Flow cytometry. HEK293 cells transfected with the empty vector or various nsP2 expression vectors were treated with 1,000 U/ml IFN-  (I-4151; Sigma) for 15 min, washed twice in cold Dulbecco’s phosphate-buffered saline ...
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Localization and Mutational Analysis of the Nuclear and Aggregation-Prone Ime4 Protein in Saccharomyces cerevisiae
... the protein never left the wells (Figure ...case, protein extracts are made using protease inhibitors and no reducing agent, and an agarose gel is used instead of an SDS ...the protein extracts were ...
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Minute Virus of Mice Small Nonstructural Protein NS2 Interacts and Colocalizes with the Smn Protein
... SMN protein to self associate correlates with spinal muscular atrophy disease severity (18, ...the protein, murine and human SMN proteins are greater than 90% identical and share even greater degrees of ...
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Characterization of the Interaction between the Interferon-Induced Protein P56 and the Int6 Protein Encoded by a Locus of Insertion of the Mouse Mammary Tumor Virus
... of nuclear Int6 with ...P56-binding protein by a yeast two-hybrid screen of a HeLa cell cDNA ...Int6 protein, we obtained a complete human Int6 cDNA from Desbois et ...the protein because a ...
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Two RING Finger Proteins, the Oncoprotein PML and the Arenavirus Z Protein, Colocalize with the Nuclear Fraction of the Ribosomal P Proteins
... studies. Protein-protein interactions were demon- strated by coimmunoprecipitation ...with protein A-Sepharose (Pharmacia), the samples were washed twice in coimmu- noprecipitation buffer and ...P ...
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Herpes Simplex Virus Infection Induces Phosphorylation and Delocalization of Emerin, a Key Inner Nuclear Membrane Protein
... with nuclear actin (21, 24) and with the chromatin-associated protein BAF, where in vitro phosphorylation on S175 appears to affect bind- ing interaction (19), and these interactions could therefore be the ...
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Arginine-rich regions succeeding the nuclear localization region of the herpes simplex virus type 1 regulatory protein ICP27 are required for efficient nuclear localization and late gene expression.
... DNA-binding protein, ICP8 ...efficient nuclear localization of a pyruvate kinase fusion protein and was confirmed by the partial cyto- plasmic localization of viral recombinants with a deletion of ...
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Host Factors in Enterovirus 71 Replication
... hnRNP A1 has been shown to be involved in the replication of many viruses, such as murine hepatitis virus, hepatitis C virus, dengue virus, vesicular stomatitis virus, Sindbis virus, human papillomavirus 16, and human ...
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Loetzerich, Mark (2007): Analysis of the nuclear egress complex of mouse cytomegalovirus. Dissertation, LMU München: Fakultät für Biologie
... wt nuclear distribution upon isolated expression. No difference in protein stability compared to wild type M53/p38 was ...a protein-protein interaction might not be sufficient to define a ...
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