nucleic acid amplification-free
A formalin free method for stabilizing cells for nucleic acid amplification, hybridization and next generation sequencing
... Formalin can interact with macromolecules in mul- tiple ways. For example, formalin fixation induces not only protein–protein, but also DNA–protein cross-link- ing [4, 16]. The protein networks resulting from protein– ...
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Evaluation of non instrumented nucleic acid amplification by loop mediated isothermal amplification (NINA LAMP) for the diagnosis of malaria in Northwest Ethiopia
... non-instrumented nucleic acid amplification loop-mediated isothermal amplification (NINA-LAMP) compared to standard thick and thin film microscopy and nested PCR as gold standard for the ...
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Nucleic Acid Sequence Based Amplification Assay for Human Papillomavirus mRNA Detection and Typing: Evidence for DNA Amplification
... of nucleic acid sequence-based amplification (NASBA)-based HPV detection using HPV DNA plasmids (HPV type 16 [HPV16], HPV18, HPV31, HPV33, and HPV45) and nucleic acid extracts of ...
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Nuclease Resistant Single Stranded DNA Controls for Nucleic Acid Amplification Assays
... stable with no further decrease in signal recovery. There are at least two explanations for this transient response. First, misas- sembled or fragmented nrssDNA could be less nuclease resis- tant, leading to initial ...
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Detection of Mycoplasma pneumoniae in Spiked Clinical Samples by Nucleic Acid Sequence Based Amplification
... 25°C. Nucleic acids were extracted, and NASBA was performed. Nucleic acid ...extraction. Nucleic acids were extracted as described by Boom et ...The nucleic acid-silica complex ...
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Nucleic Acid Amplification Assays for Detection of La Crosse Virus RNA
... negative amplification controls containing 5 l of RNase- and DNase-free water, instead of extracted RNA, were included with each group of samples ...instructions. Amplification and fluores- cence ...
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Individual Donor Nucleic Acid Amplification Testing for Detection of West Nile Virus
... Evaluation of specificity. Donor plasma samples from frozen archive plates, collected during the WNV-free season, were screened to assess the specificity, false-positive rate, and IC failure rate of our WNV-NAT ...
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Impact of the introduction of a nucleic acid amplification test for Clostridium difficile diagnosis on stool rejection policies
... of free toxins could best define true or severe CDI. The presence of free toxins was significantly associated with unfavorable CDI, with an increase in white blood cells and a higher mortality ...
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Design and Validation of Transcription Mediated Amplification Nucleic Acid Amplification Tests for Mycoplasma genitalium
... tissue culture flasks at 37°C. Two M. genitalium strains, SEA-1 and SEA-2, were isolated from men enrolled in a Seattle NGU study (27), adapted to cell free growth, single-colony cloned, and cultured in SP4 medium. ...
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Comparison of Three Nucleic Acid Amplification Tests for Detection of Chlamydia trachomatis in Urine Specimens
... different nucleic acid sequence than the initial ...by amplification inhibitors since, like AC2, it includes a target capture step in the sample preparation, and it did confirm four of the five ...
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Nucleic Acid Sequence Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples
... real-time nucleic acid sequence-based amplification (NASBA) and PCR, have been developed to detect Trypanosoma brucei parasites in blood for the diagnosis of human African trypanosomiasis ...of ...
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Detection of Dengue Viral RNA Using a Nucleic Acid Sequence Based Amplification Assay
... two amplification oligo- nucleotides, and 15% dimethyl sulfoxide ...The amplification oligonu- cleotides used to target the dengue virus RNA genome are given in Table ...These amplification ...
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Comparison of Nucleic Acid Amplification, Serology, and Microbiologic Culture for Diagnosis of Rhodococcus equiPneumonia in Foals
... nonendemic. Nucleic acid amplification technology has been useful for rapid and accurate diagnosis of other types of bacterial infections of human beings and domestic animals, including horses (3, ...
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Relative Accuracy of Nucleic Acid Amplification Tests and Culture in Detecting Chlamydia in Asymptomatic Men
... Resolution of discordant results. The same procedure was employed to eval- uate either PCR or LCR (henceforth called the “new test”). If the new test was positive on a urine specimen and culture was positive on a ...
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Utility of cartridge based nucleic acid amplification test in the diagnosis of extra pulmonary tuberculosis
... based nucleic acid amplification test and pleural effusion with pulmonary lesions (p ...for acid fast bacilli in 29 patients and negative in 155 ...
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Nucleic Acid Amplification Tests for Diagnosis of Neisseria gonorrhoeae and Chlamydia trachomatis Rectal Infections
... infections. Nucleic acid amplification tests (NAATs) have been found to be more sensitive than culture for detection of genital gonococcal and chlamydial infections, but there are limited comparative ...
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The source of SYBR green master mix determines outcome of nucleic acid amplification reactions
... PCR amplification can identify the presence of nonspecific amplicons [8, ...improved amplification may result from the increased extension temperature of the Roche PCR ...
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Evaluation of Nucleic Acid Amplification Tests as Reference Tests for Chlamydia trachomatis Infections in Asymptomatic Men
... Urine ligase chain reaction (LCR) and PCR tests and urethral swab culture were compared for their abilities to detect Chlamydia trachomatis infection in 3,639 asymptomatic men by using one-, two-, and three-test ...
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Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid
... cleic acid greatly helped us study biological evolution, gene analysis, disease diagnosis, as well as drug dis- ...clear acid. Among these analogs, peptide nucleic acid (PNA) and locked ...
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Nucleic Acid Amplification Test Quantitation as Predictor of Toxin Presence in Clostridium difficile Infection
... sensitive nucleic acid amplifica- tion test (NAAT) is followed by a specific toxin A and toxin B enzyme immunoassay (EIA) is among the most accurate methods for Clostridium difficile infection (CDI) di- ...
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